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한국육수학회지 = Korean journal of limnology v.40 no.4, 2007년, pp.489 - 494   피인용횟수: 3

Effects of Acute Metal Exposures on the Viability and mRNA Expression of Metallothionein in Hemibarbus mylodon Fry

Bang, In-Chul   (Department of Marine Biotechnology, Soonchunhyang UniversityUU0000848  ); Cho, Young-Sun   (Department of Aquaculture, Pukyong National UniversityUU0000598  ); Lee, Il-Rho   (Department of Marine Biotechnology, Soonchunhyang UniversityUU0000848  ); Nam, Yoon-Kwon   (Department of Aquaculture, Pukyong National UniversityUU0000598  );
  • 초록

    Transcriptional modulation of metallothionein (MT) during acute metal exposures (cadmium, copper or zinc) was examined in fry of Hemibarbus mylodon, a threatened fish species in Korean peninsula. Viability of H. mylodon fry was most affected by copper exposure (up to 79% of mortality at 1 ppm for 48 hours) and considerably by cadmium exposure (21 to 54% of mortality). On the other hand, Zn showed the least adverse effect on the viability (0 to 13% of mortality) of this species. Based on the semi-quantitative RT-PCR analysis, the stimulation of MT mRNA in response to metal exposures followed generally in a dose-dependent fashion where cadmium was the most potent inducer for the induction of MT transcripts in fry (up to more than 5-fold) while the lowest response was observed in zinc-exposed group (2-fold at maximum). From the exposure using environmentally realistic doses of cadmium (0 to 0.05 ppm for 24 hours), MT expression at mRNA level was also sensitively modulated toward upregulation up to more than 3-fold as relative to non-exposed control. Results from the present study would be a good basis for understanding the adaptive capacity and stress physiology of this endangered fish species during metal pollution.


  • 주제어

    Hemibarbus mylodon .   metallothionein expression .   heavy metal exposure .   viability.  

  • 참고문헌 (21)

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  • 이 논문을 인용한 문헌 (3)

    1. Lee, Yoon-A ; Yun, Young-Eun ; Nam, Yoon-Kwon ; Bang, In-Chul 2008. "Genetic Diversity of Endangered Fish Hemibarbus mylodon (Cyprinidae) Assessed by AFLP" 韓國養殖學會誌 = Journal of aquaculture, 21(3): 196~200     
    2. 2009. "" Fisheries and aquatic sciences, 12(3): 179~184     
    3. 2010. "" Fisheries and aquatic sciences, 13(2): 112~117     

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