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Colchicine-Induced Polyploidy and It's Agronomic Characters in Bupleurum falcatum

손태권    ((주)이즈텍 기술연구소   ); 이상철    (경북대학교 농업생명과학대학   ); 정일경    (대구가톨릭대학교 생명공학과  );
  • 초록

    The effect of colchicine treatment on the agronomic performance and polyploid formation of Bupleurum falcatum using flow cytometry technique was investigated. The roots of 4-leaf stage plants were treated with colchicine (0.5%) for 3, 6, 12, and 24 hours and then transplanted in the field. Agronomic characters (survival rate, plant height, chlorophyll content, bolting rate) were recorded at 4 weeks and 6 months after transplanting while flow cytometry technique was conducted for determination of polyploid formation. Flow cytometry technique revealed polyploid nuclear DNA formation in colchicine treated plants. The highest number of polyploids was obtained at the shortest colchicine treatment time indicating an inverse relationship between colchicine treatment time and polyploid formation. Results also showed that survival and bolting rates were inversely related with the treatment time while plant height and chlorophyll were not significantly affected by the treatment. This study showed a convenient method for determination of colchicine-induced polyploid in B. falcatum and its superior agronomic performance at shorter treatment time.


  • 주제어

    Agronomic characters .   Bupleurum falcatum .   colchicine .   Flow cytometry .   Polyploid.  

  • 참고문헌 (29)

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