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Journal of the Korean Society for Applied Biological Chemistry v.52 no.5, 2009년, pp.525 - 530   SCIE 피인용횟수: 2
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Immunostimulatory Activity of Specific CpG Oligonucleotides from Bifidobacterium longum Genome on RAW 264.7 Macrophage Cells

Seo, Jeong-Min    (Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University   ); Choi, Young-Ok    (Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University   ); Ji, Geun-Eog    (Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University  );
  • 초록

    Bacterial CpG motifs have attracted considerable interests because of their immunomodulatory activities. Since Bifidobacterium is an important probiotic bacteria with characteristically high G+C content of 60 to 67%, investigation on the immune regulatory activity of CpG oligodeoxynucleotides (ODNs) from Bifidobacterium is warranted. The ODNs containing repeated TCG motifs were designed and synthesized based upon B. longum genomic sequence. Four 21-mer ODNs (ODNBL1~4) and three 25-mer ODNs (CpG ODN1~2, ODNBL5) induced macrophages to secrete cytokines such as IL-6, IL-12p40 and TNF- ${\alpha}$ . In particular, ODNBL5 induced macrophages to secret high amount of IL-6 and IL-12 compared with positive control PE2080 which was reported as a strong immune activating CpG ODN. This indicated that the specific functional sequences in the genome of B. longum could activate murine macrophage RAW 264.7 possibly acting as an immunostimulatory modulator for immune response.


  • 주제어

    Bifidobacterium longum .   CpG ODN .   immunoactivity .   macrophage cells.  

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  • 이 논문을 인용한 문헌 (2)

    1. 2009. "" Journal of the Korean Society for Applied Biological Chemistry, 52(6): 716~719     
    2. 2013. "" Journal of the Korean Society for Applied Biological Chemistry, 56(1): 95~98     

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