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실크 피브로인 수화 겔의 형성에 미치는 티로시나아제 및 폴리페놀 화합물의 영향
Effect of Tyrosinase and Polyphenol Compounds on Hydrogelation of Silk Fibroin

박지훈    (충남대학교 바이오응용화학부 유기소재.섬유시스템   ); 정임    (충남대학교 바이오응용화학부 유기소재.섬유시스템   ); 박원호    (충남대학교 바이오응용화학부 유기소재.섬유시스템  );
  • 초록

    The formation of silk fibroin (SF) hydrogel can be adjusted by changing physical conditions such as concentration of SF aqueous solution, temperature, pH and salts. In this study, tyrosinase (Tyr), which is an enzyme catalyzing the oxidation of phenols such as tyrosine, was used to decrease the gelation time of SF aqueous solution under a fixed conditions. Tyr oxidizes a broad range of phenols into very reactive o-quinones, and consequently quinones undergo non-enzymatic reactions with various nucleophiles. So it is expected that the gelation time of SF aqueous solution could be decreased by polyphenol compound such as caffeic acid and chlorogenic acid. The color of SF aqueous solutions containing Tyr was changed into deeper yellow with Tyr concentration, and also the gelation time of SF aqueous solution slightly decreased. However, the effect of Tyr concentration on gelation time of SF aqueous solution was not significant due to the locational hindrance of tyrosyl residues in SF. Absorbance at 550 nm also showed conformational transition (random coil to $\beta$ -sheet conformation) of SF structure. When polyphenol compounds were added into SF/Tyr aqueous solution, the gelation time slightly decreased. However, the phase separation occurred when polyphenol compounds more than 5 mM were added. The results obtained in this study indicate that enzyme and additives have a potential to regulate the gelation behavior of SF aqueous solution, to some extent.


  • 주제어

    silk fibroin .   hydrogel .   gelation .   tyrosinase .   caffeic acid .   chlorogenic acid.  

  • 참고문헌 (27)

    1. C. Vepari and D. L. Kaplan, 'Silk as a Biomaterial', Prog Polym Sci, 2007, 32, 991-1007 
    2. S. Y. Seo, V. K. Sharma, and N. Sharma, 'Mushroom Tyrosinase: Recent Prospects', J Agric Food Chem, 2003, 51, 2837-2853 
    3. A. S. Ferrer, J. N. Rodriguez-Lopez, F. G. Canovas, and F. G. Carmona, 'Tyrosinase : A Comprehensive Review of Its Mechanism', Biochimica et Biophysica Acta 1995, 1247, 1-11 
    4. H. Yamamoto, S. Kuno, A. Nagai, A. Nishida, S. Yamauchi, and K. Ikeda, 'Insolubilizing and Adhesive Studies of Water-soluble Synthetic Model Proteins', Int J Biol Macromol, 1990, 12, 305-310 
    5. J. H. Whang and B. M. Lee, 'Inhibitory Effects of Plant Extracts on Tyrosinase, L-DOPA Oxidation, and Melanin Synthesis', Journal of Toxicology and Environmental Health-Part A-Current Issues, 2007, 70, 393-407 
    6. X. G. Li, L. Y. Wu, M. R. Huang, H. L. Shao, and X. C. Hu, 'Conformational Transition and Liquid Crystalline State of Regenerated Silk Fibroin', Biopolymers, 2008, 88, 497-505 
    7. B. S. Aytar and U. Bakir, 'Preparation of Cross-linked Tyrosinase Aggregates', Process Biochemistry, 2008, 43, 125-131 
    8. U. J. Kim, J. H. Park, C. Li, H. J. Jin, R. Valluzzi, and D. L. Kaplan, 'Structure and Properties of Silk Hydrogels', Biomacromolecules, 2004, 5, 786-792 
    9. K. Y. Lee and D. J. Mooney, 'Hydrogels for Tissue Engineering', Chem Rev, 2001, 101, 1869-1879 
    10. G. Wang, J. J. Xu, L. H. Ye, J. J. Zhu, and H. Y. Chen, 'Highly Sensitive Sensors Based on the Immobilization of Tyrosinase in Chitosan', Bioelectrochemistry, 2002, 57, 33-38 
    11. H. J. Jin and D. L. Kaplan, 'Mechanism of Silk Processing in Insects and Spiders', Nature, 2003, 424, 1057-1061 
    12. T. Kameda, Y. Ohkawa, K. Yoshizawa, E. Nakano, T. Hiraoki, A. S. Ulrich, and T. Asakura, 'Dynamics of the Tyrosine Side Chain in Bombyx mori and Samia cynthia ricini Silk Fibroin Studied by Solid State 2H NMR', Macromolecules, 1999, 32, 8491-8495 
    13. R. Nazarov, H. J. Jin, and D. L. Kaplan, 'Porous 3-D Scaffolds from Regenerated Silk Fibroin', Biomacromolecules, 2004, 5, 718-726 
    14. P. Monti, P. Taddei, G. Freddi, T. Asakura, and M. Tsukada, 'Raman Spectroscopic Characterization of Bombyx mori Silk Fibroin: Raman Spectrum of Silk I', Journal of Raman Spectroscopy, 2001, 32, 103-107 
    15. C. W. G. van Gelder, W. H. Flurkey, and H. J. Wichers, 'Sequence and Structural Features of Plant and Fungal Tyrosinases', Photochemistry, 1997, 45, 1309-1323 
    16. B. M. Min, L. Jeong, K. Y. Lee, and W. H. Park, 'Regenerated Silk Fibroin Nanofibers: Water Vapor-Induced Structural Changes and Their Effects on the Behavior of Normal Human Cells', Macromol Biosci, 2006, 6, 285-292 
    17. I. Gulcin, 'Antioxidant Activity of Caffeic Acid (3,4-dihydroxycinnamic acid)', Toxicology, 2006, 217, 213-220 
    18. J. A. Gerrard, S. E. Fayle, and K. H. Sutton, 'Covalent Protein Adduct Formation and Protein Cross-linking Resulting from the Maillard Reaction between Cyclotene and a Model Food Protein', J Agric Food Chem, 1999, 47, 1183-1188 
    19. A. Matsumoto, J. Chen, A. L. Collete, U. J. Kim, G. H. Altman, P. Cebeand, and D. L. Kaplan, 'Mechanism of Silk Fibroin Sol-Gel Transitions', J Phys Chem B, 2006, 110, 21630-21638 
    20. M. Y. Moridani, H. Scobie, A. Jamshidzadeh, P. Salehi, and P. J. O'brien, 'Caffeic Acid, Chlorogenic Acid, and Dihydrocaffeic Acid Metabolism: Glutathione Conjugate Formation', Drug Metabolism and Disposition, 2001, 29, 1432-1439 
    21. R. Valluzzi, S. Szela, P. Avtges, D. Kirschner, and D. L. Kaplan, 'Methionine Redox Controlled Crystallization of Biosynthetic Silk Spidroin', J Phys Chem B, 1999, 103, 11382-11392 
    22. V. S. Nithianandam and S. Erhan, 'Quinone-amine Polymers: 18. A Novel Method for the Synthesis of Poly(alkyl aminoquinone)s', Polymer, 1998, 39, 4095-4098 
    23. K. Komori, K. Yatagai, and T. Tatsuma, 'Activity Regulation of Tyrosinase by Using Photoisomerizable Inhibitors', J Biotech, 2004, 108, 11-16 
    24. T. Chen, H. D. Embree, L. Q. Wu, and G. F. Payne, 'In Vitro Protein-polysaccharide Conjugation: Tyrosinasecatalyzed Conjugation of Gelatin and Chitosan', Biopolymers, 2002, 64, 292-302 
    25. M. Yu, J. Y. Hwang, and T. J. Deming, 'Role of l-3,4-Dihydroxyphenylalanine in Mussel Adhesive Proteins', J Am Chem Soc, 1999, 121, 5825-5826 
    26. T. Asakura, K. Suita, T. Kameda, S. Afonin, and A. S. Ulrich, 'Structural Role of Tyrosine in Bombyx mori S ilk Fibroin, Studied by Solid-state NMR and Molecular Mechanics on Peptide Prepared as Silk I and II', Magnetic Resonance in Chemistry, 2004, 42, 258-266 
    27. G. D. Kang, K. H. Lee, C. S. Ki, and Y. H. Park, 'Crosslinking Reaction of Phenolic Side Chains in Silk Fibroin by Tyrosinase', Fibers Polym, 2004, 5, 234-238     

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