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Journal of the Korean Society for Applied Biological Chemistry v.52 no.5, 2009년, pp.525 - 530   SCIE 피인용횟수: 2
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Immunostimulatory Activity of Specific CpG Oligonucleotides from Bifidobacterium longum Genome on RAW 264.7 Macrophage Cells

Seo, Jeong-Min    (Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University   ); Choi, Young-Ok    (Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University   ); Ji, Geun-Eog    (Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University  );
  • 초록

    Bacterial CpG motifs have attracted considerable interests because of their immunomodulatory activities. Since Bifidobacterium is an important probiotic bacteria with characteristically high G+C content of 60 to 67%, investigation on the immune regulatory activity of CpG oligodeoxynucleotides (ODNs) from Bifidobacterium is warranted. The ODNs containing repeated TCG motifs were designed and synthesized based upon B. longum genomic sequence. Four 21-mer ODNs (ODNBL1~4) and three 25-mer ODNs (CpG ODN1~2, ODNBL5) induced macrophages to secrete cytokines such as IL-6, IL-12p40 and TNF- ${\alpha}$ . In particular, ODNBL5 induced macrophages to secret high amount of IL-6 and IL-12 compared with positive control PE2080 which was reported as a strong immune activating CpG ODN. This indicated that the specific functional sequences in the genome of B. longum could activate murine macrophage RAW 264.7 possibly acting as an immunostimulatory modulator for immune response.

  • 주제어

    Bifidobacterium longum .   CpG ODN .   immunoactivity .   macrophage cells.  

  • 참고문헌 (30)

    1. Branda RF, Moore AL, Hong R, McCormack JJ, Zon G, and Cunningham-Rundles C (1996a) B-cell proliferation and differentiation in common variable immunodeficiency patients produced by an antisense oligomer to the rev gene of HIV-1, Clin Immunol Immunopathol 79, 115-121 
    2. Branda RF, Moore AL, Lafayette AR, Mathews L, Hong R, Zon G, Brown T, and McCormack JJ (1996b), Amplification of antibody production by phosphorothioate oligodeoxynucleotides. J Lab Clin Med 128, 329-338 
    3. Hartmann G and Krieg AM (1999a) CpG DNA and LPS induce distinct patterns of activation in human monocytes, Gene Ther 6, 893-903 
    4. Jain VV, Kitagaki K, and Kline JN (2003) CpG DNA and immunotherapy of allergic airway diseases. A review, Clin Exp Allergy 33, 1330-1335 
    5. Kline JN, Krieg AM, Waldschmidt TJ, Ballas ZK, Jain V, and Businga TR (1999) CpG oligodeoxynucleotides do not require Th1 cytokines to prevent eosinophilic airway inflammation in a murine model of asthma, J Allergy Clin Immunol 104, 1258-1264 
    6. Locksley RM, Killeen N, and Lenardo MJ (2001) The TNF and TNF receptor superfamilies: integrating mammalian biology, Cell 104, 487-501 
    7. Takeshita F, Leifer CA, Gursel I, Ishii KJ, Takeshita S, Gursel M, and Klinman DM (2001) Cutting edge: role of Toll-like receptor 9 in CpG DNA-induced activation of human cells, J Immunol 167, 3555-3558 
    8. Krieg AM (1996) Lymphocyte activation by CpG dinucleotide motifs in prokaryotic DNA, Trends Microbiol 4, 73-76 
    9. Ferreira MA (2003), Cytokine expression in allergic inflammation: systematic review of in vivo challenge studies, Mediators Inflamm 12, 259-267 
    10. Ko EJ, Goh JS, Lee BJ, Choi SH, and Kim PH (1999) Bifidobacterium bifidum exhibits a lipopolysaccharide-like mitogenic activity for murine B lymphocytes, J Dairy Sci 82, 1869-1876 
    11. Kline JN (2007) Immunology of asthma using CpG oligodeoxynucleotides, Immunol Res 39, 279-286 
    12. Krieg AM (2002) A role for toll in autoimmunity, Nat Immunol 3, 423-424 
    13. Hartmann G and Krieg AM (2000) Mechanism and function of a newly identified CpG DNA motif in human primary B cells. J Immunol 164, 944-953 
    14. Kuribayashi K, Kodama T, Okamura H, Sugita M, and Matsuyama T (2002) Effects of post-inhalation treatment with interleukin-12 on airway hyper-reactivity, eosinophilia and interleukin-18 receptor expression in a mouse model of asthma, Clin Exp Allergy 32, 641-649 
    15. Branda RF, Moore AL, Mathews L, McCormack JJ, and Zon G (1993) Immune stimulation by an antisense oligomer complementary to the rev gene of HIV-1, Biochem Pharmacol 45, 2037-2043 
    16. Cohen J (1995) IL-12 deaths: explanation and a puzzle, Science 270, 908 
    17. Hoarau C, Lagaraine C, Martin L, Velge-Roussel F, and Lebranchu Y (2006) Supernatant of Bifidobacterium breve induces dendritic cell maturation, activation, and survival through a Toll-like receptor 2 pathway, J Allergy Clin Immunol 117, 696-702 
    18. Hartmann G, Weiner G, and Krieg AM (1999b) CpG DNA as a signal for growth, activation and maturation of human dendritic cells, Pro Natl Acad Sci USA 96, 9305-9310 
    19. Kitagaki K, Jain V, Businga T, Hussain I, and Kline JN (2002) Immunomodulatory effects of CpG oligodeoxynucleotides on established Th2 responses, Clin Diag Lab Immunol 9, 1260-1269 
    20. Seo JM, Ji GE, Cho SH, Park MS, and Lee HJ (2007), Characterization of a Bifidobacterium longum BORI dipeptidase belonging to the U34 family, Appl Environ Microbiol 73, 5598-5606 
    21. Hemmi H, Takeuchi O, Kawai T, Kaisho T, Sato S, Sanjo H, Matsumoto M, Hoshino K, Wagner H, Takeda K, and Akira S (2000) A Toll-like receptor recognizes bacterial DNA, Nature 408, 740-745 
    22. Menard S, Laharie D, Asensio C, Vidal-Martinez T, Candalh C, Rullier A, Zerbib F, Megraud F, Matysiak-Budnik T, and Heyman M (2005) Bifidobacterium breve and Streptococcus thermophilus secretion products enhance T helper 1 immune response and intestinal barrier in mice, Exp Biol Med (Maywood) 230, 749-756 
    23. Schell MA, Karmirantzou M, Snel B, Vilanova D, Berger B, Pessi G, Zwahlen MC, Desiere F, Bork P, Delley M, Pridmore RD, and Arigoni F. (2002) The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract, Proc Natl Acad Sci USA 99, 14422-14427 
    24. van der Poll T, Keogh CV, Guirao X, Buurman WA, Kopf M, and Lowry SF (1997), Interleukin-6 gene-deficient mice show impaired defense against pneumococcal pneumonia, J Infect Dis 176, 439-444 
    25. Jain VV, Kitagaki K, Businga TR, Hussain I, George C, O’shaughnessy P, and Kline JN (2002) CpG-oligodeoxynucleotides inhibit airway remodeling in a murine model of chronic asthma, J Allergy Clin Immunol 110, 867-872 
    26. Liang H, Nishioka Y, Reich CF, Pisetsky DS, and Lipsky PE (1996) Activation of human B cells by phosphorothioate oligodeoxynucleotides, J Clin Invest 98, 1119-1129 
    27. Krieg AM, Yi AK, Matson S, Waldschmidt TJ, Bishop GA, Teasdale R, Koretzky GA, and Klinmann DM (1995) CpG motif in bacterial DNA trigger direct B-cell activation, Nature 374, 546-549 
    28. Bryan SA, O’Connor BJ, Matti S, Leckie MJ, Kanabar V, Khan J, Warrington SJ, Renzetti L, Rames A, Bock JA, Boyce MJ, Hansel TT, Holgate ST, and Barnes (2000) Effects of recombinant human interleukin-12 on eosinophils, airway hyper-responsiveness, and the late asthmatic response, Lancet 356, 2149-2153 
    29. Kline JN, Waldschmidt TJ, Businga TR, Lemish JE, Weinstock JV, Thorne PS, and Krieg AM (1998) Modulation of airway inflammation by CpG oligodeoxynucleotides in a murine model of asthma, J Immunol 160, 2555- 2559 
    30. Li Y, Qu X, Yang H, Kang L, Xu Y, Bai B, and Song W(2005) Bifidobacteria DNA induces murine macrophages activation in vitro, Cell Mol Immunol 2, 473-478 
  • 이 논문을 인용한 문헌 (2)

    1. 2009. "" Journal of the Korean Society for Applied Biological Chemistry, 52(6): 716~719     
    2. 2013. "" Journal of the Korean Society for Applied Biological Chemistry, 56(1): 95~98     

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