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하악골 신장술에서 압축자극을 통한 골재생시 TGF-${\beta}1$, Osteonectin 및 BMP-4의 발현
EXPRESSION OF TGF-${\beta}1$, OSTEONECTIN AND BMP-4 ON BONE REGENERATION IN MANDIBULAR DISTRACTION OSTEOGENESIS COMBINATION WITH A COMPRESSION STIMULATION

박성진   (부산대학교 치과대학 구강악안면외과학교실UU0000613  ); 김욱규   (부산대학교 치과대학 구강악안면외과학교실UU0000613  ); 정인교   (부산대학교 치과대학 구강악안면외과학교실UU0000613  ); 황대석   (부산대학교 치과대학 구강악안면외과학교실UU0000613  ); 김용덕   (부산대학교 치과대학 구강악안면외과학교실UU0000613  ); 신상훈   (부산대학교 치과대학 구강악안면외과학교실UU0000613  ); 김철훈   (동아의료원 구강악안면외과HH0064755  ); 변준호   (경상대학교 의과대학 구강악안면외과UU0000114  );
  • 초록

    Distraction osteogenesis(DO) is a technique of lengthning bone including soft tissue by gradual separation of surgically divided bone surfaces. Distraction osteogenesis combination with a compression stimulation(DO-CO) was a new technique by authors to enhance new bone quality and to shorten the consolidation period. The purpose of this study was to compare DO with DO combined with compression force in efficiency by evaluating the expression of TGF- ${\beta}1$ , osteonectin and BMP-4 on bone regenerate in rabbit mandible. Fourty two rabbits were used for this experiment. On the control group, the distraction was carried out at the rate of 1 mm per day to obtain the amount of 8 mm distraction for 8 days. On the experimental group, the distraction was carried out at the rate of 1 mm per day for 10 days, 3 days-latency period, and then the compression was carried out as counter direction 1 mm per day for 2 days. After 0 day, 5 days, 13 days, 20 days, 27 days, 34 days and 41 days, three rabbits on each group were sacrificed and the distracted portion of mandible were cut and treated for RT-PCR observation. The level of expression of TGF- ${\beta}1$ and osteonectin were shown more and longer expression in the experimental group than in the control group. The expression of BMP-4 was maintained with high level during the entire experimental period in both groups. These findings suggested that DO with compression stimulation could be a favorable technique for obtaining a good new bone quality.


  • 주제어

    Distraction osteogenesis .   Compression .   TGF- ${\beta}1$ .   Osteonectin .   BMP-4.  

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