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Journal of microbiology and biotechnology v.17 no.12, 2007년, pp.1996 - 2004   피인용횟수: 3
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Optimization of Culture Medium for Lactosucrose ($^4G-{\beta}$-D-Galactosylsucrose) Production by Sterigmatomyces elviae Mutant Using Statistical Analysis

Lee, Jong-Ho   (Department of Chemical and Biological Engineering, Korea UniversityUU0000159  ); Lim, Jung-Soo   (Digital Applicances R&D Team, Samsung Electronics Co. Ltd.CC0102000  ); Song, Yoon-Seok   (Department of Chemical and Biological Engineering, Korea UniversityUU0000159  ); Kang, Seong-Woo   (Department of Chemical and Biological Engineering, Korea UniversityUU0000159  ); Prak, Chul-Hwan   (Department of Chemical Engineering, Kwangwoon UniversityUU0000194  ); Kim, Seung-Wook   (Department of Chemical and Biological Engineering, Korea UniversityUU0000159  );
  • 초록

    In this study, the optimization of culture medium using a Sterigmatomyces elviae mutant was investigated using statistical analysis to increase the cell mass and lactosucrose ( $^4G-{\beta}$ -D-galactosylsucrose) production. In basal medium, the cell mass and lactosucrose production were 4.12 g/l and 140.91 g/l, respectively. However, because of the low cell mass and lactosucrose production, optimization of culture medium was carried out to increase the cell mass and lactosucrose production. Culture media were optimized by the S. elviae mutant using analysis of variance (ANOVA) and response surface methodology (RSM). Central composite designs using RSM were utilized in this investigation. Quadratic models were obtained for cell mass and lactosucrose production. In the case of cell mass, optimal components of the medium were as follows: sucrose 1.13%, yeast extract 0.99%, bactopeptone 2.96%, and ammonium sulfate 0.40%. The predicted maximum value of cell mass was about 5.20 g/l and its experimental value was 5.08 g/l. In the case of lactosucrose production, optimal components of the medium were as follows: sucrose 0.96%, yeast extract 1.2%, bactopeptone 3.0%, and ammonium sulfate 0.48%. Then, the predicted maximum value of lactosucrose production was about 194.12 g/l and the corresponding experimental value was about 183.78 g/l. Therefore, by culturing using predicted conditions, the real cell mass and lactosucrose production increased to 23.3% and 30.42%, respectively.


  • 주제어

    Medium optimization .   response surface methodology .   lactosucrose .   Sterigmatomyces elviae mutant.  

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  • 이 논문을 인용한 문헌 (3)

    1. 2008. "" Journal of microbiology and biotechnology, 18(11): 1797~1802     
    2. 2009. "" Journal of microbiology and biotechnology, 19(10): 1153~1160     
    3. Kim, Sung-Bong ; Lee, Ja-Hyun ; Oh, Kyeong-Keun ; Lee, Sang-Jun ; Lee, Jin-Young ; Kim, Jun-Seok ; Kim, Seung-Wook 2011. "Dilute Acid Pretreatment of Barley Straw and Its Saccharification and Fermentation" Biotechnology and bioprocess engineering, 16(4): 725~732     

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