본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

생명과학회지 = Journal of life science v.18 no.2 = no.94, 2008년, pp.220 - 227   피인용횟수: 14

번행초 추출물의 항산화 활성
Antioxidant Activities of the Solvent Extracts from Tetragonia tetragonioides

이민아   (창원대학교 대학원 생명공학협동과정UU0001233  ); 최혜정   (창원대학교 대학원 생명공학협동과정UU0001233  ); 강점순   (부산대학교 생명자원과학부UU0000613  ); 최영환   (부산대학교 생명자원과학부UU0000613  ); 주우홍   (창원대학교 생물학과UU0001233  );
  • 초록

    본 연구에서는 예로부터 위장병의 예방 및 치료 효과가 뛰어난 작물로 알려진 번행초(Tetragonia tetragonioides)의 각종 용매 추출 분획물의 항산화 효과를 명확히 하기 위해 총 폴리페놀과 플라보노이드 함량, 비타민 E함량, DPPH free radical 소거활성, Superoxide 음이온 소거활성, 과산화물생성 억제 활성, 환원능력 그리고 금속 킬레이트 효과를 측정하였다. 먼저 총 폴리페놀 화합물의 함량은 DCM 분획, EA 분획에서 높게 나타났고, 플라보노이드 함량은 HX 분획, EA 분획 순으로 나타났다. 비타민 E의 함량은 HX 분획, DCM 분획 순으로 높게 나타났다. DPPH radical 소거활성 효과는 DCM과 EA 추출 분획에서 $IC_{50}$ 값이 각각 $554.25{\mu}g/ml,\;394.96{\mu}g/ml$ 으로 산화방지제로 널리 이용되고 있는 BHT의 $784.7{\mu}g/ml$ 보다 우수하게 측정되었다. 과산화물 생성 억제 활성에서 번행초 분획은 지질단백질을 산화로부터 보호한다고 알려진 ${\alpha}$ -tocopherol과 유사한 능력을 보였으나, 페놀계 합성 항산화제인 BHT, BHA에 비해서는 억제능이 다소 낮음이 확인되었다. 환원능력을 통한 항산화 활성 측정에서는 1.5 mg/ml농도에서 EA 분획의 활성이 ${\alpha}$ -tocopherol보다 유의하게 높게 나타나 좋은 항산화 소재임이 확인되었다.

    To clarify the antioxidation effect of the various solvent fractionation of Tetragonia tetragoniodes which has been known to superior plants for the traditional prevention and treatment of stomach-related diseases, total polyphenol and flavonoid contents, vitamin E content, the elimination activity of a DPPH free radical and superoxide anion, inhibition activity of the superoxide generation, reducing power and metal chelating effects were estimated. The contents of the polyphenol compounds were highest in the DCM and EA fractionation, and the content of flavonoid was high in the order of HX and EA fractionation. The vitamin E content showed high in the order of the HX and DCM fractionation among solvent fractions. $IC_{50}$ for the elimination effect of a DPPH radical were estimated as 554.25 and $394.96{\mu}g/ml$ in the DCM and EA fractions, respectively. These values were higher than that of BHT $784.7{\mu}g/ml$ widely used in antioxidation effect. The inhibition activity of the superoxide generation using the T. tetragoniodes solvent fractions represented the effects similar to that of ${\alpha}$ -tocopherol known to prevent the lipoprotein oxidation, but lower consequences than that of the phenol-resins, BHT and BHA, respectively. In the antioxidation activity derived by the reducing capability, the EA fractionation in a 1.5 mg/ml concentration showed higher than that of ${\alpha}$ -tocopherol.

  • 주제어

    Tetragonia tetragonioides .   solvent extract .   antioxidant activity.  

  • 참고문헌 (30)

    1. Aruoma, O. I. 1994. Nutrition and health aspects of free radicals and antioxidants. Food Chem. Toxicol. 62, 671-683 
    2. Duh, P. D., Y. Y. Tu and G. C. Yen. 1999. Antioxidant activity of water extract of Harng Jyur (Chrysanthemum morifolium Ramat). Lebnesm. Wiss. Technol. 32, 269-277 
    3. Halliwell, B., J. M. C. Gutteridge and C. E. Cross. 1992. Free radicals, antioxidants and human disease: where are we now. J. Labratory and Clinical Medicine 119, 598-620 
    4. Kodato. S. I., M. Nakagawa and T. Hino. 1989. Synthesis of cerebroside B1b with antiulcerogenic activity II. Total synthesis and determination of absolute configuration of cerebroside B1b and its stereoisomers. Tetrahedron 45, 7263-7280 
    5. Lee. H. K., N. Y. Kim, M. J. Kim, S. U. Park and C. Y. Yu. 2003. Antioxidant, Antimutagenicity and Anticancer activities of extracts from Cirsium japonicum var. ussuriense KITAMURA. Korean J. Medicinal Crop. Sci. 11, 53-61 
    6. Lee, S. J., H. Y. Chung, I. K. Lee and I. D. Yoo. 1999. Isolation and identification of flavonoids from ethanol extracts of Artemisia vulgaris and their antioxitant activity. Korean J. Food Sci. Technol. 31, 815-822 
    7. Meir, S., J. Kanner, B. Akiri and S. P. Hadas. 1995. Determination and involvement of aqueous senescing leaves. J. Agric. Food Chem. 43, 1813-1815 
    8. Osawa, T., M. Namiki. 1981. A novel type of antioxidant isolate from leaf wax of Eucalyptus leaves. Agric. Biol. Chem. 45, 735-740 
    9. Woisky, R. G. and A. Salatino. 1998. Analysis of propolis: some parameters and procedures for chemical quality control. J. Apic. Res. 37, 99-105 
    10. Sato. T., F. Kiuchi and U. Sankawa. 1982. Inhibition of phenylalanine ammonia-lyase by cinnamic acid derivatives and related compounds. Phytochemistry 21, 845-850 
    11. Cambie, R. C and L. R. Ferguson. 2003. Potential functional foods in the traditional Maori diet. Fundam. Mol. Mecha. Mutag. 523-524, 109-117 
    12. Slinkard, K. and V. L. Singleton. 1997 Total phenol analyses: Automation and comparison with manual methods. Am. J. Enol. Vitic. 28, 49-55 
    13. Emi. O. and Y. Mikio. 1983. The principles of Tetragonia tetragonioides having anti-ulcergenic activity. Isolation and identification of a sterol glucoside mixture (compound A). Yakuguku zasshi 103, 43-48 
    14. Decker, E. A. and B. Welch. 1990. Role of ferritin as a lipid oxidation catalyst in muscle food. J. Agric. Food Chem. 38, 674-677 
    15. Ryu, H. Y., K. H. Bae, E. J. Kum, S. J. Park, B. H. Lee and H. Y. Sohn. 2007. Evaluation for the Antimicrobial, Antioxidant and Antithrombosis Activity of Natural Spices for Fresh-cut yam. J. Life Sci. 17, 652-657     
    16. Madavi, D. L. and D. K. Salunkhe. 1995. Toxicological aspects of food antioxidant. Food antioxidants pp. 267, Marcel Dekker., New York 
    17. Mori. K. and T. Kinsho. 1988. Synthesis of anti-ulcergenic cerebroside isolated Tetragonia tetragonioides. Liebigs. Ann. Chem. 807-814 
    18. Nishikimi, M., N. A. Rao and K. Yagi. 1972. The occurrence of superoxide anion in the reaction of reduced phenazine methosulphate and molecular oxygen. Biochem. Biophys. Res. Commun. 46, 849-864 
    19. Van Gelder, C. W. G., W. H. Flurkey and H. J. Wicher 1997. Sequence and structural features of plant and fungal tyrosinase. Phytochemistry 47, 1309-1323 
    20. Yoo, M. Y., S. H. Park, T. M. Kang and J. Y. Yang. 2005. Characterization of Antioxidants Extracted from Leaves of Sanjook (Sasa Boreails var. Chiisanensis). J. Life Sci. 15, 796-801     
    21. Bracke, M. E., H. T. Depyoere, T. Boterberg, E. Vanluchene, M. Nuytinck, R. Serreyn and M. M. Mareel. 1999. Influenece of tangeretion on tamoxifen's therapeutic benefit in mammary cancer. J. Nat. Can. Int. 91, 354-359 
    22. Hatano, T., R. Edamstsu, M. A. hiranatsu, Y. Fujita, T. Uhara, T. Yosyida and T. Okkuda. 1989. Effect of the inter-action of tannins with co-existing substances VI. Effects of tannins and related polyphenol superoxide anion radical and 1,1-diphenyl-2-picrylhydrazyl radical. Chem. Pharm. Bull. 37, 2016-2021 
    23. Prieto, P., M. Pineda and M. Aguilar. 1999. Spectophotometric Quantitaion of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E. Anal. Biochem. 269, 337-341 
    24. Cho, Y. J. 2005. Study on the tyrosinese activity inhibition and free radical scavenging activity of natural materials. pp. 58-62, University of Cyber Cosmetics Industry Sookmyung Women's, Korea 
    25. Aoki, T., K. Takagi, T. Hira and T. Suga. 1982. Two naturally occurring acyclic diterpene and norditerpene aldhydes from Tetragonia tetragonioides. Phytochemistry 21, 1361-1363 
    26. Kemo, M. S., R. S. Bueden and C. Brown. 1979. A new naturally occurring flavanone from Tetragonia expansa. Phytochemistry 18, 1765-1766 
    27. Bravo, L. 1998. Polyphenols: chemistry, dietary sources, metabolism and nutritional significance. Nutr. Rev. 56, 317-333 
    28. Oyaizu, M. 1986. Studies on products of browning reaction-Antioxidant activities of products of browning reaction prepared from glucoamine. Jpn. J. Nutritio. 44, 307-315 
    29. Singh, N. P. and P. R. Schmidt. 1989. Synthesis of a sphingadienine moiety containing cerebroside from tetragonia tetragonoides with antiulcerogenic activity. J. Carbohydr. Chem. 8, 199-216 
    30. Kato. M., T. Takeda, Y. Ogihara, M. Shimizu, T. Nomura and T. Tomita. 1985. Syudies on the structure of polysaccharide from Tetragonia tetragonoides. I. Chem. Pharm. Bull. 33, 3675-3680 
  • 이 논문을 인용한 문헌 (14)

    1. Choi, Hye-Jung ; Kang, Jum-Soon ; Choi, Young-Whan ; Jeong, Yong-Kee ; Joo, Woo-Hong 2008. "Inhibitory Activity on the Diabetes Related Enzymes of Tetragonia tetragonioides" 한국생물공학회지 = Korean journal of biotechnology and bioengineering, 23(5): 419~424     
    2. Kim, Yon-Suk ; Lee, Seung-Jae ; Hwang, Jin-Woo ; Kim, Ee-Hwa ; Park, Pyo-Jam ; Jeon, Byung-Tae 2011. "Antioxidant Activity and Protective Effects of Extracts from Helianthus tuberosus L. Leaves on t-BHP Induced Oxidative Stress in Chang Cells" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 40(11): 1525~1531     
    3. Hwang, Cho-Rong ; Hwang, In-Guk ; Kim, Hyun-Young ; Kang, Tae-Soo ; Kim, Yun-Bae ; Joo, Sung-Soo ; Lee, Jun-Soo ; Jeong, Heon-Sang 2011. "Antioxidant Component and Activity of Dropwort (Oenanthe javanica) Ethanol Extracts" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 40(2): 316~320     
    4. Lee, So-Mi ; You, Yang-Hee ; Kim, Kyung-Mi ; Park, Jeong-Jin ; Jeong, Chang-Sic ; Jhon, Deok-Young ; Jun, Woo-Jin 2012. "Antioxidant Activities of Native Gwangyang Rubus coreanus Miq." 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 41(3): 327~332     
    5. Kang, Hyun Woo ; Cho, Young-Jae 2013. "Antioxidant Activities of Extracts from Various Kimchi with Heat and Non-heat Treatment of Salt-Fermented Anchovies" 東아시아食生活學會誌 = Journal of the East Asian Society of Dietary Life, 23(2): 257~263     
    6. Kim, Min Jung ; Han, Young Sil 2014. "Antioxidant Activities of Cedrela sinensis Tender Leaf Powder Extracts obtained from Different Solvents" 한국식품영양학회지 = The Korean journal of food and nutrition, 27(6): 1059~1066     
    7. Kang, Jum-Soon ; Park, Eun-Ji ; Kim, So-Hee ; Heo, You ; Park, Young-Hoon ; Choi, Young-Whan ; Son, Beung-Gu ; Lim, Woo-Taik ; Suh, Jeong-Min 2014. "Effect of Seeds Treatment on Germinablity of Tetragonia Tetragonides Seeds" Journal of environmental science international = 한국환경과학회지, 23(5): 771~780     
    8. Choi, Hye Jung ; Yee, Sung-Tae ; Kwon, Gi-Seok ; Joo, Woo Hong 2015. "Anti-inflammatory and Anti-tumor Effects of Tetragonia tetragonoides Extracts" Microbiology and biotechnology letters = 한국미생물·생명공학회지, 43(4): 391~395     
    9. Lee, Sung-Gu ; Oh, Sung-Cheon ; Jang, Jae-Seon 2015. "Antioxidant Activities of Citrus unshiu Extracts obtained from Different Solvents" 한국식품영양학회지 = The Korean journal of food and nutrition, 28(3): 458~464     
    10. Choi, Eun Yeong ; Heo, Seong Il ; Kwon, Yong Soo ; Kim, Myong Jo 2016. "Anti-Oxidant Activity and Anti-Inflammatory Effects of Spiraea fritschiana Schneid Extract" 韓國藥用作物學會誌 = Korean journal of medicinal crop science, 24(1): 31~37     
    11. Jo, Jae-Bum ; Lee, Eun-Ho ; Cho, Young-Je 2016. "Beauty Food Activities of Isolated Phenolic Compounds from Tetragonia tetragonioides" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 45(3): 333~341     
    12. Jeong, Huijeong ; Kim, Hojun ; Ju, Eunshin ; Kong, Chang-Suk ; Seo, Youngwan 2016. "Antioxidant Effect of the Halophyte Atriplex gmelinii" KSBB Journal, 31(4): 200~207     
    13. 2016. "" Food science and biotechnology, 25(5): 1275~1281     
    14. Ha, Sung Min ; Choi, Hye Jung ; Shin, Gyeong Yeon ; Ryu, Beung Ho ; Joo, Woo Hong 2016. "Fermentation of Black Garlic Wine and its Characteristics" 생명과학회지 = Journal of life science, 26(7): 796~804     

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역



유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 이용한 콘텐츠
이 논문과 함께 출판된 논문 + 더보기