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Overexpression of the Acidic C-Terminus Region of HvRAF from Barley Confers Enhanced Cadmium Tolerance in Yeast

Jung, Jin-Wook    (School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University   ); Kim, Min-Kyun    (School of Agricultural Biotechnology and Center for Agricultural Biomaterials, Seoul National University  );
  • 초록

    The barley ERF-type transcription factor, HvRAF, has an acidic C-terminus domain that contains a hemopexin domain signature-like sequence. As an initial study to investigate the functioning of this domain, we cloned the partial HvRAF gene (pHvRAF) corresponding to the domain into a yeast/E. coli shuttle vector, pYES2. We then introduced the pYES2::pHvRAF recombinant plasmid into yeast (Saccharomyces cerevisiae strain INVSc1). Here, we report that yeast transformants harboring pYES2::pHvRAF show significant tolerance to cadmium stress.


  • 주제어

    cadmium stress .   ERF-type transcription factor .   Hordeum vulgare .   Saccharomyces cerevisiae.  

  • 참고문헌 (19)

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