본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

한국육수학회지 = Korean journal of limnology v.40 no.4, 2007년, pp.489 - 494   피인용횟수: 3

Effects of Acute Metal Exposures on the Viability and mRNA Expression of Metallothionein in Hemibarbus mylodon Fry

Bang, In-Chul   (Department of Marine Biotechnology, Soonchunhyang UniversityUU0000848  ); Cho, Young-Sun   (Department of Aquaculture, Pukyong National UniversityUU0000598  ); Lee, Il-Rho   (Department of Marine Biotechnology, Soonchunhyang UniversityUU0000848  ); Nam, Yoon-Kwon   (Department of Aquaculture, Pukyong National UniversityUU0000598  );
  • 초록

    Transcriptional modulation of metallothionein (MT) during acute metal exposures (cadmium, copper or zinc) was examined in fry of Hemibarbus mylodon, a threatened fish species in Korean peninsula. Viability of H. mylodon fry was most affected by copper exposure (up to 79% of mortality at 1 ppm for 48 hours) and considerably by cadmium exposure (21 to 54% of mortality). On the other hand, Zn showed the least adverse effect on the viability (0 to 13% of mortality) of this species. Based on the semi-quantitative RT-PCR analysis, the stimulation of MT mRNA in response to metal exposures followed generally in a dose-dependent fashion where cadmium was the most potent inducer for the induction of MT transcripts in fry (up to more than 5-fold) while the lowest response was observed in zinc-exposed group (2-fold at maximum). From the exposure using environmentally realistic doses of cadmium (0 to 0.05 ppm for 24 hours), MT expression at mRNA level was also sensitively modulated toward upregulation up to more than 3-fold as relative to non-exposed control. Results from the present study would be a good basis for understanding the adaptive capacity and stress physiology of this endangered fish species during metal pollution.

  • 주제어

    Hemibarbus mylodon .   metallothionein expression .   heavy metal exposure .   viability.  

  • 참고문헌 (21)

    1. Alvarado, N.E., I. Quesada, K. Hylland, I. Marigomeza and M. Soto. 2006. Quantitative changes in metallothionein expression in target cell-types in the gills of turbot (Scophthalmus maxim us) exposed to Cd, Cu, Zn and after a depuration treatment. Aquat. Toxicol. 77: 64-77 
    2. Basha, P.S. and A.U. Rani. 2003. Cadmium-induced antioxidant defense mechanism in freshwater teleost Oreochromis mossambicus (Tilapia). Ecotoxicol. Environ. Saf. 56: 218-221 
    3. Cho, Y.S., B.N. Choi, E.M. Ha, K.H. Kim, S.K. Kim, D.S. Kim and Y.K. Nam. 2005. Shark (Scyliorhinus torazame) metallothionein: cDNA cloning, genomic sequence, and expression analysis. Mar. Biotechnol. (NY) 7: 350-362 
    4. Grosell, M., J. Blanchard, K.V. Brix. and R. Gerdes. 2007. Physiology is pivotal for interactions between salinity and acute copper toxicity to fish and invertebrates. Aquat. Toxicol. 84: 162-172 
    5. Haq, F., M. Mahoney and J. Koropatnick. 2003. Signaling events for metallothionein induction. Mutation Res. 533: 211-266 
    6. Jang, M.-H., M.C. Lucas and G.-J. Joo. 2003. The fish fauna of mountain streams in South Korean national parks and its significance to conservation of regional freshwater fish biodiversity. Biol. Conservation 114: 115-126 
    7. McGeer, J.C., S. Nadella, D.H. Alsop, L. Hollis, L.N. Taylor, D.G. McDonald and C.M. Wood. 2007. Influence of acclimation and cross-acclimation of metals on acute Cd toxicity and Cd uptake and distribution in rainbow trout (Oncorhynchus myhiss). Aquat. Toxicol. 84: 190-197 
    8. Olsson, P-E., P. Kling and C. Hogstrand. 1998. Mechanisms of heavy metal accumulation and toxicity in fish. In: Langston, W.J., M.J. Bebiano (Eds.), Metal Metabolism in Aquatic Environments. Chapman and Hall, London, p. 321-350 
    9. De Boeck, G., T.T.H. Ngo, K.V. Campenhout and R. Blust. 2003. Differential metallothionein induction patterns in three freshwater fish during sublethal copper exposure. Aquat. Toxicol. 65: 413-424 
    10. Risso-de Faverney, C., A. Devaux, M. Lafaurie, J.P. Girad, B. Bailly and R. Rahmani. 2001. Cadmium induces apoptosis and genotoxicity in rainbow trout hepatocytes through generation of reactive oxygen sepecies. Aquat. Toxicol. 53: 65-76 
    11. Kim, K.Y., S.Y. Lee, Y.S. Cho, I.C. Bang, D.S. Kim and Y.K. Nam. 2007. Characterization and phylogeny of two B-cytoskeletal actins from Hemibarbus mylodon (Cyprinidae, Cypriniformes), a threatened fish species in Korea. DNA Seq. (in press; DOI: 10.1080/10425170701445691) 
    12. Matsuo, A.Y.O., C.M. Wood and A.L. Val. 2005. Effects of copper and cadmium on ion transport and gill metal binding in the Amazonian teleost tambaqui (Colossoma macropomum) in extremely soft water. Aquat. Toxicol. 74: 351-364 
    13. Pierron, F., M. Baudrimont, A. Bossy, J.P. Bourdineaud, D. Brethes, P. Elie and J.C. Massbuau. 2007. Impairment of lipid storage by cadmium in the European eel (Anguilla anguilla). Aquat. Toxicol. 81: 304-311 
    14. Coyle, P., J.C. Philcox, L.C. Carey and A.M. Rofe. 2002. Metallothionein: the multipurpose protein. Cell. Mol. Life Sci. 59: 627-647 
    15. Dang, Z., M.H.G. Berntssen, A.K. Lundebye, G. Flik, S.E. Wendelaar Bonga and R.A.C. Lock. 2001. Metallothionein and cortisol receptors expression in gills of Atlantic salmon, Salmo salar, exposed to dietary cadmium. Aquat. Toxicol. 53:91-101 
    16. Hermesz, E., M. Abraham and J. Nemcsok. 2001. Tissue-specific expression of two metallothionein genes in common carp during cadmium exposure and temperature shock. Comp. Biochem. Physiol. 128C: 457-465 
    17. Alexandra, L. and A. Hontela, 2004. A comparative assessment of the adrenotoxic effects of cadmium in two teleost species, rainbow trout, Oncorhynchus mykiss, and yellow perch, Perea flavescens. Aquat. Toxicol. 67: 13-21 
    18. Cho, Y.S., B.N. Choi, K.H. Kim, S.K. Kim, D.S. Kim, I.C. Bang and Y.K. Nam. 2006. Differential expression of CU/Zn superoxide dismutase mRNA during exposures to heavy metals in rockbream (Oplegnathus fasciatus). Aquaculture 253: 667-679 
    19. Kock, G., R. Hoper and S. Wograth. 1995. Accumulation of trace metals Cd, Pb, Cu, Zn in arctic char Salvelinus alpinus from oligotrophic alpine lakes: relation to alkalinity. Can. J. Fish. Aquat. Sci. 52: 2367-2376 
    20. Meinelt, T. R.C. Playle, M. Pietrock, B.K. Burnison, A. Wienke and C.E.W. Steinberg. 2001. Interaction of cadmium toxicity in embryos and larvae of zebrafish (Danio rerio) with calcium and humic substances. Aquat. Toxicol. 54: 205-215 
    21. Olsvik, P.A., P. Gundersen, R.A. Andersen and K.E. Zachariassen. 2001. Metal accumulation and metallothionein in brown trout, Salmo trutta, from two Norwegian rivers differently contaminated with Cd, Cu and Zn. Comp. Biochem. Physiol. 128C: 189-201 
  • 이 논문을 인용한 문헌 (3)

    1. Lee, Yoon-A ; Yun, Young-Eun ; Nam, Yoon-Kwon ; Bang, In-Chul 2008. "Genetic Diversity of Endangered Fish Hemibarbus mylodon (Cyprinidae) Assessed by AFLP" 韓國養殖學會誌 = Journal of aquaculture, 21(3): 196~200     
    2. 2009. "" Fisheries and aquatic sciences, 12(3): 179~184     
    3. 2010. "" Fisheries and aquatic sciences, 13(2): 112~117     

 저자의 다른 논문

  • 방인철 (74)

    1. 1998 "수온 및 광주기 조절에 의한 돌돔, Oplegnathus fasciatus의 산란 유도" 韓國養殖學會誌 = Journal of aquaculture 11 (2): 141~149    
    2. 1998 "한국산 꺽지 (Coreoperca herzi)의 산란습성 및 초기생활사" 韓國養殖學會誌 = Journal of aquaculture 11 (1): 49~58    
    3. 1999 "면역조직화학법을 이용한 점농어 (Lateolabrax sp.) 뇌에서 두 종류 (sGnRH, cGnRH-II) 의 생식소자극호르몬 분비호르몬의 동정" 한국수산학회지 = Journal of the Korean Fisheries Society 32 (3): 266~270    
    4. 2000 "체세포분열 억제성 자성발생 2배체 메기, Silurus asotus 유도" 韓國養殖學會誌 = Journal of aquaculture 13 (4): 359~362    
    5. 2000 "은어, Plecoglossus altivelis의 초기 생식소 형성 및 성분화" 韓國養殖學會誌 = Journal of aquaculture 13 (3): 215~222    
    6. 2000 "다슬기(Semisulcospira libertina libertina)의 번식주기" 발생과 생식 = Development and reproduction 4 (2): 175~180    
    7. 2000 "환경조절에 의한 돌돔 Oplegnathus fasciatus 성 성숙 유도" Korean journal of Ichthyology = 한국어류학회지 12 (1): 46~53    
    8. 2001 "점농어, Lateolabrax maculatus의 성 성숙에 관한 연구" 한국수산학회지 = Journal of the Korean Fisheries Society 34 (5): 526~535    
    9. 2001 "한국산 꺽지과 어류 3종의 세포유전학적 연구" 한국수산학회지 = Journal of the Korean Fisheries Society 34 (1): 17~20    
    10. 2001 "대농갱이 (Leiocassis ussuriensis)의 초기 생식소 형성 및 성분화" Korean journal of Ichthyology = 한국어류학회지 13 (4): 248~253    
  • 조영선 (12)

  • 이일로 (5)

  • Nam, Yoon-Kwon (73)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역


  • NDSL :

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기