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Journal of microbiology and biotechnology v.17 no.12, 2007년, pp.2081 - 2084   피인용횟수: 3
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Expression of ${\alpha}$-Galactosidase Gene from Leuconostoc mesenteroides SY1 in Leuconostoc citreum

Park, Jae-Yong   (Institute of Argriculture and Life Science, Gyeongsang National UniversityUU0000114  ); Jeong, Seon-Ju   (Institute of Argriculture and Life Science, Gyeongsang National UniversityUU0000114  ); Lee, Ae-Ran   (Division of Applied Life Science, Graduate School, Gyeongsang National UniversityUU0000114  ); Park, Ji-Yeong   (Division of Applied Life Science, Graduate School, Gyeongsang National UniversityUU0000114  ); Jeong, Woo-Ju   (Division of Applied Life Science, Graduate School, Gyeongsang National UniversityUU0000114  ); Kim, Jeong-Hwan   (Institute of Argriculture and Life Science, Gyeongsang National UniversityUU0000114  );
  • 초록

    A 2.5 kb aga gene encoding ${\alpha}$ -galactosidase ( ${\alpha}$ -Gal) from Leuconostoc mesenteroides SY1 was cloned into pSJE, an E. coli-Leuconostoc shuttle vector. The recombinant plasmid, pSJEaga, was introduced into Leuconostoc citreum KCTC3526 (ATCC49370) by electroporation. Transcription level of aga was the highest in cells grown on raffinose (1%, w/v) followed by cells grown on galactose, melibiose, fructose, glucose, and sucrose. Western blot using antibodies against ${\alpha}$ -Gal showed similar results to slot-blot results and enzyme activity measurements. All the results indicated that the aga was successfully expressed in L. citreum and its transcription was under the carbon catabolite repression (CCR).


  • 주제어

    ${\alpha}$-Galactosidase .   gene expression .   Leuconostoc citreum .   CCR.  

  • 참고문헌 (14)

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    13. Jeong, S. J., J. Y. Park, J. H. Kim, G. M. Kim, J. Chun, J. H. Lee, D. K. Chung, and J. H. Kim. 2006. Transformation of Leuconostoc mesenteroides SY1, a strain isolated from kimchi. J. Microbiol. Biotechnol. 16: 149-152     
    14. Park, J., M. Lee, J. Jung, and J. Kim. 2005. pIH01, a small cryptic plasmid from Leuconostoc citreum IH3. Plasmid 54: 184-189 
  • 이 논문을 인용한 문헌 (3)

    1. 2008. "" Journal of microbiology and biotechnology, 18(11): 1792~1796     
    2. 2008. "" Journal of food science and nutrition, 13(3): 190~195     
    3. 2008. "" Food science and biotechnology, 17(5): 1115~1118     

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