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Demecolcine-assisted Enucleation에 의한 소 형질 전환 핵이식란 생산
Production of Bovine Transgenic Somatic Cell Nuclear Transfer Embryos by Demecolcine-assisted Enucleation

조종기    (충남대학교 수의과대학   ); 손정민    (충남대학교 수의과대학   ); 이두수    (충남대학교 수의과대학   ); 윤기영    (   ); 신상태    (충남대학교 수의과대학  );
  • 초록

    본 연구는 소 형질 전환 체세포 핵이식에서 용이한 탈핵을 위해 demecolcine을 이용할 시 탈핵율과 핵이식란의 발육능을 높이기 위한 최적의 조건을 알아보고자 실시되었다. 도축장 유래 미성숙 난자를 18시간 체외성숙 후 제1극체가 확인된 성숙 난자를 0.1, 0.2, 0.4 및 0.8 ug/ml의 demecolcine이 첨가된 배지에서 1시간 더 처리한 다음 세포막이 돌출되어 있는 난자를 체세포 핵이식에 공여하여 각 군간 배반포로의 발육능을 비교하였다. Demecolcine 처리 후 핵이 포함된 셰포막의 protrusion rates를 각 군간 비교한 결과 0.2, 0.4 및 0.8 ug/ml 군에서 0.1 ug/ml 군보다 유의적으로 높았으며 (82.8, 86.2, 90.4 vs. 70.1%), conventional blind 방법과 비교한 결과 demecolcine를 이용한 군에서 유의적으로 높은 탈핵율을 보였다(75.3 vs. 96.2%; p


  • 주제어

    bovine .   demecolcine .   enucleation .   somatic cell nuclear transfer .   transgenic.  

  • 참고문헌 (36)

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