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Effect of Nelumbo nucifera on Proliferation, Migration and Expression of MMP-2 and MMP-9 of rSMC, A431 and MDA-MB-231

Karki, Rajendra   (Department of Medicinal Plant Resources, College of Natural Science, Mokpo National UniversityUU0000552  ); Rhyu, Dong-Young   (Department of Medicinal Plant Resources, College of Natural Science, Mokpo National UniversityUU0000552  ); Kim, Dong-Wook   (Department of Medicinal Plant Resources, College of Natural Science, Mokpo National UniversityUU0000552  );
  • 초록

    Nelumbo nucifera Gaertn.(family Nymphaeaceae) has been used for summer heat syndrome as home remedy in Japan, China and Korea. Although whole plant parts are edible, root is commonly consumed. It has been reported that rhizome extract showed anti-diabetic and anti-inflammatory effects. However, in spite of usefulness for treatment of various diseases, the effect of Nelumbo nucifera rhizome(NNR) on proliferation, migration and matrix degrading enzymes-matrix metalloproteinsases(MMPs), the expression of which degrades extracellular matrix(ECM) leading to metastasis, has not been fully elucidated. We examined the effect of hot water extract of NNR on the proliferation, migration and secretion of MMP-2 and MMP-9 in rat smooth muscle cells(rSMC), epidermoid cancer cells(A431) and breast cancer cells(MDA-MB-231). The proliferation assay was carried out using MTT assay, the principle of which depends upon the conversion of MTT by mitochondrial dehydrogensases of viable cells to formazan crystals. The effect of NNR on migration of cells was examined using wound healing assay. Our results showed that there was inhibition in the proliferation, migration and expression of MMP-2 and MMP-9 in dose dependent fashion in all the cells used. Thus, we concluded that NNR could be used as traditional medicine in the treatment of various diseases where proliferation, migration and MMPs' expression plays a pathological role like in restenosis and metastasis.


  • 주제어

    Nelumbo nucifera rhizome .   MMPs .   Proliferation .   Migration.  

  • 참고문헌 (25)

    1. Bell L. and J.A. Madri. 1989. Effect of platelet factors on migration of cultured bovine aortic endothelial and smooth muscle cells. Cir. Res. 65: 1057-1065 
    2. Fisher G.J. and J.J. Voorhees. 1998. Molecular mechanisms of photoaging and its prevention by retinoic acid: Ultraviolet irradiation induces MAP kinase signal transduction cascades that induce AP-1-regulated matrix metalloproteinases that degrade human skin in vivo. J. Investig Dermatol. Symp. Proc. 3: 61-68 
    3. Fisher G.J., S. Kang, J. Varani, Z. Bata-Csorgo, Y. Wan, S. Datta and J.J. Voorhees. 2002. Mechanisms of photoaging and chronological skin aging. Arch Dermatol 138: 1462-1470 
    4. Garbett E.A., M.W. Reed, T.J. Stephenson and N.J. Brown. 2000. Proteolysis in human breast cancer. Mol. Pathol. 53: 99-105 
    5. Hanemaaijer R., J.H. Verheijen, T.M. Maguire, H. Visser, K. Toet, E. McDermott, N. O'Higgins and M.J. Duffy. 2000. Increased gelatinase-A and gelatinase-B activities in malignant vs. benign breast tumors. Int. J. Cancer 86: 204-210 
    6. Iwata H., S. Kobayashi, H. Iwase, A. Masaoka, N. Fujimoto and Y. Okada. 1996. Production of matrix metalloproteinases and tissue inhibitors of metalloproteinases in human breast carcinomas. Jpn. J. Cancer Res. 87: 602-609 
    7. Kirtikar K.R. and B.D. Basu. 1975. Indian medicinal plants, Deharadun, India. pp. 116-120 
    8. Matsumura Y. and H.N. Ananthaswamy. 2002. Short-term and long-term cellular and molecular events following UV irradiation of skin: implications for molecular medicine. Exp. Rev. Mol. Med. 2002: 1-22 
    9. Woessner J.F. 1991. Matrix metalloproteinases and their inhibitors in connective tissue remodeling. FASEB J 5: 2145-2154 
    10. Bendeck M. P., N. Zempo, A.W. Clowes, R.E. Galardy and M.A. Reidy. 1994. Smooth muscle cell migration and matrix metalloproteinase expression after arterial injury in the rat. Circ Res. 75: 539-545 
    11. Kerkela E. and U. Saarialho-Kere. 2003. Matrix metalloproteinases in tumor progression: focus on basal and squamous cell skin cancer. Exp. Dermatol. 12: 109-125 
    12. Li X. and H. Wang. 2005. Chinese herbal medicine in the treatment of chronic kidney disease. Adv. Chronic Kidney Dis. 12(3): 276-281 
    13. Rittie L. and G.J. Fisher. 2002. UV-light-induced signal cascades and skin aging. Ageing Res. Rev. 1: 705-720 
    14. Fan Z., Y. Lu, X. Wu and J. Mendelsonn. 1994. Antibodyinduced epidermal growth factor receptor dimerization mediates inhibition of autocrine proliferation of A431 squamous carcinoma cells. J. Biol. Chem. 269: 27595-27602 
    15. Fischman D.L., M.B. Leon and D.S. Baim et al., 1994. A randomized comparison of coronary-stent placement and balloon angioplasty in the treatment of coronary artery disease. N. Engl. J. Med. 331: 496-501 
    16. John E.B., D.S. Edgar and E.A. Hubert. 2003. Matrix Metalloproteinase Expression in Breast Cancer. Journal of Surgical Research 110: 383-392 
    17. Kuzuya M., S. Kanda, T. Sasaki, N. Tamaya-Mori, X.W. Cheng, T. Itoh, S. Itohara and A. Iguchi. 2003. Deficiency of gelatinase a suppresses smooth muscle cell invasion and development of experimental intimal hyperplasia. Circulation. 108: 1375-1381 
    18. Kawamoto T., J.D. Sato, A. Le, J. Polikoff, G.H. Sato and J. Mendelsonn. 1983. Growth stimulation of A431 cells by epidermal growth factor: Identification of high-affinity receptors for epidermal growth factor by an anti-receptor monoclonal antibody. Proc. Natl. Acad. Sci. USA 80: 1337-1341 
    19. Curran S. and G.I. Murray. 2000. Matrix metalloproteinases: molecular aspects of their roles in tumour invasion and metastasis. Eur. J. Cancer 36: 1621 
    20. Mukherjee P.K., J. Das, K. Saha, S.N. Giri, M. Pal and B.P. Saha. 1996. Antipyretic activity of Nelumbo nucifera rhizome extract. Indian J. Exp. Biol. 34(3): 275-276 
    21. Fisher G.J., H.C. Choi and Z. Bata-Csorgo et al., 2001. Ultraviolet irradiation increases matrix metalloproteinase-8 protein in human skin in vivo. J. Invest Dermatol 117: 219-226 
    22. Mukherjee P.K., K. Saha, M. Pal and B.P. Saha. 1997. Effect of Nelumbo nucifera rhizome extract on blood sugar level in rats. Journal of Ethnopharmacology 58: 207-213 
    23. Jurkiewicz B.A. and G.R. Buettner. 1994. Ultraviolet light-induced free radical formation in skin: an electron paramagnetic resonance study. Photochem. Photobiol. 59: 1-4 
    24. Lindner V. and M.A. Reidy. 1991. Proliferation of smooth muscle cells after vascular injury is inhibited by an antibody against basic fibroblast growth factor. Proc. Natl. Acad. Sci. USA. 88: 3739-3743 
    25. Sander C.S., H. Chang, S. Salzmann, C.S. Muller, S. Ekanayake-Mudiyanselage, P. Elsner and J.J. Thiele. 2002. Photoaging is associated with protein oxidation in human skin in vivo. J. Invest. Dermatol. 118: 618-625 

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