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Asian-Australasian journal of animal sciences v.20 no.12, 2007년, pp.1820 - 1826   피인용횟수: 5
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Addition of Macromolecules to PZM-3 Culture Medium on the Development and Hatching of In vitro Porcine Embryos

Kim, J.Y.    (Infertility Medical Center of CHA General Hospital   ); Kim, S.B.    (Infertility Medical Center of CHA General Hospital   ); Park, M.C.    (Infertility Medical Center of CHA General Hospital   ); Park, H.    (Infertility Medical Center of CHA General Hospital   ); Park, Y.S.    (Kyongbuk Livestock Research Institute   ); Park, H.D.    (Department of Biotechnology, Daegu University   ); Lee, J.H.    (College of Medicine, Pochon CHA University   ); Kim, J.M.    (College of Medicine, Pochon CHA University  );
  • 초록

    In this study, we conducted various experiments in order to develop enhanced cultural conditions for in vitro-produced porcine embryos. All embryos were produced by in vitro maturation (IVM) and fertilization (IVF) of immature oocytes from abattoir-derived ovaries. In experiment 1, we cultured IVF embryos in 4 different groups, namely, 0% bovine serum albumin (BSA), 3% BSA, 0.05% Polyvinyl alcohol (PVA), and 0.5% Polyvinylpyrrolidone (PVP) added to the basal fluid cultural medium, Porcine zygote medium 3 (PZM-3). The rates of embryo development were higher in the group where the PZM-3 media had been supplemented with 3% BSA than the other groups. While not statistically significant, the percent of blastocysts and hatched blastocytes were 6.9% and 25.0% in the 3% BSA group vs. 1.2-6.4% and 0-16.7% in the other groups, respectively. In experiment 2, we added 10% fetal bovine serum (FBS) to PZM-3 on day 0 of culture and observed the development rate of blastocysts per day of culture from days 0 to 5. The development rate of blastocysts was higher at 15.6% on day 4 than on any other day, and was significantly higher than on day 0 or day 1 (p ${\rightarrow}$ day-4) PZM-3+10% FBS; 3) PZM-3+0.3% BSA ${\rightarrow}$ PZM-3+0.3% BSA+(day-4) FBS 10%; and 4) PZM-3+0.3% BSA+10% FBS (day0-day7). The development rates of blastocysts and hatched blastocysts were 21.5% and 53.1% in group 3, respectively, which was significantly higher than group 4 with respect to blastocyst development (5.2%, p $37.8{\pm}16.1$ than the other groups at $16.8{\pm}4.4$ - $30.1{\pm}10.9$ ; however, this was not significantly different. The results of this study showed that PZM-3 containing 0.3% BSA and supplemented with FBS during the later stage of culture on day 4 resulted in better TCNs and an increased rate of hatched blastocysts.


  • 주제어

    Porcine Embryos .   Macromolecule .   Blastocysts .   Cell Number.  

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  • 이 논문을 인용한 문헌 (5)

    1. 2008. "" Asian-Australasian journal of animal sciences, 21(3): 340~345     
    2. 2008. "" Asian-Australasian journal of animal sciences, 21(7): 980~987     
    3. 2009. "" Asian-Australasian journal of animal sciences, 22(8): 1117~1123     
    4. 2009. "" Asian-Australasian journal of animal sciences, 22(2): 174~180     
    5. 2009. "" Asian-Australasian journal of animal sciences, 22(4): 483~491     

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