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The Production of Methyl Trans-cinnamate in the Submerged Cultures of Tricholoma matsutake Mycelia

Park, Youngki   (Div. Special Purpose Tree, Korea Forest Research InstituteCC0102761  ); Lee, Wi Young   (Div. Biotechnology, Korea Forest Reseach Institute  ); Ahn, Jin Kwon   (Div. Biotechnology, Korea Forest Reseach Institute  );
  • 초록

    Methyl trans-cinnamate is a significant flavor compound of Tricholoma matsutake. Attempts were made to produce this compound by culturing the mycelium using submerged culture. No methyl trans-cinnamate could be detected when the mycelium was cultured in the basal liquid medium. However, the addition of Pinus desiflora extracts to the medium, methyl trans-cinnamate was largely produced. To find out compounds or fractions inducing methyl trans-cinnamate, dichloromethane fraction obtained from the wood extracts of P. densiflora was subjected to column chromatography. Three sub-fractions were obtained from the $CH_2CI_2$ fraction. Submerged cultured mycelium treated with sub-fraction 1 has the highest content of methyl trans-cinnamate. Maximum methyl trans-cinnamate $(470.2{\mu}g/g)$ was obtained when the first sub-fraction of dichloromethane fraction of the P. densiflora wood extracts was added to the medium. This indicates that wood extracts of P. densiflora contains inducer of the methyl trans-cinnamate production in the T. matsutake submerged culture.


  • 주제어

    Tricholoma matstake mycelium .   methyl-trans cinnamate .   submerged culture .   Pinus densiflora extracts.  

  • 참고문헌 (13)

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