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Molecular & cellular toxicology v.3 no.2, 2007년, pp.113 - 118  

Evaluation of the Genetic Toxicity of Synthetic Chemical (XVII) -In vitro Mouse Lymphoma Assay and In vitro Supravital Micronucleus Assay with 1, 2-Dichlorobenzene

Kim, Youn-Jung   (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and TechnologyCC0186926  ); Ryu, Jae-Chun   (Cellular and Molecular Toxicology Laboratory, Korea Institute of Science and TechnologyCC0186926  );
  • 초록

    Chlorobenzenes due to their acute toxicity and the capability of bioaccumulating are of great health and environmental concern. Especially, 1, 2-dichlorobenzene (CAS No. 95-50-1) is used for organic synthesis, dye manufacture, as a solvent and for other applications in chemical industry. Adverse effects of 1, 2-dichlorobenzene includes increases in liver and kidney weights and hepatotoxicity. In this study, we evaluated the genetic toxicity of 1, 2-dichlorobenzene with more advanced methods, in vitro mouse lymphoma assay $tk^{+/-}$ gene assay (MLA) and in vitro mouse supravital micronucleus (MN) assay. 1, 2-Dichlorobenzene appeared the significantly positive results and the induction of large mutant colonies only in the presence of metabolic activation system with MLA. But in vitro testing of 1, 2-dichlorobenzene yielded negative results with supravital MN assay. These results suggest that 1, 2-dichlorobenzene may play a mutagen rather than clastogen in vitro mammalian system.

  • 주제어

    1, 2-Dichlorobenzene .   In vitro Mouse Lymphoma Assay .   In vitro supravital micronucleus assay .   Genotoxicity.  

  • 참고문헌 (30)

    1. US Department of Commerce. US exports, schedule E commodity by country (FT410/December 1980), Bureau of Census, Washington DC, pp. 2-74(1981) 
    2. Kumagai, S. & Matsunaga, I. Identification of urinary metabolites of human subjects exposed to o-dichlorobenzene. Int. Arch. Occup. Environ. Health 67:207- 209 (1995) 
    3. Kirkland, D. J. Genetic toxicology testing requirements: official and unofficial views from Europe. Environ. Mol. Mutagen. 2:8-14(1993). 
    4. Tennant, R. W. et al. Prediction of chemical carcinogenicity in rodents from in vitro genetic toxicity assays. Science 236:933-941 (1987) 
    5. Perocco, P., Bolognesi, S. & Alberghini, W. Toxic activity of seventeen industrial solvents and halogenated compounds on human lymphocytes cultured in vitro. Toxicol. Lett. 16:69-75 (1983) 
    6. Reustle, J. A. & Scribner, H. E. o-Dichlorobenzene; Myelotoxicity and cytogenetic study in rats. Report of the Toxicology Department, Rohm and Haas Company, Spring House, Pennsylvania. EPA/OTS Doc. No. 878212182, 1-71 (1979) 
    7. Amaral, O. C., Otero, R. & Grimalt, J. O. Volatile and semi-volatile organochlorine compounds in tap and riverine waters in the area of influence of a chlorinated organic solvent factory. Water Res. 30(8): 1876-1884(1996) 
    8. Applegate, M. L. et al. Molecular dissection of mutations at the heterozygous thymidine kinase locus in mouse lymphoma cells. Proc. Natl. Acad. Sci. USA 87:51-55 (1990) 
    9. Myhr, B. C. & Caspary, W. J. Chemical mutagenesis at the thymidine kinase locus in L5178Y mouse lymphoma cells: results for 31 coded compounds in the National Toxicology Program. Environ. Mol. Mutagen. 18:51-83 (1991) 
    10. Sofuni, T. Japanese guidelines for mutagenicity testing. Environ. Mol. Mutagen. 21:2-7 (1993) 
    11. Clements, J. Gene mutation assays in mammalian cells, In S. O'Hare and C. K. Atterwill (Ed.), Methods in Molecular Biology, Vol. 43: in vitro Toxicity Testing Protocols. Humana Press Inc. Totowa, NJ. 277- 286 (1990) 
    12. Matsui, S., Yamamoto, R. & Yamada, H. The Bacillus subtilis/microsome ret-assay for the detection of DNA damaging substances which may occur in chlorinated and ozonated waters. Wat. Sci. Tech. 21:875- 887 (1989) 
    13. Vogel, E. W. & Nivard, M. J. Performance of 181 chemicals in a Drosophila assay predominantly monitoring interchromosomal mitotic recombination. Mutagenesis 8:57-81 (1993) 
    14. Health Protection Branch Genotoxicity Committee. The assessment of mutagenicity: health protection branch mutagenicity guidelines. Environ. Mol. Mutagen. 21:15-37 (1993) 
    15. Zapata-Gayon, C., Zapata-Gayon, N. & Gonzalez- Angulo, A. Clastogenic chromosomal aberrations in 26 individuals accidentally exposed to ortho dichlorobenzene vapors in the National Medical Center in Mexico City. Arch. Environ. Health 37:231-235 (1982) 
    16. Marti, I., Lloret, R., Martin-Alonso, J. & Ventura, F. Determination of chlorinated toluenes in raw and treated water samples from the Llobregat river by closed loop stripping analysis and gas chromatography- mass spectrometry detection. J. Chromatogr. 1077(1):68-73 (2005) 
    17. Huybrechts, T., Dewulf, J. & Van Langenhove, H. Priority volatile organic compounds in surface waters of the southern North Sea. Environ. Pollut. 133(2): 255-264(2005). 
    18. Kitchin, K. T., Brown, J. L. & Kulkarni, A. P. Predictive assay for rodent carcinogenicity using in vitro biochemical parameters: operational characteristics and complementarity. Mutation Res. 266:253-272 (1992) 
    19. Robinson, W. D. et al. Statistical evaluation of bacterial/ mammalian fluctuation tests, in Statistical Evaluation of Mutagenicity Test Data (Kirkland, D. J., ed.). Cambridge University Press, Cambridge, UK, 102-140 (1990) 
    20. ECETOC. 'Risk assessment in marine environments'. Technical Report No. 82, Brussels, pp. 141, ISSN- 0773-8072-82 (2001) 
    21. Auletta, A. E., Dearfield, K. L. & Cimino, M. C. Mutagenicity test schemes and guidelines: US EPA office of pollution prevention and office of pesticide programs. Environ. Mol. Mutagen. 21:38-45 (1993) 
    22. BUA Report No. 53, VCH, September (1990) 
    23. OECD. SIDS Initial Assessment Report For SIAM 13 1, 2-DICHLOROBENZENE Bern, Switzerland, 6-9 November. UNEP PUBLICATIONS (2001). 
    24. Loveday, K. S., Anderson, B. E., Resnick, M. A. & Zeiger, E. Chromosome aberration and sister chromatid exchange tests in Chinese hamster ovary cells in vitro. V: Results with 46 chemicals. Environ. Mol. Mutagen. 16:272-303 (1990) 
    25. Ames, B. N., Durston, W. E., Yamasaki, E. & Lee, F. D. Carcinogens are mutagens: a simple test system combining liver homogenates for activation and bacteria for detection. Proc. Natl. Acad. Sci. USA 70: 2281-2285 (1973) 
    26. Heidrich, S. et al. Regionally contaminated aquiferstoxicological relevance and remediation options (Bitterfield case study). Toxicology 205(3):143-155 (2004) 
    27. Hayashi, M. et al. Isolation of micronuclei from mouse blood and fluorescence in situ hybridization with a mouse centromeric DNA probe. Mutation Res. 307:245-251 (1994) 
    28. Shelby, M. D., Erexson, G. L., Hook, G. J. & Tice, R. R. Evaluation of a three-exposure mouse bone marrow micronucleus protocol: results with 49 chemicals. Environ. Mol. Mutagen. 21:160-179 (1993) 
    29. Mohtashamipur, E., Triebel, R., Straeter, H. & Norpoth, K. The bone marrow clastogenicity of eight halogenated benzenes in male NMRI mice. Mutagenesis 2:111-113 (1987) 
    30. Hayashi, M. et al. The micronucleus assay with mouse peripheral blood reticulocytes using acridine orange-coated slides. Mutation Res. 245:245-249 (1990) 

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