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The plant pathology journal v.23 no.3, 2007년, pp.193 - 202   피인용횟수: 1

Characterization of a Novel Necrotic Response of Glycine max Line 'PI96188' to Xanthomonas axonopodis pv. glycines

Han, Sang-Wook    (Division of Plant Pathology, National Institute of Agricultural Science and Technology, Rural Development Administration   ); Choi, Min-Seon    (Division of Plant Pathology, National Institute of Agricultural Science and Technology, Rural Development Administration   ); Lee, Suk-Ha    (School of Plant Science, Seoul National University   ); Hwang, Duk-Ju    (Gene Expression Team, National Institute of Agricultural Biotechnology   ); Hwang, Byung-Kook    (Laboratory of Molecular Plant Pathology, Korea University   ); Heu, Sung-Gi    (Division of Plant Pathology, National Institute of Agricultural Science and Technology, Rural Development Administration  );
  • 초록

    Typical susceptible symptoms of the bacterial pustule disease caused by Xanthomonas axonopodis pv. glycines are pustule and chlorotic haloes that usually occur in leaves of Glycine max plants. The soybean genotype 'PI96188' showed an atypical response to all tested races X. axonopodis pv. glycines, accompanied with necrosis without chlorotic haloes on the underside of the necrotic symptoms. X. axonopodis pv. glycines 8ra grew to levels from 10 to 100 fold lower on PI96188 than on susceptible cultivar Jinjoo1, but 10-fold higher than on the resistant cultivar CNS. The chlorophyll content in PI96188 leaves remained unchanged until 12 days after bacterial infection. Ultrastructural observation showed that the infected leaf cells of PI96188 had intact normal chloroplasts compared to those of the susceptible cultivar Jinjoo1. Chloroplast degradation or the absence of chloroplasts in cells of the infected tissues was observed in Jinjoo1. Senescence-related ACS7 gene was significantly induced in PI96188 compared to those in Jinjoo1 at 2 days after inoculation. While photosynthesis-related rbcS gene showed the dramatic change in Jinjoo1, this gene was constitutively expressed in PI96188. However, expression of the defense-related genes, such as peroxidase and isoflavone synthase in the infected PI96188 leaves was similar to that in Jinjoo1. Together, these results suggest that the novel necrotic symptom in PI96188 is a kind of resistant response different from a typical hypersensitive response in the resistant genotypes.


  • 주제어

    bacterial pustule disease .   defense gene .   resistance .   soybean.  

  • 참고문헌 (33)

    1. Abeles, F. B., Morgan, P. W. and Saltveit, M. E. Jr. 1992. Ethylene in Plant Biology. San Diego, CA, USA. Academic Press 
    2. Greenberg, J. T. 1997. Programmed cell death in plant-pathogen interactions. Ann. Rev. Plant Physiol. Plant Mol. Biol. 48:525-545 
    3. Guiamet, J. J. and Giannibelli, M. C. 1994. Inhibition of the degradation of chloroplast membranes during senescence in nuclear 'stay green' mutants of soybean. Physiol. Plant 91:395-402 
    4. Hartwig, E. E. and Lehman, S. G 1951. Inheritance of resistance to bacterial pustule disease in soybeans. Agronomy J. 43:226-229 
    5. Jeong, D. J., Lim, W. S., Kwon, S. W. and Kim, K. H. 2005b. Identification of Glycine max genes expressed in response to soybean mosaic virus infection. Plant Pathol. J. 21:47-54     
    6. Kim, H. S., Park, H. J, Heu, S. and Jung, J. 2001a. Possible association of indole-3-acetic acid production by Xanthomonas axonopodis pv. glycines with development of pustule disease in soybean. Agric. Chemi. Biotech. 44: 173-176 
    7. Lee, B. K., Hong, J. K. and Hwang, B. K. 2001. Ultrastructure of compatible and incompatible interaction of pumpkin stems infected with Phytophthora capsici. Plant Pathol. J. 17:29-35 
    8. Linchtenthaler, H. K. 1987. Chlorophylls and carotenoids: Pigments or photosynthetic biomembranes. Methods Enrymol. 148:350-382 
    9. Patil, S. S. and Tam, L. Q. 1972. Mode of action of the toxin from Pseudomonas phaseolicola: I. toxin specificity, chlorosis, and ornithine accumulation. Plant Physiol. 49:803-807 
    10. Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, NY, U.S.A, Cold Spring Harbor Laboratory Press 
    11. Swing, J. G and Civerolo, E. L. 1993. Xanthomonas London, U. K. Champman & Hall 
    12. Turner, J. G 1981. Tabtoxin, produced by Pseudomonas tabaci, decreases Nicotiana tabacum glutamine synthetase in vivo and causes accumulation of ammonia. Physiol. Plant Pathol. 19:57-67 
    13. Fartushnyak, G B., Fartushnyak, A. T. and Mikhailov, V. G 1987. Bacterial disease of soybean in the northern forest steppe of the Ukraine and source of resistance to them. Vaskhnil 29:20-24 
    14. Braun, A. C. 1955. A study on the mode of action of the wildfire toxin. Phytopathology 45:659-64 
    15. Groth, D. E. and Braun, E. J. 1986. Growth kinetics and histopathology of Xanthomonas campestris pv. glycines in leaves of resistant and susceptible soybeans. Phytopathology 76:959-965 
    16. Hammond-Kosack, K. E. and Jones, J. D. G 1997. Plant disease resistance genes. Ann. Rev. Plant Physiol. Plant Mol. Biol. 48:575-607 
    17. Oh, C., Heu, S. and Choi, Y. 1999a. Sensitive and pathovar-specific detection of Xanthomonas campestris pv glycines by DNA hybridization and polymerase chain reaction analysis. Plant Pathol. J. 15:57-61 
    18. Sharma, A, Nair, P. M. and Pawer, S. E. 1993. Identification of soybean strains resistant to Xanthomonas campestris pv. glycines. Euphytica 67:95-99 
    19. Kim, J. H., Kim, W. T. and Kang, B. G 2001b. IAA and $N^6$-benzyladenine inhibit ethylene-regulated expression of ACC oxidase and ACC synthase genes in mungbean hypocotyls. Plant Cell Physiol. 42:1056-1061 
    20. Kim, C. S., Yi, S. Y., Lee, Y. K. and Hwang, B. K. 2000. Isolation and differential expression of an acidic PR-1 cDNA gene from soybean hypocotyls infected with Phytophthora sojae f. sp. glycines. Plant Pathol. J. 16:9-18 
    21. Oh, C. 1997. Isolation and characterization of pathogenicityrelated gene(s) from Xanthomonas campestris pv. glycines 8ra. Master Thesis, Seoul National University, Korea 
    22. Sinclair, J. B. and Backman, P. A 1989. Compendium of Soy bean Diseases. 3rd Ed. St. Paul., Minnesota, U.S.A APS Press 
    23. Stall, R. E. and Hall, C. B. 1984. Chlorosis and ethylene production in pepper leaves infected by Xanthomonas campestris pv. vesicatoria. Phytopathology 74:373-376 
    24. Jeong, D. J., Hwang, S. H., Kang, S. H., Choi, H. S., Park, J. W. and Kim, K. H. 2005a. Virus-induced gene silencing as tool for functional genomics in a Glycine max. Plant Pathol. J. 21: 158-163     
    25. Lee, H. S., Chae, Y. A, Park, E. H., Kim, Y. W., Yun, K. I. and Lee, SoH. 1997. Introduction, development, and characterization of supemodulating soybean mutant. I. Mutagenesis of soybean and selection of supemodulating mutant. Korean J. Crop. Sci. 42:247-253 
    26. Oh, C., Heu, S., Yoo, J. and Cho, Y. 1999b. An hrcU-homologous mutant of Xanthomonas campestris pv. glycines 8ra that lost pathogenicity on the host plant but was able to elicit the hypersensitive response on nonhosts. Mol. Plant-Microbe Interact 12:633-639 
    27. Manjaya, J. G and Pawar, S. E. 1999. New genes for resistance to Xanthomonas campestris pv. glycines in soybean [Glycine max (L.) Merr.] and their inheritance. Euphytica 106:205-208 
    28. Verma, V. D. 1990. Evaluation of soybean (Glycine max) germplasm for bacterial pustules caused by Xanthomonas campestris pv. glycines. Ind. J. Agric. Sci. 60:832-833 
    29. Yi, S. Y. and Hwang, B. K. 1998. Molecular cloning and characterization of a new basic peroxidase cDNA form soybean hypocotyls infected with Phytophthora sojae f.sp. glycines. Mol. Cells 8:556-564 
    30. Colye, P. D. 1989. Soybean Disease Atlas. 2nd Ed., Associated Printing Professionals, Inc., Louisiana Agricultural Experiment Station, Louisiana, USA 
    31. Gross, D. C. and Cody, Y. S. 1985. Mechanisms of plant pathogenesis by Pseudomonas species. Can. J. Microbiol. 31:403-410 
    32. Takamiya, K., Tsuchiya, T. and Ohta, H. 2000. Degradation pathway(s) of chlorophyll: what has gene cloning revealed? Trends Plant Sci. 5 :426-431 
    33. Durbin, R. D. 1971. Chlorosis-inducing pseudomonad toxins: Their mechanism of action and structure. In: Morphology and Biochemical Events in Plant Parasite Interaction eds. by S. Akai and S. Ouchi, pp. 369-385. Phytopathological Society of Japan, Tokyo 
  • 이 논문을 인용한 문헌 (1)

    1. 2011. "" Journal of crop science and biotechnology, 14(2): 119~123     

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