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큰비쑥(Artemisia fukudo) 추출물의 murine macrophage RAW 264.7 세포에서 in vitro 항염효과
In vitro Anti-inflammatory Activity of the Artemisia fukudo Extracts in Murine Macrophage RAW 264.7 Cells

윤원종    ((재)제주하이테크산업진흥원 제주생물종다양성연구소   ); 이정아    ((재)제주하이테크산업진흥원 제주생물종다양성연구소   ); 김길남    ((재)제주하이테크산업진흥원 제주생물종다양성연구소   ); 김지영    ((재)제주하이테크산업진흥원 제주생물종다양성연구소   ); 박수영    ((재)제주하이테크산업진흥원 제주생물종다양성연구소  );
  • 초록

    본 연구는 쑥 추출물의 항염 활성이 prostaglandins 합성의 저해 및 pro-inflammatory cytokine의 억제기전과 관련이 있을 것으로 예상되어짐에 따라, 큰비쑥(A. fukudo)을 대상으로 80% EtOH로 추출하고 추출물을 극성에 따라 용매분획을 실시하여, 큰비쑥 에탄올 추출물 및 용매분획물들이 염증반응의 주체가 되는 대식 세포 계열인 RAW 264.7 세포에서 LPS 로 유도된 TNF- ${\alpha}$ , IL- $1{\beta}$ 그리고 IL-6와 같은 pro-inflammatory cytokine과 NO의 생성억제효과, 그리고 iNOS와 COX-2의 단백질 발현 억제효과 및 $PGE_{2}$ 생성 억제효과 등을 통해 알아보았다. 대식세포 계열인 RAW 264.7 세포에 LPS로 자극을 주고 큰비쑥 추출물을 처리하여 확인해본 결과, 추출물 및 분획물들이 다소 차이는 있었지만 TNF- ${\alpha}$ , IL- $1{\beta}$ 그리고 IL-6에서 생성억제 효과를 나타났다. 또한 헥산, 디클로로메탄 및 에틸아세테이트 분획물에서 NO의 생성억제 효과가 강하게 나타났으며, 헥산과 디클로로메탄 분획물에서는 iNOS, COX-2 및 $PGE_{2}$ 생성 억제 효과가 다른 분획물에 비해 강하게 나타났다. 이러한 결과는 큰비쑥에서 유효성분 추출을 통한 항염증 물질의 연구 또는 예방하거나 치료할 수 있는 염증 억제 성분의 분리 및 그 작용기전 연구에 중요한 기초 자료가 될 것이라 사료된다. 또한 큰비쑥 추출물로부터 염증억제 성분을 도출하고자 활성분획인 헥산과 디클로로메탄 분획물에 대하여 활성성분의 분리가 진행 중이다.

    The present study describes the preliminary evaluation of the anti-inflammatory activities of Artemisia fukudo extracts. The 80% ethanol extract of A. fukudo was sequentially fractionated with n-hexane, dichloromethane, ethylacetate, and butanol. In order to effectively screen for anti-inflammatory agents, we first examined the extracts' inhibitory effects on the production of pro-inflammatory cytokines activated with lipopolysaccharide. Moreover, we examined the inhibitory effects of the A. fukudo extracts on pro-inflammatory factors (NO, iNOS, COX-2, and $PGE_{2}$ ) in murine macrophage RAW 264.7 cells. The protein levels were determined by immunoblotting. Of the sequential solvent fractions, the n-hexane and dichloromethane fractions inhibited the mRNA expression of pro-inflammatory cytokines (TNF- ${\alpha}$ , IL- $1{\beta}$ , and IL-6), production of NO and $PGE_{2}$ , and the protein levels of iNOS and COX-2. These results suggest that A. fukudo may have signifIcant effects on inflammatory factors, and may be a potential anti-inflammatory therapeutic plant.

  • 주제어

    Artemisia fukudo .   pro-inflammatory cytokines .   NO .   $PGE_{2}$ .   iNOS .   COX-2.  

  • 참고문헌 (29)

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    1. Lee, Sang-Keug ; Lee, Hak-Ju ; Kang, Ha-Youg ; Choi, Don-Ha ; Jo, Hyun-Jin ; Lee, Tae-Seong 2009. "Studies on the Chemical Constituents Biological Activities of Mulberry Extracts" 목재공학 = Journal of the Korean wood science and technology, 37(1): 105~113     
    2. Jeoung, Young-Jun ; Choi, Se-Young ; An, Chi-Sun ; Jeon, Yun-Hee ; Park, Dong-Ki ; Lim, Beoung-Ou 2009. "Comparative Effect on Anti-Inflammatory Activity of the Phellinus linteus and Phellinus linteus Grown in Germinated Brown Rice Extracts in Murine Macrophage RAW 264.7 Cells" 韓國藥用作物學會誌 = Korean journal of medicinal crop science, 17(2): 97~101     
    3. Lee, Seung-Jun ; Cho, Han-Baek ; Kim, Song-Baeg ; Jang, Yun-Jeong ; Lee, Su-Jeong ; O, Kwang-Woo ; Choe, Chang-Min 2009. "Suppressive Effect of Yongdamsagantanggamibang on the Inflammatory Factors" 大韓韓方婦人科學會誌 = The Journal of Korean obstetrics & gynecology, 22(3): 51~65     
    4. Park, Yu-Hwa ; Lim, Sang-Hyun ; Ham, Hun-Ju ; Kim, Hee-Yeon ; Jeong, Haet-Nim ; Kim, Kyung-Hee ; Kim, Song-Mun 2010. "Isolation of Anti-inflammatory Active Substance β-Sitosterol from Seabuckthorn (Hippophae rhamnoides L.) Stem" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 39(7): 980~985     
    5. Lee, Kyoung-In ; Cho, Joo-Hyun ; Pyo, Byoung-Sik 2011. "Cytotoxicity and Antioxidative Activity of Artemisiae Argi Folium Alcoholic Extracts and Their Fractions" 韓國藥用作物學會誌 = Korean journal of medicinal crop science, 19(6): 421~425     
    6. Kim, Yon-Suk ; Lee, Seung-Jae ; Hwang, Jin-Woo ; Kim, Ee-Hwa ; Park, Pyo-Jam ; Jeong, Jae-Hyun 2012. "Anti-Inflammatory Effects of Extracts from Ligustrum ovalifolium H. Leaves on RAW264.7 Macrophages" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 41(9): 1205~1210     
    7. Choi, Hee Jung ; Choi, Young-Whan ; Baek, Sun-Yong ; Kim, Bong-Seon ; Ahn, Soon Cheol ; Rhee, Moon-Soo ; Yoon, Sik 2013. "Activity-guided Screening of Anti-inflammatory Compounds from the Hexane Extracts of Schisandra chinensis Fruit" 생명과학회지 = Journal of life science, 23(2): 311~318     
    8. Hwang, Cho-Rong ; Seo, Weon-Taek ; Jung, Min-Jung ; Shin, Jung-Hye 2013. "Antioxidant activity of the Sumaeyaksuk tea extracts prepared with different drying and extract conditions" 한국식품저장유통학회지 = Korean journal of food preservation, 20(4): 546~553     
    9. Ko, Myoung-Suk ; Bae, Kee-Hwa ; Lee, Mi-Hyun ; Kim, Nam-Young ; Lee, Yeon-Kyung ; Han, Myoung-Soo ; Song, Jae-Mo ; Song, Gwanpill 2014. "Effect of Seawater Concentration on Seed Germination and Seedling Growth of Artemisia fukudo" Journal of forest science, 30(1): 120~125     
    10. Kim, Eun-Ju ; Jang, Yeon-Jeong ; Kim, So-Young ; Choi, Hye-Sun ; Park, Shin-Young 2016. "Protective effects of quality certified traditional Doenjang in Korea on TNF-α-induced vascular inflammation in human umbilical vein endothelial cells" 한국식품저장유통학회지 = Korean journal of food preservation, 23(3): 378~386     
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