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Journal of plant biology = 식물학회지 v.50 no.6, 2007년, pp.681 - 686  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Rapid Shoot Propagation from Micro-Cross Sections of Kiwifruit (Actinidia deliciosa cv. 'Hayward')

Kim, Mi-Sun    (National Institute of Subtropical Agriculture, Rural Development Administration   ); Kim, Seong-Cheol    (National Institute of Subtropical Agriculture, Rural Development Administration   ); Moon, Doo-Young    (National Institute of Subtropical Agriculture, Rural Development Administration   ); Song, Kwan-Jeong    (Faculty of Bioscience and Industry, Cheju National University  );
  • 초록

    Kiwifruit shoots can be rapidly propagated through a micro-cross section (MCS) system we established here. Optimal culture conditions were determined for different explant types, section sizes, and concentrations of inorganic salts and plant growth regulators. Rates of survival and callus formation were higher in half-strength MS salts than in full-strength MS media. Similar performance (i.e., survival and callus formation) was achieved with section sizes of either $800{\mu}m$ or $1200{\mu}m$ . Proliferation efficiency was greatest when explants from stem tissue were cultured on 1/2 MS supplemented with $4.5{\times}10^{-3}{\mu}M$ 2,4-dichlo-rophenoxyacetic acid and $4.6{\times}10^{-1}{\mu}M$ zeatin. The number of shoots averaged 2.61 per explant, representing an efficiency of 94%. RAPD analysis revealed that the regenerated plants from our MCS system were genetically stable. These results show that the culturing of micro-cross sections from stem tissue is a powerful method for kiwifruit propagation.


  • 주제어

    kiwifruit .   normal shoots .   section sizes .   tissue types .   vibratory microtome.  

  • 참고문헌 (37)

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