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Molecular & cellular toxicology v.3 no.2, 2007년, pp.113 - 118  

Evaluation of the Genetic Toxicity of Synthetic Chemical (XVII) -In vitro Mouse Lymphoma Assay and In vitro Supravital Micronucleus Assay with 1, 2-Dichlorobenzene

Kim, Youn-Jung   (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and TechnologyCC0186926  ); Ryu, Jae-Chun   (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and TechnologyCC0186926  );
  • 초록

    Chlorobenzenes due to their acute toxicity and the capability of bioaccumulating are of great health and environmental concern. Especially, 1, 2-dichlorobenzene (CAS No. 95-50-1) is used for organic synthesis, dye manufacture, as a solvent and for other applications in chemical industry. Adverse effects of 1, 2-dichlorobenzene includes increases in liver and kidney weights and hepatotoxicity. In this study, we evaluated the genetic toxicity of 1, 2-dichlorobenzene with more advanced methods, in vitro mouse lymphoma assay $tk^{+/-}$ gene assay (MLA) and in vitro mouse supravital micronucleus (MN) assay. 1, 2-Dichlorobenzene appeared the significantly positive results and the induction of large mutant colonies only in the presence of metabolic activation system with MLA. But in vitro testing of 1, 2-dichlorobenzene yielded negative results with supravital MN assay. These results suggest that 1, 2-dichlorobenzene may play a mutagen rather than clastogen in vitro mammalian system.


  • 주제어

    1, 2-Dichlorobenzene .   In vitro Mouse Lymphoma Assay .   In vitro supravital micronucleus assay .   Genotoxicity.  

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