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Epigallocatechin Gallate가 인체 유방암 세포인 MDA-MB-231의 세포증식억제에 미치는 영향
Effect of Epigallocatechin Gallate on Inhibition of Cell Proliferation in MDA-MB-231 Human Breast Cancer Cells

홍은정    (단국대학교 식품영양학과   ); 김우경    (단국대학교 기초과학연구소  );
  • 초록

    본 연구는 녹차의 폴리페놀 성분 중에서 항암효과가 가장 크다고 알려진 EGCG가 인체 유방암 세포 MDA-MB-231의 세포증식억제 기전을 알아보고자 실시하였다. EGCG 처리 농도가 증가할수록 48시간 후에 유방암 세포의 증식이 유의적으로 감소되었다. 세포증식 관련 단백질인 $ErbB_2$ , $ErbB_3$ , Akt의 단백질 발현은 EGCG의 첨가농도 10 ${\mu}m$ 이상일 때부터 유의적으로 발현이 감소하였고, mRNA 발현은 5 ${\mu}m$ 이상부터 유의적으로 감소되었다. Py20, p-Akt 로 단백질의 인산화를 알아본 결과 EGCG 첨가농도가 증가할수록 $ErbB_2$ , $ErbB_3$ , Akt 의 인산화가 감소함을 확인할 수 있었다. 본 연구 결과를 종합해 보면 인체 유방암 세포 MDA-MB-231에서 EGCG는 암세포에서 과발현되는 $ErbB_2$ , $ErbB_3$ , Akt의 세포신호 전달과정 억제를 통하여 암세포의 증식을 억제시키는 것을 알 수 있었다.


    Epigallocatechin gallate (EGCG), a principal antioxidant derived from green tea, is one of the most extensively investigated chemopreventive phytochemicals. However, the effect of EGCG on proliferation in MDA-MB-231 breast cancer cell is not well known. We investigated the effect of EGCG on protein and mRNA expression related to cell proliferation in MDA-MB-231 human breast cancer cell lines. We cultured MDA-MB-231 cells in the presence of 0, 5, 10 and 20 ${\mu}m$ of EGCG. EGCG significantly inhibited the cancer cell proliferation (p $ErbB_2$ and $ErbB_3$ protein as well as mRNA expression. In addition, protein and mRNA expression of phosphorylated Akt and total Akt were significantly decreased (p $ErbB_2$ , $ErbB_3$ and Akt cell signaling.


  • 주제어

    epigallocatechin gallat .   breast cancer .   $ErbB_2$ .   $ErbB_3$ .   Akt.  

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  • 홍은정 (1)

    1. 2008 "Epigallocatechin Gallate가 인체 유방암 세포인 MDA-MB-231의 세포사멸에 미치는 영향" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition 37 (9): 1114~1119    
  • Kim, Woo-Kyoung (69)

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