본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

농약과학회지 = The Korean journal of pesticide science v.11 no.4, 2007년, pp.320 - 330   피인용횟수: 8

담배가루이 Biotype B와 Q의 약제감수성과 효소활성 비교
Comparison of insecticide susceptibility and enzyme activities of biotype B and Q of Bemisia tabaci

김은희   (충북대학교 농업생명환경대학 식물의학과UU0001309  ); 성재욱   (농업과학기술원 농약평가과CC0131248  ); 양정오   (충북대학교 농업생명환경대학 식물의학과UU0001309  ); 안희근   (충북대학교 농업생명환경대학 식물의학과UU0001309  ); 윤창만   (충북대학교 농업생명환경대학 식물의학과UU0001309  ); 서미자   (충북대학교 농업생명환경대학 식물의학과UU0001309  ); 김길하   (충북대학교 농업생명환경대학 식물의학과UU0001309  );
  • 초록

    충북 진천군 이월면 장미재배지와 경남 밀양시 고추밭에서 채집한 담배가루이에 대해서 16S DNA 염기서열을 분석하였다. 각각의 PCR 산물을 EcoT14 I (Sty I)과 Stu I을 처리한 결과 경남 밀양시 고추밭에서 채집한 담배가루이는 Sty I 에서 555pp와 311bp 두 개의 단편이 만들어져 biotype Q임을 확인하였고, 충북진천군 장미재배지에서 채집한 담배가루이는 Stu I에서 560bp와 3060p 두 개의 단편이 만들어서 biotype B임을 확인하였다. 두 biotype에 대한 12종 살충제로 발육단계별 약제감수성, 침투이행성 및 잔효성을 비교하였고, esterase, acetylcholinesterase (AChE), glutathione S-transferase 등의 효소활성에 미치는 저해정도를 검토하였다. 두 biotype간에 약제감수성 차이가 있었고, biotype B가 Q보다 더 감수성이었다. 엽면침투이행성과 근부침투이행성 및 잔효성에서도 biotype B가 Q보다 더 감수성이었다. 유기인계인 fenitrothion과 카바메이트계인 fenothiocarb의 저해제에 대해서 esterase, acetylcholinesterase (AChE), glutathione S-transferase등의 효소활성에 미치는 영향을 검토한 결과, biotype Q가 B보다 활성이 높게 나타났다. 이상의 결과를 종합하여 볼 때 담배가루이 biotype Q가 B보다 약제에 대해서 저항성임을 알 수 있었다.


    Mitochondrial 16S DNA sequences of Bemisia tabaci which were collected on rose greenhouse of Iwol and Jinchen in Chungbuk and red pepper field of Miryang, Gyeongnam, were analyzed. The mtCOI PCR product of B. tabaci collected on red pepper field of Miryang were digested with EcoT14I (Sty I) into two fragments 555bp and 311bp, while the PCR product of B. tabaci collected on rose greenhouse of Iwol were digested with Sty I into two fragments of 560bp and 306bp. As a result, B. tabaci collected on red pepper reveal biotype Q and those on rose greenhouse was biotype B. These was difference between two biotypes in insecticide susceptibility, and the biotype B was more susceptible than biotype Q. As a result of foliar systemic test, root-uptake systemic test and residual effect, the biotype B was more susceptible. In case of inhibition effect on enzyme activities of fenitrothion (organophosphorous) and fenothiocarb (carbamate), those of biotype Q was higher than those of biotype B. These results indicate that biotype Q was more resistant than biotype B against 12 insecticides.


  • 주제어

    담배가루이 .   생태형 B와 Q .   살충제 .   엽침투이행 .   잔류효과.  

  • 참고문헌 (36)

    1. Ahm, K. S., K. Y. Lee, M. H. Choi, J. W. Kim and G. H. Kim (2001) Effect of temperature and host plant on development and reproduction of the sweetpotato whitefly, Bemisia tabaci (Hopmoptera: Aleyrodidae). Korean J. Appl. Entomol. 43:203-209 
    2. Bellows, T. S., T. M. Perring, R. J. Gill and D. H. Headrick (1994) Description of a species of Bemisia. Ann. Entomol. Soc. Am. 87: 195-206 
    3. Devine, G. J., I. Ishaaya, A. R. Horowitz and I. Denholm (1999) The response of pyriproxyfen-resistant and susceptible Bemisia tabaci (Homoptera: Aleyrodidae) to pyriproxyfen and fenoxycarb alone and in combination with piperonyl butoxide. Pestic. Sci. 55:405-411 
    4. Frohlich, D. R, I. Torres-Jerez, I. D. Bedford, P. G. Markham, and J.K. Brown (1999) A phylogeographical analysis of the Bemisia tabaci species complex based on mitochondrial DNA markers. Mol. Entomol. 8:1683-1691 
    5. Horowitz, A. R. Z. Mendelson, M. Cahill and I. Ishaaya (1999) Managing resistance to the insect growth regulator, pyriproxyfen, in Bemisia tabaci. Pestic. Sci. 55:272-232 
    6. Liu, T. X. and P. A. Stansly (1997) Effects of pyriproxyfen on three species of Encarsia formosa (Hymenoptera: Aphelinidae), endoparasitoids of Bemisia argentifolii (Homoptera: Aleyrodidae). J. Econ. Entomol. 43:227-232 
    7. Matsui, M. (1995) Efficiency of Encarsia formosa in suppressing population density of Bemisia tabaci on tomatoes in plastic greenhouse. Jpn. J. Appl. Entomol. Zool. 39:25-31 
    8. Palumbo, J. C, A. .Horowitz, N. Prabhaker (2001) Insecticidal control and resistance management for Bemisia tabaci. Crop Protection 20:739-765 
    9. Perring, T. M. (2001) The Bemisia tabaci species complex. Crop Protection 20:725-737 
    10. Rubinstein, G., S. Morin and H. Cosnek (1999) Transmission of tomato yellow leaf curl geminivirus to imidacloprid treated tomato plants by the whitefly, Bemisia tabaci (Hemoptera : Aleyrodidae). J. Econ. Entomol. 92:658-662 
    11. Van Asperen, K. J. (1962) A study of housefly esterase by means of sensitive colorimetric method. J. Insect Pathol. 8:401-416 
    12. Lee, M. H., S. Y. Kang, S. G. Lee, J. Y. Choi, G. S. Lee, W. Y. Kim, S. W. Lee, S. G. Kim and K. B. Uhm (2005) Occurrence of the B- and Q-biotypes of Bemisia tabaci in Korea. Korean J. Appl. Entomol. 44(3):169-175     
    13. Van Lenteren, J. C. and J. Woets (1988) Biological and integrated pest control in greenhouses. Ann. Rev. Entomol. 33:239-269 
    14. Cottage, E. L. and R. V. Gunning (2006) Buprofezin inhibits acetylcholinesterase activity in B-biotype Bemisia tabaci. Journal of molecular neuroscience : MN, 30 (1-2):39-40 
    15. Horowitz, A. R. Z. Mendelson and I. Ishaaya (1997) Effect of abamectin mixed with mineral oil on the sweetpotato whitefly (Homoptera: Aleyrodidae). J. Econ. Entomol. 90:349-353 
    16. Brown, J. K., D. R. Frohlich and R. C. Rosell (1995) The sweetpotato/silverleaf whiteflies: biotypes of Bemisia tabaci germ, or a species complex? Ann. Rev. Entomol. 40:511-534 
    17. Ueda, S. (2006) Simple and Rapid Detection by mtCOI PCR-RFLP to distinguish the Q biotype of Bemisia tabaci. Kyushu Plant Prot. Res. 52:44-48 
    18. Kim, G. H, Y. S. Lee, I. H. Lee and K. S. Ahn (2000) Susceptibility of sweetpotato whitefly, Bemisia tabaci, (Homoptera: Aleyrodidae) to commercially registered insecticides in Korea. Korean J. Pesticide Sci. 4(1):51-58 
    19. Prabhaker, N., N. C. Toscano, T.M. Perring, G. Nuessly, K. Kido and R. R. Youngman (1992) Resistance monitoring of the sweetpotato whitefly (Homoptera: Alcyrodidae) in the Imperial Valley of California. J. Econ. Entomol. 85:1063-1068 
    20. Ishaaya. I., and A. R. Horowitz (1995) Pyriproxyfen, a novel insect growth regulator for controlling whiteflies: mechanism and resistance (Homoptera: Aleyrodidae). Pestic. Sci. 43:227-232 
    21. Habig, W. H, M. J. Pabst and W. B. Jakoby (1974) Glutathione S-transferase: The first enzymatic step in mecapturic acid formation. J. BioI. Chem. 249:7130-7139 
    22. Dennehy, T. J. and L. Williams (1997) Management of resistance in Arizona cotton. Pestic. Sci. 51:398-406 
    23. Lee, M. L., S. B. Ahn, and W. S. Cho (2000) Morphological characteristics of Bemisia tabaci (Germadius) (Homoptera: Aleyrodidae) and discrimination of their Biotypes in Korea by DNA markers. Korean J. Appl. Entomol. 39:5-12     
    24. EPPO (2004) Diagnostic protocols for regulated pests, Bemisia tabaci. EPPO Bulletin 34:281-288 
    25. Susan, A. C. J. K. Brown and L. H. Donald (2003) Biochemical characterization of biotype-specific esterases in the whitefly, Bemisia tabaci Genn (Homoptera: Aleyrodidae). Insect Biochem. Mol. Biol. 24(7):723-728 
    26. Larew, H. G, J. J. Knodel-Montz, R. F. Webb, and J. D. Warthen (1985) Liriomyza trifolii (Burgess) (Diptera: Agromyzidae) control on chrysanthermum by neem seed extract applied to soil. J. Econ Entomol. 78:80-84 
    27. Burban, C., L. D. C. Fishpool, C. Fauquet, D. Fargette and J. C. Thouvenel (1992) Host associated biotypes within West African population of the whitefly Bemisia tabaci (Germ.) (Hom., Aleyrodidae). J. Appl. Entomol. 113:416-423 
    28. Ellman, G. L., K. D. Coutey, V. Andres, J. and B. C. Featherstone (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 7:88-95 
    29. Ha, T. K, I. C. Hwang, J. K. Kim, Y. H. Song, G. H. Kim and Y. M. Yu (2003) Toxicities and control effect of three insecticides to greenhouse whitefly, Trialeurodes vaporariorum and sweetpotato whitefly Bemisia tabaci (Homoptera: Aleyrodidae). Korean J. Pesticide Sci. 3:29-36 
    30. Prabhaker, N., N. C. Toscano, and T. J. Henneberry (1999) Comparison of neem, urea, and amitraz as oviposition suppressants and larvicides against Bemisia argentifolii (Homoptera: Aleyrodidae). J. Econ. Entomol. 92(1):40-46 
    31. SAS Institute, 2003. SAS/STAT User's Guide: Statistics, version 9.1. SAS Institute, Cary, N.C., U.S.A 
    32. Rauch. N. and R. Nauen (2004) Characterization and molecular cloning of a glutathione S-tranferase from the whitefly Bemisia tabaci (Homoptera: Aleyrodidae). Insect Biochem. Mol. Biol. 34:321-329 
    33. Bedford, I. D., R. W. Briddon, J. K. Briddon, R. C. Rosell and P. G. Markham (1994) Geminivirus transmission and biological characterization of Bemisia tabaci (Gennadius) biotypes from different geographic regions. Ann Appl. BioI. 125:311-325 
    34. Guirao, P., F. Beitia and J. L. Cenis (1997) Biotype determination of Spanish populations of Bemisia tabaci (Homoptera: Aleyrodidae). Bull. Entomol. Res. 87:587-593 
    35. Ueda, S. and J. Brown (2006) First report of the Q biotype of Bemisia tabaci in Japan by mitochondrial cytochrome oxidase I sequence analysis. Phytoparasitica 34:405-411 
    36. Byrne, D. N., P. K. van Bretzel, and C. J. Hoffman (1986) Impact of trap design and placement when monitoring for the banded winged whittly and sweetpotato whitefly(H). Environ. Entomol. 15:300-304 
  • 이 논문을 인용한 문헌 (8)

    1. Kwon, Yun-Hee ; Yang, Jeong-Oh ; Oh, Jeong-Hoon ; Noh, Doo-Jin ; Yoon, Chang-Mann ; Kim, Gil-Hah 2008. "Changes of Feeding Behavior of Sweetpotato Whitefly, Bemisia tabaci Correlated with the Residual Effect of Emamectin benzoate and Pyridaben" 농약과학회지 = The Korean journal of pesticide science, 12(4): 397~402     
    2. Yang, Jeong-Oh ; Kim, Eun-Hee ; Yoon, Chang-Mann ; Ahn, Ki-Su ; Kim, Gil-Hah 2009. "Comparison of Feeding Behavior of B and Q biotypes of Bemisia tabaci (Hemiptera: Aleyrodidae) against Red Pepper and Tomato Varieties" 한국응용곤충학회지 = Korean journal of applied entomology, 48(2): 179~188     
    3. Yoon, Yeo-Jun ; Yu, Yong-Man ; Lee, Min-Ho ; Han, Eun-Jung ; Hong, Sung-Jun ; Ahn, Nan-Hee ; Kim, Yong-Ki ; Jee, Hyung-Jin ; Park, Jong-Ho 2010. "Characterization of Lecanicillium lecanii Btab0l Isolated with Bioactivities to Tabacco Whitefly (Bemisia tabaci)" 한국응용곤충학회지 = Korean journal of applied entomology, 49(4): 417~422     
    4. Jeong, Jin-Won ; Moon, Sang-Rae ; Cho, Sun-Ran ; Shin, Yun-Ho ; Kim, Gil-Hah 2010. "Repellent Effect of Wild Mint Oil Against Sweetpotato Whitefly, Bemisia tabaci" 농약과학회지 = The Korean journal of pesticide science, 14(4): 433~439     
    5. 2010. "" Journal of the Korean Society for Applied Biological Chemistry, 53(5): 605~617     
    6. Yoon, Chang-Mann ; Cho, Sun-Ran ; Moon, Sang-Rae ; Shin, Youn-Ho ; Kim, Gil-Hah 2011. "Insecticidal Activities of Polymers and Surfactants Against Sweet Potato Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)" 농약과학회지 = The Korean journal of pesticide science, 15(2): 177~187     
    7. 2014. "" Mycobiology, 42(4): 346~352     
    8. Lee, Mun-Haeng ; Lee, Hee-Kyoung ; Lee, Hwan-Gu ; Lee, Sun-Gye ; Kim, Jeom-Soon ; Kim, Sung-Eun ; Kim, Young-Shik ; Suh, Jeung-Keun ; Youn, Young-Nam 2014. "Effect of Cyantraniliprole against of Bemisia tabaci and Prevention of Tomato Yellow Leaf Curl Virus (TYLCV)" 농약과학회지 = The Korean journal of pesticide science, 18(1): 33~40     

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
  • 한국농약과학회 : 저널
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 이용한 콘텐츠
이 논문과 함께 출판된 논문 + 더보기