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Journal of ginseng research = 高麗人參學會誌 v.31 no.4, 2007년, pp.203 - 209   피인용횟수: 4

효소처리에 의한 백삼 저분자 화합물의 V79-4 세포주에 대한 항산화 활성
Antioxidant activity of white ginseng extracts prepared by enzyme treatment on V79-4 cells induced by oxidative stress

김영찬   (한국식품연구원CC0186878  ); 임주혁   (한국식품연구원CC0186878  ); 노정해   (한국식품연구원CC0186878  ); 조장원   (한국식품연구원CC0186878  ); 이영경   (한국식품연구원CC0186878  );
  • 초록

    본 연구는 효소분해를 통하여 인삼의 저분자 화합물을 추출하고 추출수율, 총페놀 함량, V79-4 세포주를 이용한 항산화 활성을 시험하였다. 조추출물의 추출 수율은 29.5-76%였으며, 총페놀 함량은 0.45-2.21%로 처리 효소에 따라 차이를 보였으며, pectinase 처리구가 추출 수율과 총페놀 함량이 가장 높았다. V79-4 세포주에 대해서는 pectinase 처리구와 ${\alpha}$ -amylase 처리구에서 50% 이상의 세포 보호효과를 나타내었다. 세포내 항산화 관련 효소계의 활성을 시험한 결과 SOD활성은 처리 효소에 따른 유의적인 차이가 없었으나, CAT와 GPx 활성은 pectinase 처리구에서 유의적으로 높은 활성을 보였다. 또한 지질과산화 활성을 시험한 결과에서도 pectinase 처리구와 protease 처리구에서 50% 이상의 MDA 생성 억제를 보였다.


    This study examined the extraction yields, total phenolic compounds content and the antioxidant activities on V79-4 cells of white ginseng extracts prepared by enzyme treatment. Yields of crude extract were 29.5-76%, and total phenolic compounds content showed 0.45-2.2% according to enzyme treatments. Pectinase treatment group showed the highest values of extraction yields and total phenolic compounds content. Pectinase and a-amylase treatment groups protected V79-4 cell viability(above 50%) against $H_2O_2$ -induced oxidative damage. In the result of antioxidant enzyme activity evaluation in cells, enzyme treatments did not show the significant difference of SOD activity (p>0.05). However, pectinase treatment group exhibited increased CAT and GPx activities (p>0.05). Also, pectinase and protease treatment group inhibited MDA formation (>50%) in the lipid peroxidation protection experiment.


  • 주제어

    Korean ginseng .   enzyme treatment .   antioxidant activity .   V79-4.  

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  • 이 논문을 인용한 문헌 (4)

    1. Kim, Soo-Hyun ; Choi, Hyun-Jin ; Oh, Hyun-Taek ; Chung, Mi-Ja ; Cui, Cheng-Bi ; Ham, Seung-Shi 2008. "Cytoprotective Effect by Antioxidant Activity of Codonopsis lanceolata and Platycodon grandiflorum Ethyl Acetate Fraction in Human HepG2 Cells" 한국식품과학회지 = Korean journal of food science and technology, 40(6): 696~701     
    2. Im, Ga-Young ; Ma, Jin-Yeul ; Kim, Kun-Woo ; Choi, Jin-Kook ; Kang, Dong-Kyoon ; Kwon, Tae-Ryoung ; Jang, Se-Young ; Jeong, Yong-Jin 2011. "Quality Characteristics of 4 Year-old Ginseng by Enzymatic Hydrolysis Conditions" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 40(2): 229~234     
    3. Yoon, Bo-Ra ; Lee, Young-Jun ; Hong, Hee-Do ; Lee, Young-Chul ; Kim, Young-Chan ; Rhee, Young Kyoung ; Kim, Kyung-Tack ; Lee, Ok-Hwan 2012. "Inhibitory Effects of Panax ginseng C. A. Mayer Treated with High Temperature and High Pressure on Oxidative Stress" 한국식품영양학회지 = The Korean journal of food and nutrition, 25(4): 800~806     
    4. Gil, Sun-Kook ; Shin, Joong-Yup ; Kang, Dae-Il ; Ryu, Gi-Hyung 2016. "Effects of Extrusion and Enzyme Treatment on Extraction of β-Glucan from Agaricus blazei Murill" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 45(3): 380~385     

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