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韓國營養學會誌 = The Korean journal of nutrition. v.41 no.1, 2008년, pp.22 - 30   피인용횟수: 2

사철쑥의 HeLa 세포고사 효과
Effects of Artemisia Capillaris Thunberg on Apoptosis in HeLa Cells

이형자   (한국식품공업협회 식품연구소CC0006761  ); 김기환   (원광대학교 식품영양학과UU0001021  ); 박종군   (원광대학교 생명과학부UU0001021  ); 황은희   (원광대학교 식품영양학과UU0001021  );
  • 초록

    사철쑥 (Artemisia capillaris Thunberg)이 암세포의 세포고사에 미치는 영향을 보기 위하여 인체 자국경부 상피암 세포주인 HeLa 세포를 이용하여 사철쑥의 메탄올, 에틸아세테이트, 부탄올 추출물의 농도를 각각 0, 100, 500, 1,000 및 $2,000{\mu}g/ml$ 로 처리하여 세포활성도, 세포형태 변화, DNA분절, DNA 함량을 알아보았다. 세포활성도는 사철쑥의 메탄올, 에틸아세테이트 추출물이 농도에 비례하여 HeLa세포생존율을 감소시켰지만 부탄올 추출물에서는 그 정도가 약하였고 물 추출물에서는 거의 관찰되지 않았다. 사철쑥의 메탄올, 에틸아세테이트, 부탄올과 물 추출물은 각 추출물의 농도에 의존적으로 배양된 HeLa 세포에 세포부착을 방해하고 세포막의 blobbing 및 핵의 분절화 등 세포고사의 특징들을 나타냈다. 사다리형 DNA 분절현상은 사철쑥의 메탄올 추출물 $1,000{\mu}g/ml$ , $2,000{\mu}g/ml$ 처리군에서 관찰되었다. 유세포분석기 (FACS)를 이용하여 측정한 고사세포의 DNA 함량은 메탄올과 에틸아세테이트, 부탄올 추출물이 농도 의존적으로 증가하였다. 이상의 결과는 사철쑥이 암세포의 세포고사를 증가시킴으로서 암 억제 작용이 있을 것을 시사해 준다고 할 수 있다.

    This study analyzes the apoptosis of HeLa cells to see if we can use the Artemisia capillaris Thunberg for the prevention of chronic degenerative diseases. We used the HeLa cells to see what effects the A. capillaris Thunberg had on apoptosis of the cancer cells. We checked the cell activity, cell morphological change, DNA fragmentation, and DNA content after administering 0, 100, 500, 1000, and $2000{\mu}g/ml$ methanol, ethyl acetate, n-butanol extract of the A. capillaris Thunberg. As for the cell viability, the increase of concentration of methanol and ethyl acetate decreased the survival rate of the cell, but the phenomenon was much weakened in n-butanol extract and was not observed in aqueous extract. The higher the density of the methanol, ethyl acetate, n-butanol and aqueous extract was, the lower the survival rate of the HeLa cell was. These extracts obstructed the cell cohesion and caused the blebbing of he cell membrane and fragmentation of the nucleus, both of which are symptoms of apoptosis. Laddering-pattern DNA fragmentation was observed in the groups that were treated with the $1000{\mu}g/ml$ and $2000{\mu}g/ml$ of methanol extract. The DNA content of the cells apoptosis measured by fluorescent-activated cell sorter (FACS) increased as the density of the methanol, ethyl acetate and butanol extract increased. The result of the study shows that A. capillaris Thunberg fosters the apoptosis of HeLa cells, which suggests that the A. capillaris Thunberg has a great potential value as food additives, medicinal supplements for patients with chronic diseases, and preventive measures against cancer.

  • 주제어

    Artemisia capillaris thunberg .   apoptosis .   HeLa cells.  

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  • 이 논문을 인용한 문헌 (2)

    1. Kim, In-Sook ; Park, Kwon-Sam ; Yu, Hyeon-Hee ; Shin, Mee-Kyung 2009. "Antioxidant Activities and Cell Viability against Cancer Cells of Adenophora remotiflora Leaves" 東아시아食生活學會誌 = Journal of the East Asian Society of Dietary Life, 19(3): 384~394     
    2. Kang, Kyoung-Myoung ; Lee, Shin-Ho 2013. "Effects of Extraction Methods on the Antioxidative Activity of Artemisia sp." 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 42(8): 1249~1254     

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