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생약학회지 v.40 no.4 = no.159, 2009년, pp.370 - 375   피인용횟수: 8

5종 생약(진피, 청피, 귤핵, 지실, 지각)의 Flavanone Glycoside 함량분석과 Peroxynitrite 소거효과
Quantitative Analsysis of Flavanone Glycosides and Peroxynitrite Scavenging Effect of the Five Oriental Medicinal Drugs (Aurantii nobilis Pericarpium, Citrii unshiu Pericarpium, Citrii unshiu Semen, Aurantii Fructus, Poncirii Fructus)

누그로호 아궁    (상지대학교 제약공학과   ); 박명곤    (상지대학교 제약공학과   ); 진성은    (부경대학교 식품생명공학부   ); 최재수    (부경대학교 식품생명공학부   ); 박희준    (상지대학교 제약공학과  );
  • 초록

    Overproduction of peroxynitrite ( $ONOO^-$ ) causes a variety of disease such as atherosclerosis, hypercholesterolemia, diabetes mellitus or obesity. Peroxynitrite scavenging activities and HPLC analysis on the five Oriental medicinal drugs belonging to the genus Citrus, Aurantium or Poncirus (Rutaceae family) and HPLC analysis were taken to evaluate flavanone glycosides with peroxynitrite scavenging activity. The $IC_{50}s$ of the five crude drugs were shown as follows: Aurantii nobilis Pericarpium (Jinpi, 18.3 ${\mu}g$ /ml), Citrii unshiu Pericarpium (Chungpi, 7.50 ${\mu}g$ /ml), Citrii unshiu Semen (Gyulhaek, >50.0 ${\mu}g$ /ml), Aurantii Fructus (Jigak, 18.3 ${\mu}g$ /ml), and Poncirii Fructus (Jisil, >50.0 ${\mu}g$ /ml) where Korean crude drug's names are noted in the parenthesis. Peroxynitrite scavenging effect of flavanones or their glycosides usually contained in Citrus species were observed as follows: hesperetin (1.89 ${\mu}g$ /ml), naringenin (7.77 ${\mu}g$ /ml), hesperidin (8.44 ${\mu}g$ /ml), poncirin (>50.0 ${\mu}g$ /ml)and ponciretin(>50.0 ${\mu}g$ /ml). The activities of naringin and poncirin with ${\alpha}$ -L-rhamnopyranosyl( $1{\rightarrow}2$ )- ${\beta}$ -D-glucopyranosyl moiety were weak. HPLC analytical data revealed that Jinpi (the peels of mature fruits of Citrus unshiu) and Chungpi (the peels of immature fruits of C. unshiu) had high quantities of hesperidin as the value of 142.1 ${\pm}$ 0.21 and 104.51 ${\pm}$ 1.10 mg/g dried weight, respectively. Poncirin was clearly detected in only Jisil and naringenin and naringin were not observed on the HPLC chromatogram of the five crude drugs.


  • 주제어

    Citrus .   peroxynitrite .   flavanone glycosides .   hesperidin .   HPLC.  

  • 참고문헌 (21)

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    6. Park, Young-Chul ; Yang, Young-Tack ; Kim, Jin-Yeong ; Lee, Chang-Hoon ; Kang, Sang-Hoon ; Kang, Jong-Hoon 2015. "Characteristics of Flavonoids in Juice and Cluster Analysis of Satsuma Mandarin Germplasms" 韓國資源植物學會誌 = Korean journal of plant resources, 28(1): 16~25     
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