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카드뮴이 뇌혈관 내피세포에서의 $PGE^2$ 및 COX-2 발현에 미치는 영향
Cadmium-induced COX-2 Expression in Cerebrovascular Endothelial Cells

박동현   (아주대학교 의과대학UU0000892  ); 김영채   (아주대학교 의과대학UU0000892  ); 문창규   (서울대학교 약학대학UU0000691  ); 정이숙   (아주대학교 의과대학UU0000892  ); 백은주   (아주대학교 의과대학UU0000892  ); 문창현   (아주대학교 의과대학UU0000892  ); 이수환   (아주대학교 의과대학UU0000892  );
  • 초록

    In order to get insight into the mechanism of cadmium (Cd)-induced brain injury, we investigated the effects of Cd on the induction of COX-2 in bEnd.3 mouse brain endothelial cells. Cd induced COX-2 expression and $PGE_2$ release, which were attenuated by thiol-reducing antioxidant N-acetylcysteine (NAC) indicating oxidative components might contribute to these events. Indeed, Cd increased cellular reactive oxygen species (ROS) level and DNA binding activity of nuclear factor-kB (NF-kB), an oxidative stress sensitive transcription factor. Cd-induced $PGE_2$ production and COX-2 expression were significantly attenuated by Bay 11 7082, a specific inhibitor of NF-kB and by SB203580, a specific inhibitor of p38 mitogen activated protein kinase (MAPK). These data suggest that Cd induces COX-2 expression through activation of NF-kB and p38 MAPK, the oxidative stress-sensitive signaling molecules, in brain endothelial cells.

  • 주제어

    cadmium .   brain endothelial cell .   cyclooxygenase-2 .   oxidative stress.  

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  • 이 논문을 인용한 문헌 (2)

    1. Seok, Sun-Mi ; Lee, Tae-Gu ; Kim, Young-Chae ; Moon, Chang-Hyun ; Baik, Eun-Joo ; Jung, Yi-Sook ; Lee, Soo-Hwan 2007. "Cadmium-induced E-cadherin Expression in Cerebrovascular Endothelial Cells" 환경독성학회지 = Journal of environmental toxicology, 22(2): 137~145     
    2. Jo, Hyeon-Jin ; Park, Sun-Dong 2014. "Anti-oxidative and anti-inflammatory experiments of Talmyung-san in RAW264.7 cells" 大韓韓醫學方劑學會誌 = Herbal formula science, 22(1): 79~92     

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