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Korean journal of microbiology = 미생물학회지 v.43 no.4, 2007년, pp.304 - 310  
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Bacillus cereus 1-1 균주의 5-Aminolevulinic Acid (ALA) 생산
Production of 5-Aminolevulinic Acid (ALA) by Bacillus cereus 1-1

안경준   (서원대학교 과학교육과UU0000735  );
  • 초록

    Bacillus cereus 1-1 균주는 광이 없는 호기적 환경에서 levulinic acid와 같은 저해제 처리 없이도 5-aminolevulinic acid (ALA)를2 mM까지 생산하였다. B. cereus 1-1 균주는 TCY 배지에서 전 배양과 본 배양을 18시간 동안 지속하고, 배지의 pH가 6.8에 도달하는 대수기 후기에 acetic acid를 비롯한 유기산들을 16 mM 첨가하였을 때 많은 ALA를 생산하였으며, 본 배양 시작시 0.3% glucose를 첨가하는 것이 효과적이었다. Acetic acid 대신 glutamic acid를 첨가하였을 때 ALA 생산이 8시간 이상 지속되었고, $40\;{\mu}M$ 의 gabaculine을 첨가하면 생산이 현저히 저해되는 것으로 보아 B. cereus 1-1 균의 ALA 생산은 C-5 경로에 의함을 알 수 있었다.


    Bacillus cereus 1-1 strain produced 2 mM of ALA in the aerobic dark condition without any inhibitor like levulinic acid. The optimum culture conditions for the ALA production were that preculture and main culture were continued for 18 hr in TCY medium, and 16 mM of organic acids like acetic acid were added at the late log phase when the pH was 6.8. And the addition of 0.3% glucose was effective at the beginning of the main culture. ALA production was continued for more than 8 hr by the addition of glutamic acid instead of acetic acid, and was inhibited by addition of $40\;{\mu}M$ gabaculine seriously. These results confirmed that B. cereus 1-1 strain produced ALA through C-5 pathway.


  • 주제어

    5-aminolevulinic acid (ALA) .   Bacillus cereus .   C-5 pathway.  

  • 참고문헌 (47)

    1. Li, J.M., C.S. Russel, and D. Cosloy. 1989. Cloning and structure of the hemA gene of Escherichia coli K-12. Gene 82, 209-217 
    2. Murakami, K., Y. Hashimoto, and Y. Murooka. 1993. Cloning and characterization of the gene encoding glutamate 1-semialdehyde 2,1-aminomutase, which is involved in $\delta$-aminolevulinic acid synthesis in Propionibacterium freudenreichii. Appl. Environ. Microbiol. 59, 347-350 
    3. Petricek, M., L. Rutberg, I. Schroder, and L. Hederstedt. 1990. Cloning and characterization of the hemA region of the Bacillus subtilis chromosome. J. Bacteriol. 172, 2250-2258 
    4. Sasaki, K., T. Tanaka, N. Nishio, and S. Nagai. 1993. Effect of culture pH on the extracellular production of 5-aminolevulinic acid by Rhodobacter sphaeroides from volatile fatty acids. Biotechnol. Lett. 15, 859-864 
    5. Sasaki, K., M. Watanabe, T. Tanaka, and T. Tanaka. 2002. Biosynthesis, biotechnological production and applications of 5-aminolevulinic acid. Appl. Microbiol. Biotechnol. 58, 23-29 
    6. Schneegurt, M.A. and S.I. Beale. 1988. Characterization of the RNA required for biosynthesis of $\delta$-aminolevulinic acid from glutamate. Purification by anticodon-based affinity chromatography and determination that the UCC glutamate anticodon is general requirement for function in ALA biosynthesis. Plant Physiol. 86, 497-504 
    7. Takeya, H., T. Tanaka, T. Hotta, and K. Sasaki. 1997. Production methods and applications of 5-aminolevulinic acid. Porphyrins 6, 127-135 
    8. Bradshaw, R.E., S.W.C. Dixon, D.C. Raitt, and T.M. Pillar. 1993. Isolation and nucleotide sequence of the 5-aminolevulinic acid synthase gene from Aspergillus nidulans. Curr. Genet. 23, 501-507 
    9. Andersen, T., T. Briseid, T. Nesbakken, J. Ormerod, R. Sirevag, and M. Thorud. 1983. Mechanisms of synthesis of 5-aminolevulinate in purple, green and blue-green bacteria. FEMS Microbiol. Lett. 19, 303-306 
    10. Hansson, M., L. Rutberg, I. Schroder, and L. Hederstedt. 1991. The Bacillus subtilis hemAXCDBL gene cluster, which encodes enzymes of the biosynthetic pathway from glutamate to uroporphyrinogen III. J. Bacteriol. 173, 2590-2599 
    11. Asahara, N., K. Murakami, S. Korbrisate, Y. Hashimoto, and Y. Murooka. 1994. Cloning and characterization of the hemA gene for synthesis of $\delta$-aminolevulinic acid in Xanthomonas campestris pv. phaseoli. Appl. Microbiol. Biotechnol. 40, 846-850 
    12. Kuramochi, H., M. Konnai, T. Tanaka, and Y. Hotta. 1997. Method for improving plant salt tolerance. US patent 5-661-111 
    13. Neidle, E.L. and S. Kaplan. 1993. Expression of Rhodobacter sphaeroides hemA and hemT genes, encoding two 5-aminolevulinic acid synthetase isozymes. J. Bacteriol. 175, 2292-2303 
    14. Weinstein, J.D. and S.I. Beale. 1985. Enzymatic conversion of glutamate to $\delta$-aminolevulinate in soluble extracts of the unicellular green algae, Chlorella vulgaris. Archiv. Biochem. Biophy. 237, 454-464 
    15. Beale, S.J. and P.A. Castelfranco. 1984. The biosynthesis of $\delta$-aminolevulinic acid in higher plants. II. Formation of $^{14}C$ $\delta$-aminolevulinic acid from labeled precursors in greening plant tissues. Plant Physiol. 53, 297-303 
    16. Choi, C., B.S. Hong, H.C. Sung, H.S. Lee, and J.H. Kim. 1999. Optimization of extracellular 5-aminolevulinic acid production from Escherichia coli transformed with ALA synthetase gene of Bradyrhizobium japonicum. Biotechnol. Lett. 21, 551-554 
    17. Grimm, B. 1990. Primary structure of a key enzyme in plant tetrapyrrole synthesis : glutamate 1-semialdehyde aminotransferase. Proc. Natl. Acad. Sci. USA 87, 4169-4173 
    18. Kennedy, J.C., R.H. Pottier, and D.C. Pross. 1990. Photodynamic therapy with endogenous protoporphyrin IX : basic principles and present clinical experience. J. Photochem. Photobiol. 6, 143-148 
    19. Murakami, K., S. Korbsrisate, N. Asahara, Y. Hashimoto, and Y. Murooka. 1993. Cloning and characterization of the glutamate 1-semialdehyde 2,1-aminomutase gene from Xanthomonas campestris pv. phaseoli. Appl. Microbiol. Biotechnol. 38, 502-506 
    20. Verkamp, E. and B.A. Chelm. 1989. Isolation, nucleotide sequence, and preliminary characterization of the Escherichia coli K-12 hemA gene. J. Bacteriol. 171, 4728-4735 
    21. Nishikawa, S. and Y. Murooka. 2001. 5-Aminolevulinic acid : Production by fermentation, and agricultural and biomedical applications. Biotech. Genet. Eng. Rev. 18, 149-170 
    22. Tai, T.N., M.D. Moore, and S. Kaplan. 1988. Cloning and characterization of the 5-aminolevulinic acid synthetase gene(s) from Rhodobacter sphaeroides. Gene 70, 139-151 
    23. Hotta, Y. and K. Watanabe. 1999. Plant growth-regulating activities of 5-aminolevulinic acid. Syokobutu-no-Kagaku-Tyou-seti (Chemical regulation of plants). 34, 85-96 
    24. Sasikala, C., C.V. Ramana, and R. Rao. 1994. 5-aminolevulinic acid : A potential herbicide/insecticide from microorganisms. Biotechnol. Prog. 10, 451-459 
    25. Drolet, M., L. Peloquin, Y. Eccjelard, L. Cousiineau, and A. Sasarman. 1989. Isolation and nucleotide sequence of the hemA gene of Escherichia coli K-12. Mol. Gen. Genet. 216, 347-352 
    26. Grimm, B., A. Bull, and V. Btreu. 1991. Structural genes of glutamate 1-semialdehyde aminotransferase for porphyrin synthesis in cyanobacterium and Escherichia coli. Mol. Gen. Genet. 225, 1-10 
    27. Sasaki, K., S. Ikeda, Y. Nishizawa, and M. Hayashi. 1987. Production of 5-aminolevulinic acid by photosynthetic bacteria. J. Ferment. Technol. 65, 511-515 
    28. Rebeiz, C.A., A. Montazer-Zouhoor, H.J. Hopen, and S.M. Wu. 1984. Photodynamic herbicide. I. Concept and phenomology. Enzyme Microbial Technol. 6, 390-401 
    29. Sasaki, K., T. Tanaka, and S. Nagai. 1998. Use of photosynthetic bacteria for the production of SCP and chemicals from organic waste. In A.M. Martin (ed.), Bioconversion of waste materials to industrial products, second edition. Blackie Academic and Professional. pp. 247-291 
    30. Sato, K., K. Ishida, M. Shirai, and S. Shimizu. 1985. Occurrence and some properties of two types $\delta$-aminolevulinic acid synthase in a facultative methylotroph, Protaminobacter ruber. Agricul. Biol. Chem. 49, 3423-3428 
    31. Weinstein, J.D. and S.I. Beale. 1985. RNA is required for enzymatic conversion of glutamate to $\delta$-aminolevulinate by extracts of Chlorella vulgaris. Archiv. Biochem. Biophy. 239, 87-93 
    32. May, B.K., I.A. Brothwick, G. Srivastava, A. Pirola, and W.H. Elliott. 1986. Control of 5-aminolevulinic acid synthase in animals. Curr. Top. Cell Regul. 28, 233-261 
    33. Kaneko, S., T. Aoki, H. Nanato, N. Miyoshi, S. Houki, and Y. Fukuda. 1998. Intraoperative photodynamic diagnosis of human glioma using 5-ALA induced protoporphyrin IX. Iwamizawa-siritu Sougou Byouin-shi. 24, 71-79 
    34. Urban-Grimal, D., V. Ribes, and R. Labbe-Bois. 1984. Cloning by genetic complementation and restriction mapping of a yeast HEM1 gene coding for 5-aminolevulinate synthase. Curr. Genet. 8, 327-331 
    35. Sasaki, K., S. Ikeda, T. Konishi, Y. Nishizawa, and M. Hayashi. 1989. Influence of iron on the excretion of 5-aminolevulinic acid by photosynthetic bacterium, Rhodobacter sphaeroides. J. Ferment. Bioeng. 68, 378-381 
    36. Sasaki, K., T. Tanaka, Y. Nishizawa, and M. Hayashi. 1990. Production of a herbicide, 5-aminolevulinic acid, by Rhodobacter sphaeroides using the effluent waste from an anaerobic digestor. Appl. Microbiol. Biotechnol. 32, 727-731 
    37. Volland, C. and F. Felix. 1984. Isolation and properties of 5-aminolevulinic acid synthetase from the yeast Saccharomyces cerevisiae. Eur. J. Biochem. 142, 551-557 
    38. Li, J.M., H. Umanoff, R. Proenca, and S.D. Russel. 1988. Cloning of the Escherichia coli K-12 hemB gene. J. Bacteriol. 170, 1021-1025 
    39. Burnham, B.F. 1970. $\delta$-Aminolevulinic acid synthetase (Rhodopseudomonas sphaeroides). Methods Enzym. 17A, 195-204 
    40. Mariet, J., V.D. Werf, and J.G. Zeikus. 1996. 5-Aminolevulinic acid production by Escherichia coli containing the Rhodobacter sphaeroides hemA gene. Appl. Environ. Microbiol. 62, 3560-3566 
    41. Rebeiz, C.A., J.A. Juvik, and C.C. Rebeiz. 1988. Photodynamic insecticide. I. Concept and Phenomology. Pesticide Biochem. Physiol. 30, 11-27 
    42. Kim, H.S., G.G. Choi, M.N. Moon, Y.K. Yang, and Y.H. Rhee. 2002. Biosynthesis of polyhydroxyalkanoates and 5-aminolevulinic acid by Rhodopseudomonas sp. KCTC 1437. Kor. J. Microbiol. 38, 144-151     
    43. Sasaki, K., N. Noparatnaraporn, Y. Nishizawa, M. Hayashi, and S. Nagai. 1988. Production of herbicide, 5-aminolevulinic acid by a photosynthetic bacterium, Rhodobacter sphaeroides. Annual Reports of International Center of Cooperative Research in Biotechnology (Osaka University, Japan) 11, 375-378 
    44. Sasaki, K., T. Tanaka, Y. Nishizawa, and M. Hayashi. 1991. Enhanced production of 5-aminolevulinic acid by repeated addition of levulinic acid and supplement of precursors in photoheterotrophic culture of Rhodobacter sphaeroides. J. Ferment. Bioengineer. 71, 403-406 
    45. Houghton, J.D., L. Turner, and S.B. Brown. 1988. The effect of gabaculine on tetrapyrrole biosynthesis and heterotrophic growth in Cyanidium caldarium. Biochem. J. 254, 907-910 
    46. MaClung, R., J.E. Somervill, M.L. Guerinot, and B.K. Chelm. 1987. Structure of Bradyrhizobium japonicum gene hemA encoding 5-aminolevulinic acid synthase. Gene 54, 133-139 
    47. Stanley, J., D.N. Dowling, and W.J. Broughton. 1988. Cloning of hemA from Rhizobium sp. NGR234 and a symbiotic phenotype of a gene-directed mutant in diverse legume genera. Mol. Gen. Genet. 215, 32-37 

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