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식물뿌리내부에 존재하는 지베렐린 생산균 분리와 동정
Isolation and Identification of Fungal Strains Producing Gibberellins from the Root of plants

임순옥   (경북대학교 미생물학과UU0000096  ); 이진형   (경북대학교 미생물학과UU0000096  ); 수메라 아프잘 칸   (경북대학교 미생물학과UU0000096  ); 이인중   (경북대학교 농학과UU0000096  ); 이인구   (경북대학교 농화학과UU0000096  ); 이경수   (영남이공대학 식음료조리계열UU0000957  ); 김종국   (경북대학교 미생물학과UU0000096  );
  • 초록

    249 fungal strains were isolated from the roots of 26 plants, and the production of GAs was spectrophotometric ally examined. As a result 76 fungal strains were shown to produce GAs. Bioassay of culture broth from seventy six fungal strains producing GAs was carried out with waito-c rice, that is dwarf rice. The seventy six fungi with GAs-producing activity were incubated for seven days in 40 mL of Czapek's liquid medium at $30^{\circ}C$ and 180 rpm, and the culture broth of fungi were treated on the 2-leaf rice sprout. Fifteen of these showed plant growth promoting activity and the amount of each GAs in the medium was measured by Gas chromatographymass spectrometer (GC-MS). Nine of these fungi were also identified by genetic analysis of the nucleotide sequences in the internal transcribed spacer region of the ribosomal DNA.


  • 주제어

    Gibberellin .   Gibberellins-producing fungi .   Bioassay .   Gas chromatography-mass spectrometer.  

  • 참고문헌 (14)

    1. Bottini, R., F. Cassan and P. Piccoli. 2004. Gibberellin production by bacteria and its involvement in plant growth promotion and yield increase. Appl. Microbiol. Biotechnol. 65: 497-503 
    2. Hedden, Peter and A. L. Phillips. 2000. Gibberellin metabolism: new insights revealed by the genes. Trands in Plant Sci. 5: 523-530 
    3. Kawanabe, Y., H. Yamane, T. Murayama, N. Takahashi, and T. Nakamura. 1983. Identification of gibberellin $A_3$ in mycelia Neurospora crassa. Agric. Biol. Chem. 47: 1693-1694 
    4. Tudzynski, B. 1999. Biosynthesis of gibberellins in Gibberella fujikuroi: biomolecular aspects. Appl. Microbiol. Biotechnol. 52: 298-310 
    5. Tudzynski B., M. Mihlan, M. Cecilia Rojas, P. Linnemannstons, P. Gaskin nd P. Hedden. 2003. Characterization of the final two genes of the gibberellin biosynthesis gene cluster of Gibberella fujikuroi: des and P450-3 encode GA4 desaturase and the 13-hydroxy1ase, respectively. J. Biol. Chem. 278: 28635-28643 
    6. Kawaide, H. and T. Sassa. 1993. Accumulation of gibberellin A, and the metabolism of gibberellin $A_9$ to gibberellin Al in a Phaeosphaeria sp. L 487 culture. Biosci. Biotech. Biochem. 57: 1403-1405 
    7. Rachev, R., V. Gancheva, S. Bojkova, C. Christov, and T. Zafirova, 1997. Gibberellin biosynthesis by Fusarium moniliforme in the presence of hydrophobic resin Amberlite XAD-2. Bulg. J. Plant Physi. 12: 24-31 
    8. Lee, Y. S., H. J. Son, I. H. Kim, and T. L. Mheen. 1983. Studies on the production of gibberellic acid. Kor. J. Appl. Microbiol. Bioeng. 3: 217-222 
    9. Rim, S. O., J. H. Lee, W. Y. Choi, S. K. Hwang, S. J. Suh, I. J. Lee, I. K. Rhee, and J. G. Kim. 2005. Fusarium prolifertum KGL0401 as a new gibberellin-producing fungus. J. Microbiol. Biotechnol. 15: 809-814 
    10. Lewis N. Mander. 2003. Twenty years of gibberellin research. Nat. Prod. Rep. 20: 49-69 
    11. Hedden, P., A. L. Phillips, M. C. Rojas, E. Carrera, and B. Tudzynski. 2002. Gibberellin biosynthesis in plants and fungi: A case of convergent evolution? J. Plant Growth Regul. 20: 319-331 
    12. Escamilla, E. M., L. Dendooven, I. P. Magana, R. Parra, and M. De la Torre. 2000. Optimization of gibberellic acid production by immobilized Gibberella fujikuroi mycelium in fluidized bioreactors. J. Biotechnol. 76: 147-55 
    13. Basiacik, K. S. and N. Aksoz. 2004. Optimization of carbonnitrogen ratio for production of gibberellic acid by Pseudomonas sp .. Pol. J. Microbiol. 53: 117-20 
    14. Choi, W. Y., S. O. Rim, J. H. Lee, J. M. Lee, I. J. Lee, K. J. Cho, I. K. Rhee, J. B. Kwon, and J. G. Kim. 2005. Isolation of gibberellins-producing fungi from the root of several Sesamum indicum plants. J. Microbiol. Biotechnol. 15: 22-28 
  • 이 논문을 인용한 문헌 (3)

    1. 2009. "" Journal of microbiology and biotechnology, 19(10): 1244~1249     
    2. You, Young-Hyun ; Hwang, Jeong-Sook ; Yoon, Hyeok-Jun ; Khan, Sumera Afzal ; Rim, Soon-Ok ; Bae, Jeong-Jin ; Choo, Yeon-Sik ; Lee, In-Jung ; Kong, Won-Sik ; Lee, Byung-Moo ; Kim, Jong-Guk 2010. "Plant Growth Promotion of Calystegia soldanella and Ischaemum anthephoroides by the Strain Penicillium citrinum KACC43900" 생명과학회지 = Journal of life science, 20(9): 1373~1377     
    3. You, Young-Hyun ; Yoon, Hyeok-Jun ; Woo, Ju-Ri ; Rim, Soon-Ok ; Lee, Jin-Hyung ; Kong, Won-Sik ; Kim, Jong-Guk 2011. "Diversity of Endophytic Fungi Isolated from the Rootlet of Pinus densiflora Colonized by Tricholoma matsutake" 한국균학회지 = The Korean journal of mycology, 39(3): 223~226     

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