본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

In vitro 환경에서 엘라스틴을 혼합한 콜라겐 진피 지지체의 내구성
The Durability of Elastin-Incorporated Collagen Matrix for Dermal Substitute in Vitro Condition

유대현   (연세대학교 의과대학 성형외과학교실, 인체조직복원연구소UU0000936  ); 홍종원   (연세대학교 의과대학 성형외과학교실, 인체조직복원연구소UU0000936  ); 탁관철   (연세대학교 의과대학 성형외과학교실, 인체조직복원연구소UU0000936  );
  • 초록

    Purpose: Since the report of artificial dermis manufacturing method using collagen by Yannas in 1980, collagen has been effectively used as dermal substitute with its merits such as, lower antigeneicity, controllable biodegradation rate, and minimal inflammatory cytotoxic properties in the dermal tissue engineering field. However, weak mechanical durability was the main drawback of collagen dermal substitute. To improve its stability, mechanical or chemical cross-linking was used. Despite of such process, its clinical use was restricted due to weak durability. To improve the durability of collagen matrix, we designed elastin-incorporated collagen matrix and compared its durability with conventional collagen matrix. Methods: 15mm diameter with 4mm thick collagen dermal matrix was made according to Yannas protocol by mixing 0.5% bovine collagen and chondroitin-6-sulfate followed by degassing, freeze drying, dehydrodermal cross-linking and chemical cross-linking procedure. In elastin incorporated collagen matrix, same procedure was performed by mixing elastin to previous collagen matrix in 4:1 ratio(collagen 80% elastin 20%). In comparison of the two dermal matrix in vitro tests, matrix contracture rate, strain, tensile strength, was measured and stiffness was calculated from comparative analysis. Results: In terms of matrix contracture, the elastin-incorperated added collagen dermis matrix showed 1.2 times more contraction compared to conventional collagen matrix. However, tensile strength showed 1.6 times and stiffness showed 1.6 times increase in elastin-incorporated matrix. Conclusion: Elastin incorperated collagen matrix manufactured by our team showed increased durability due to improvement in tensile strength and stiffness compared to previous collagen matrix( $Integra^{(R)}$ ).


  • 주제어

    Elastin .   Collagen .   Dermis .   Durability.  

  • 참고문헌 (17)

    1. Petite H, Rault I, Huc A, Menasche P, Herbage D: Use of the acyl azide method for cross-linking collagen- rich tissues such as pericardium. J Biomed Mater Res 24: 179, 1990 
    2. Golomb G, Schoen FJ, Smith MS, Linden J, Dixon M, Levy RJ: The role of glutaraldehyde-induced cross- links in calcification of bovine pericardium used in cardiac valve bioprostheses. Am J Pathol 127: 122, 1987 
    3. Kang HW, Tabata Y, Ikada Y: Fabrication of porous gelatin scaffolds for tissue engineering. Biomaterials 20: 1339, 1999 
    4. Ma L, Gao C, Mao Z, Zhou J, Shen J, Hu X, Han C: Collagen/chitosan porous scaffolds with improved biostability for skin tissue engineering. Biomaterials 24: 4833, 2003 
    5. Rosenbloom J, Abrams WR, Mecham R: Extracellular matrix 4: the elastic fiber. FASEB J 7: 1208, 1993 
    6. Chu CS, McManus AT, Matylevich NP, Goodwin CW, Pruitt BA Jr: Integra as a dermal replacement in a meshed composite skin graft in a rat model: a one- step operative procedure. J Trauma 52: 122, 2002 
    7. Yannas IV, Burke JF, Gordon PL, Huang C, Rubenstein RH: Design of an artificial skin. II. Control of chemical composition. J Biomed Mater Res 14: 107, 1980 
    8. Lew DH, Liu PH, Orgill DP: Optimization of UV cross-linking density for durable and nontoxic collagen GAG dermal substitute. J Biomed Mater Res B Appl Biomater 82: 51, 2007 
    9. Suh H, Lee JE: Behavior of fibroblasts on a porous hyaluronic acid incorporated collagen matrix. Yonsei Med J 43: 193, 2002 
    10. Torres DS, Freyman TM, Yannas IV, Spector M: Tendon cell contraction of collagen-GAG matrices in vitro: effect of cross-linking. Biomaterials 21: 1607, 2000 
    11. Huang-Lee LL, Cheung DT, Nimni ME: Biochemical changes and cytotoxicity associated with the degradation of polymeric glutaraldehyde derived crosslinks. J Biomed Mater Res 24: 1185, 1990 
    12. Oliver RF, Grant RA, Cox RW, Cooke A: Effect of aldehyde cross-linking on human dermal collagen implants in the rat. Br J Exp Pathol 61: 544, 1980 
    13. Koide T, Daito M: Effects of various collagen crosslinking techniques on mechanical properties of collagen film. Dent Mater J 16: 1, 1997 
    14. Ng KW, Tham W, Lim TC, Werner Hutmacher D: Assimilating cell sheets and hybrid scaffolds for dermal tissue engineering. J Biomed Mater Res A 75: 425, 2005 
    15. Yannas IV, Burke JF: Design of an artificial skin. I. Basic design principles. J Biomed Mater Res 14: 65, 1980 
    16. Ruszczak Z: Effect of collagen matrices on dermal wound healing. Adv Drug Deliv Rev 55: 1595, 2003 
    17. Stenzel KH, Miyata T, Rubin AL: Collagen as a biomaterial. Annu Rev Biophys Bioeng 3: 231, 1974 

 저자의 다른 논문

  • 유대현 (46)

    1. 1996 "백서의 대망을 혈관경으로 이식하여 생성시킨 이차적 도서형 피판" 대한미세수술학회지 = Journal of Korean Society for Micorsurgery 5 (1): 16~23    
    2. 2000 "하인두 및 경부식도 결손의 재건 : 재건술의 선택" 대한 두경부 종양 학회지 = Korean journal of head & neck oncology 16 (1): 26~32    
    3. 2002 "재조합 인체 혈소판 성장인자(rh PDGF)를 이용한 만성 창상의 치료" 대한미세수술학회지 = Journal of Korean Society for Micorsurgery 11 (2): 173~178    
    4. 2004 "후 경골 동맥에서 분지한 비 특이성 비골 골피 유리 피판에서의 관통 혈류 미세 문합을 통한 피부판의 구제" 대한미세수술학회지 = Journal of Korean Society for Micorsurgery 13 (1): 24~28    
    5. 2004 "후구치삼각암의 수술적 치료" 대한 두경부 종양 학회지 = Korean journal of head & neck oncology 20 (2): 167~171    
    6. 2005 "광범위 종양절제술 후 발생한 하악 결손의 재건 : 결손부위에 따른 비골 유리 피판의 다양한 디자인" 대한 두경부 종양 학회지 = Korean journal of head & neck oncology 21 (2): 190~195    
    7. 2006 "소폭의 잔존 하인두벽을 이용한 첩포형 전완유리 피판 인두 재건술" 大韓成形外科學會誌 = Journal of the Korean society of plastic and reconstructive surgeons 33 (4): 407~412    
    8. 2006 "고령 환자군에 있어서 유리피판술을 이용한 두경부재건의 안전성" 大韓成形外科學會誌 = Journal of the Korean society of plastic and reconstructive surgeons 33 (3): 289~293    
    9. 2006 "중안면부 복합조직결손부의 재건을 위한 다양한 유리피판술의 선택" 大韓成形外科學會誌 = Journal of the Korean society of plastic and reconstructive surgeons 33 (3): 283~288    
    10. 2006 "켈로이드 절제술 후 방사선치료 효과에 대한 후향적 연구" 大韓成形外科學會誌 = Journal of the Korean society of plastic and reconstructive surgeons 33 (6): 706~710    
  • 홍종원 (14)

  • 탁관철 (48)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • 원문이 없습니다.
유료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기