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대나무수액의 활성산소 소거활성과 세포독성
Reactive Oxygen Species and Cytotoxicity of Bamboo (Phyllostachys pubescens) Sap

조숙현   (경상남도농업기술원CC0138123  ); 최용조   (경상남도농업기술원CC0138123  ); 노치웅   (경상남도농업기술원CC0138123  ); 최철웅   (국제대학교 식품과학부UU0000256  ); 김덕송   (전남대학교 생물학과UU0001112  ); 조성환   (경상대학교 식품공학과 및 농업생명과학연구원UU0000114  );
  • 초록

    대나무수액의 자유라디칼 소거능을 측정하기 위하여 DPPH를 이용한 자유 라디칼 소거능 실험을 수행하여 항산화 효과를 측정한 결과 대나무수액의 농도가 높을수록 DPPH 활성이 뛰어남을 알 수 있었고, ROS를 이용하여 항산화효과를 확인하였다. 배양된 대식세포에 대나무수액을 농도별로 첨가한 결과 대나무수액 농토가 높을수록 과산화수소에 의해 유도된 산화적 자극이 감소하였다. 또한 세포 생존에 미치는 영향을 알고자 대나무수액을 농도별로 첨가하여 24시간 후 세포의 형태변화를 HIT assay로 실시한 결과 산화적 자극에 의해 발생한 세포손상이 대나무수액의 농도가 높아질수록 감소하는 것이 확인되었다. Ascorbic acid는 $H_2O_2$ 에 의해 야기되는 세포독성을 억제해 주는 효과가 10 %로 우수하게 나타났고 대나무수액 역시 고농도에서 27 %의 세포손상 방지효과가 우수하였다. 따라서 대나무수액은 안전하고 독성이 전혀 없는 천연 항산화제로서의 가능성을 보였다.


    The antioxidant properties of bamboos sap isolated from Phyllostachys pubescens were investigated. This product scavenged intracellular reactive oxygen species (ROS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and prevented lipid peroxidation. The radical scavenging activity of bamboo sap protected the viability of peritoneal macrophage cells exposed to hydrogen peroxide $(H_2O_2)$ , Furthermore, bamboo sap reduced apoptotic cell formation induced by $H_2O_2$ as demonstrated by decreases in the number of hypo-diploid cells am apoptotic cell body formation. These results indicate that bamboo sap has radical scavenging activity and ameliorates $H_2O_2$ induced cytotoxicity.


  • 주제어

    Bamboo sap .   ROS .   DPPH .   Lipid peroxidation .   antioxidant activity.  

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