본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

녹차의 squalene synthase 저해효과
Inhibitory Effects of Green Tea against Squalene Synthase

최성원   (오산대학교 호텔조리계열UU0000992  ); 허남윤   (오산대학교 호텔조리계열UU0000992  ); 이한승   (신라대학교 바이오식품소재학과UU0000867  ); 백무열   (경희대학교 식품공학과UU0001575  ); 안순철   (부산대학교 의과대학 미생물학교실UU0000613  ); 이정규   (부산대학교 의과대학 가정의학과UU0000613  );
  • 초록

    콜레스테롤 생합성 과정에 있어서 속도조절 단계 효소의 하나인 squalene synthase에 대한 저해물질의 탐색을 목적으로, 30종의 다양한 천연물을 대상으로 squalene synthase에 대해 저해효과를 검토한 결과 녹차추출물에서 비교적 저해활성이 높고 재현성이 있게 저해효과를 나타내는 것으로 확인되었다. 녹차에 함유되어 있는 squalene synaase에 대한 저해물질의 용매추출성을 검토한 결과 ethyl acetate와 n-butanol 층에 저해물질이 많이 함유되어 있는 것으로 확인되었으며 저해물질은 녹차의 polyphenol 화합물인 catechin에 의한 것으로 추정되었다. Catechin 표준용액의 각 농도에 따른 squalene synthase 저해작용을 살펴 본 결과, (-)-epigallocatechin gallate, (-)-epicatechin gallate, (-)-epigauocatechin, (-)-epicatechin, (+)-catechin의 순으로 저해활성이 강한 것으로 나타났으며 가장 강한 저해활성을 나타내는 (-)-epigallocatechin gallate의 $IC_{50}$ 값은 $90{\mu}M$ 이었다.


    Various biological resources from plants, animals, mushrooms, microorganisms, and foods were tested for the inhibitory activity against squalene synthase (SQS). Among 32 samples, more than one fourths (9 samples) exhibited significant SQS inhibitory activity. Interestingly, SQS inhibitory activity was detected in the samples such as green tea, fermented soybean paste, and plum juice. The SQS inhibitory activity of green tea was not only high but also stable. Its SQS inhibitors were supposed to be catechin derivatives, which have been known to be main bioactive components in green tea. The galloyl catechins showed higher SQS inhibitory activity compared to the nongalloyl catechins. Especially, (-)-epigallocatechin gallate appeared to be strongest inhibitor against squalene synthase ( $IC_{50}=90{\mu}M$ ).


  • 주제어

    Squalene synthase .   inhibitors .   green tea .   catechin derivatives.  

  • 참고문헌 (32)

    1. Cohen, L. H., A. M. Griffioen and R. J. A. Wanders. 1986. Regulation of squalene synthase in rat liver. Biochem. Biophy. Res. Commu. 138, 335-341 
    2. Dugan, R. E. and J. W. Porter. 1972. Hog liver squalene synthase : The partial purification of the particulate enzyme and kinetic analysis of the reaction. Arch. Biochem. Biophy. 152, 28-35 
    3. Heider, J. G. 1986. Agent which inhibit cholesterol esterification in the intestine andtheir potential value in the treatment of hypercholesterolemia. J. R. Prous Science Publishers. pp. 423-438 
    4. Keller, R. K. and F. Vilsaint. 1993. Regulation of isoprenoid metaboliosm in rat liver: near constnat chain lengths of dolichy phosphate and ubiquinone are maintained during greatly altered rates of cholesterogenesis. Biochim. Biophy. Acta. 1170, 20-25 
    5. Feussner, G. 1994. HMG CoA reductase inhibitors. Curr. Opin. Lipidol. 5, 59-68 
    6. Stedronsky, E. R. 1994. Interaction of bile acids and cholesterol with non-systematic agents having hypocholesterolemic properties. Biochimica. et Biophysica. Acta. 1210, 255- 262 
    7. Tomada, H., H. Nishida, R. Masuda, J. Cao, S. Okuda and S. Omura. 1991. Purpactins, new inhibitors of acyl-CoA: cholesterol acyltransferase produced by Penicillium purpurogenum I. Production, isolation and physico-chemical and biological properties. J. Antibiotics 44, 136-145 
    8. Biller, S. A., C. Forster, E. M. Gordon, T. Harrity, W. A. Scott and P. Ciosek. 1988. Isoprenoid (phosphinylmethyl) phosphonates as inhibitors of squalene synthetase. J. Med. Chem. 31, 1869-1871 
    9. Hasumi, K., C. Shinohara, T. Iwanaga and A. Endo. 1993. Laterin, A new inhibitors of acyl-CoA: cholesterol acyltransferase produced by Gibberella lateritium IFO 7188. J. Antibiotics 46, 782-780 
    10. Park, J. K., K. Hasumi and A. Endo. 1993. Inhibitors of acyl- CoA: cholesterol acyltrans- ferase by Helminthosporol and its related compounds. J. Antibiotics 46, 1303-1310 
    11. Papjak, G., D. S. Goodman, J. W. Cornforth, R. H. Cornforth and R. Ryhage. 1961. Studies on the biosynthesis of cholesterol: X V. Mechanism of squalene biosynthesis from farnesyl pyrophosphate and from mevalonate. J. Biol. Chem. 236, 1934-1939 
    12. Agnew, W. S. 1985. Squalene synthetase. Methods in Enzymology. 110, 359-375 
    13. Bergstrom, J. D., M. M. Krutz, D. J. Rew, A. M. Amend, J. D. Karkas, R. D. Bostedor, V. S. Bansal, C. Dufresns, F. L. Van Middleworth, O. D. Hensens, J. M. Liesch, D. L. Zinc, K .E. Wilson, J. Onishi, J. A. Milligan, G. Bills, L. Kaplan, M. Nallin Omsted, R. G. Jenskins, L. Huang, M. S. Meinz, L. Quinn, R. U. Burg, Y. L. Kong, S. Mochales, M. Mojena, I. Martin, F. Pelaez, M. T. Diez and A. W. Albert. 1993. Zaragozic acids: A Family of metabolites that are picomolar competitive inhibitors of squalene synthase. Proc. Natl. Acad. Sci. USA 90, 80-89 
    14. Jin, H. H., J. L. Yang, J. H. Chung and Y. Kim. 2004. Hypocholesterolemic effects of green tea in cholesterol-fed rats. J. Korean Soc. Food Sci. Nutr. 33, 47-51     
    15. Shechter, I., I. Klinger and M. L. Rucher. 1992. Solubilization and characterization of a truncated form of rat hepatic squalene synthase. J. Biol. Chem. 267, 8628-8635 
    16. Biller, S. A., C. Forster, E. M. Gordon, T. Harrity, L. C. Rich, J. Marretta and P. Ciosek. 1991. Isoprenyl phosphi nylformates: new inhibitors of squalene synthetase. J. Med. Chem. 34, 1914-1916 
    17. Sidebottom, P. J., R. M. Highcock, S. J. Lane, P. A. Procopiou and N. S. Watson. 1992. The squalestatins, novel inhibitors of squalene synthase produced by a species of Phoma. II. Structure elucidation. J. Antibiotics 45, 648-658 
    18. Brown, M. S. and J. L. Goldstein. 1980. Multivalent feedback regulation of HMG-CoA reductase, a control mechanism coordinating isoprenoid synthesis and cell growth. J. Lipid Res. 21, 505-512 
    19. Dawson, M. J., J. E. Farthing, P. S. Marshall, R. F. Middleton, M. J. O'Neill, A. Shuttleworth, C. Stylli, R. M. Tait, P. M. Taylor, H. G. Wildman, A. D. Buss, D. Langley and M. V. Hayes. 1992. The squalestatins, novel inhibitors of squalene synthase produced by a species of Phoma. I. Toxonomy, fermentation, isolation, physico-chemical properties and biological activity. J. Antibiotics 45, 639-647 
    20. Poulter, C. D. and H. C. Rilling. 1991. Biosynthesis of isoprenoid compounds. pp. 413-441, Wiley, New York 
    21. Hertog, M. G., D. Kromhort, C. Aravanis, H. Blackburn, R. Buzina, F. Fidanza, S. Giampaoli, A. Jansen, A. Monotti and S. Nedeljkovic. 1995. Flavonoid intake and long-term risk of coronary heart diseases and cancer in the seven countries study. Arch. Int. Med. 155, 381-386 
    22. Spector, A. A. and M. A. Yorek. 1985. Membrane lipid composition and cellular function. J. Lipid Res. 26, 1015- 1021 
    23. Yamaguchi, Y., M. Hayashi, H. Yamazoe, M. Kunitomo. 1991. Preventive effects of green tea extract on lipid abnormalities in serum, liver and aorta of mice fed a atherogenic diet. Nippon Yakurigaku Zasshi 7, 329-337 
    24. Kuswik, R. G. and H. C. Rilling. 1987. Squalene synthetase. Solubilization and partial purification of squalene synthetase, copurification of presqualene pyrophosphate and squalene synthetase activities. J. Biol. Chem. 262, 1505-1510 
    25. Naganuma, S., Sakai, K. Hasumi and A. Endo. 1992. Acaterin, a novel inhibitor of acyl-CoA: cholesterol acyltransferase produced by Pseudomonas sp. A92. J. Antibiotics 45, 1216-1223 
    26. Padley, F. B. and J. Podmore. 1985. The role of fats in human nutrition. pp. 7-15, Ellis Horwood. Chichester 
    27. Tijburg, L. B., T. Mattern, J. D. Folts, U. M. Weigerber and M. B. Katen. 1997. Tea flavonoids and cardiovascular disease: a review. Crit. Rev. Food Sci. Nutr. 37, 771-785 
    28. Baxter, A., B. J. Fitzgerald, J. L. Hutson, A. D. McCarthy, J. M. Motteram, B. C. Ross, M. Sapra. M. A. Snowden, N. S. Watson, R. J. Williarm and C. Wright. 1992. Squalestatin I, a potent inhibitor of squalene synthase, which lower serum cholesterol in vivo. J. Biol. Chem. 267, 11705-11708 
    29. Goldstein, J. L. and S. M. Brown. 1990. Regulation of the mevalonate pathway. Nature 33, 425-433 
    30. Abe, I., J. C. Tomesch, S. Wattanasin and G. D. Prestwich. 1993. Inhibitors of squalene biosynthesis and metabolism. Natural Product Reports 11, 279-293 
    31. Park, C. O., S. H. Jin, and B. H. Ryu. 1996. Antioxidant activity of green tea extracts toward human low density lipoprotein. Korean J. Food Sci. Technol. 28, 850-858 
    32. Yeo, S. G., C. W. Lee, Y. W. Lee, T. G. Lee, Y. H. Park and S. B. Kim. 1995. Antioxidant effect of tea extract from green tea, Oolong tea and black tea. J. Korean Soc. Food Nutr. 24, 299-304 

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 이용한 콘텐츠
이 논문과 함께 출판된 논문 + 더보기