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유아 분변에서 분리한 Lactobacillus acidophilus의 특성 및 표면 단백질 유전자 클로닝과 대장균 내에서의 발현
Characterization of a Lactobacillus acidophilus Strain Isolated from Korean Infant Feces and Cloning of Surface Layer Protein Gene slp and Its Expression in Escherichia coli

박명수   (안양과학대학 호텔조리영양학부UU0000919  ); 지근억   (서울대학교 생활과학대학 식품영양학과UU0000691  ); 유관희   (상지대학교 이공과대학 생명과학과UU0000668  ); 이시경   (건국대학교 생명환경과학대학UU0000050  ); 정원석   (건국대학교 이과대학 생명과학과UU0000050  ); 김진형   (건국대학교 이과대학 생명과학과UU0000050  ); 조명환   (건국대학교 이과대학 생명과학과UU0000050  ); 김수영   (건국대학교 이과대학 생명과학과UU0000050  );
  • 초록

    A Lactobacillus sp. has been isolated from infant feces and characterized according to its physiological properties and identified as Lactobacillus acidophilus KLA1012. A gene coding surface layer protein (SLP) has been cloned and the sequence has been determined. The nucleotide sequence of slpA was 1,338 bp in size and was identical to that of L. acidophilus ATCC 4356 (100%). Amino acid sequence of SLP-A was deduced from the nucleotide sequence and it had signal sequence at N-terminal, consisting of positively charged amino acid mainly lysine. slpA was cloned and heterologously expressed in E. coli M15 and the 45.2 kDa surface-layer protein band was examined by SDS-PAGE and confirmed by Western blotting using polyclonal antibody against L. acidophilus KLA 1012 SLP-A protein.


  • 주제어

    Lactobacillus acidophilus .   surface layer protein gene .   nucleotide sequence.  

  • 참고문헌 (28)

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