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Expression of Dengue Virus E Glycoprotein Domain III in Non-nicotine Transgenic Tobacco Plants

Kim, Mi-Young    (Division of Biological Sciences and the Research Center for Bioactive Materials, Chonbuk National University   ); Yang, Moon-Sik    (Division of Biological Sciences and the Research Center for Bioactive Materials, Chonbuk National University   ); Kim, Tae-Geum    (Division of Biological Sciences and the Research Center for Bioactive Materials, Chonbuk National University  );
  • 초록

    Dengue virus enters into host cells by binding envelope glycoprotein (E) to a receptor and causes human disease. Domain III of dengue virus E glycoprotein (EIII) is immunogenic and capable of inducing neutralizing antibodies. A DNA fragment encoding EIII (amino acids 297-394) was introduced into tobacco plant cells (Nicotiana tabacum L. cv MD609) by Agrobacterium tumefaciens-mediated transformation methods. The integration of EIII gene was observed in the genomic DNA of transgenic plants by PCR DNA amplification methods. The transcripts of EIII gene were detected in the transgenic plant leaf tissues by Northern blot analysis. The EIII protein was detected in transgenic plant leaf extracts by Western blot analysis. Based on the results of the Western blot analysis, the expression level of plant-produced EIII protein was between 0.13 and 0.25% of the total soluble protein in transgenic plant leaf tissues. The production of domain III of dengue virus E glycoprotein (EIII) in transgenic plants demonstrates the feasibility of using plant-based vaccines to prevent infection by the dengue virus.


  • 주제어

    dengue virus .   plant-based vaccine .   mucosal immunization .   domain III of dengue virus envelope glycoprotein.  

  • 참고문헌 (33)

    1. Mason, H. S., J. M. Ball, J. J. Shi, X. Jiang, M. K. Estes, and C. J. Arntzen (1996) Expression of norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice. Proc. Natl. Acad. Sci. USA 93: 5335-5340 
    2. Haq, T. A., H. S. Mason, J. D. Clements, and C. J. Arntzen (1995) Oral immunization with a recombinant bacterial antigen produced in transgenic plants. Science 268: 714-716 
    3. Mitchell, V. S., N. M. Philipose, and J. P. Sanford (1993) The children’s vaccine initiative. pp. 1-221. National Academy Press, Washington, DC, USA 
    4. Gubler, D. J. (2002) Epidemic dengue/dengue hemorrhagic fever as a public health, social, and economic problem in the 21st century. Trends Microbiol. 10: 100-103 
    5. Modis, Y., S. Ogata, D. Clements, and S. C. Harrison (2003) A ligand-binding pocket in the dengue virus envelope glycoprotein. Proc. Natl. Acad. Sci. USA 100: 6986-6991 
    6. Rey, F. A., F. X. Heinz, C. Mandi, C. Kunz, and S. G. Harrison (1995) The envelope glycoprotein from tickborne encephalitis virus at 2 ${\AA}$ resolution. Nature 375: 291-298 
    7. Van Haute, E., H. Joos, M. Maes, G. Warren, M. Van Montagu, and J. Schell (1983) Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of the Ti plasmids of Agrobacterium tumefaciens. EMBO J. 2: 411-417 
    8. Kang, T. J., S. C. Han, M. O. Jang, K. H. Kang, Y. S. Jang, and M. S. Yang (2004) Enhanced expression of Bsubunit of Escherichia coli heat-labile enterotoxin in tobacco by optimization of coding sequence. Appl. Biochem. Biotechnol. 117: 175-187 
    9. Kim,T. G., N. Befus, and W. H. Langridge (2004) Coimmunization with an HIV-1 tat transduction peptiderotavirus enterotoxin fusion protein stimulates a Th1 mucosal response in mice. Vaccine 22: 431-438 
    10. Suggrue, R. J., T. Cui, Q. Xu, J. Fu, and Y. C. Chan (1997) The production of recombinant dengue virus E protein using Escherichia coli and Pichia pastoris. J. Virol. Methods 69: 159-169 
    11. Wei, H. Y., L. F. Jiang, Y. H. Xue, D. Y. Fang, and H. Y. Guo (2003) Secreted expression of dengue virus type 2 full-length envelope glycoprotein in Pichia pastoris. J. Virol. Methods 109: 17-23 
    12. Bisht, H., D. A. Chugh, S. Swaminathan, and N. Khanna (2001) Expression and purification of dengue virus type 2 envelope protein as a fusion with hepatitis B surface antigen in Pichia pastoris. Protein Expr. Purif. 23: 84-96 
    13. Saejung, W., K. Fujiyama, T. Takasaki, M. Ito, K. Hori, P. Malasit, Y. Watanabe, I. Kurane, and T. Seki (2007) Production of dengue 2 envelope domain III in plant using TMV-based vector system. Vaccine 25: 6646-6654 
    14. Oszvald, M., T. J. Kang, S. Tomoskozi, C. Tamas, L. Tamas, T. G. Kim, and M. S. Yang (2007) Expression of a synthetic neutralizing epitope of porcine epidemic diarrhea virus fused with synthetic B subunit of Escherichia coli heat labile enterotoxin in rice endosperm. Mol. Biotechnol. 35: 215-223 
    15. Thanavala, Y., Y. F. Yang, P. Lyons, H. S. Mason, and C. J. Arntzen (1995) Immunogenicity of transgenic plant-derived hepatitis B surface antigen. Proc. Natl. Acad. Sci. USA 92: 3358-3361 
    16. Kelly, E. P., J. J. Greene, A. D. King, and B. L. Innis (2000) Purified dengue 2 virus envelope glycoprotein aggregates produced by baculovirus are immunogenic in mice. Vaccine 18: 2549-2559 
    17. Kim, T. G., N. X. Huy, M. Y. Kim, D. K. Jeong, Y. S. Jang, M. S. Yang, W. H. Langridge, and J. Y. Lee (2009) Immunogenicity of a cholera toxin B subunit Porphyromonas gingivalis fimbrial antigen fusion protein expressed in Escherichia coli. Mol. Biotechnol. 41: 157-164 
    18. Yu, D. H., M. N. Song, J. A. Lim, and D. I. Kim (2008) Effects of culture media on hCTLA4Ig production and protein expression patterns in transgenic rice cell suspension cultures. Biotechnol. Bioprocess Eng. 13: 424-430     
    19. Kim, T. G. and W. H. Langridge (2004) Synthesis of an HIV-1 Tat transduction domain-rotavirus enterotoxin fusion protein in transgenic potato. Plant Cell Rep. 22: 382-387 
    20. Kim, T. G. and W. H. R. Langridge (2003) Assembly of cholera toxin B subunit full-length rotavirus NSP4 fusion protein oligomers in transgenic potato. Plant Cell Rep. 21: 884-890 
    21. Roehrig, J. T., R. A. Bolin, and R. G. Kelly (1998) Monoclonal antibody mapping of the envelope glycoprotein of the dengue 2 virus, Jamaoca. Virology 246: 317-328 
    22. Mukhopadhyay, S., R. J. Kuhn, and M. G. Rossmann (2005) A structural perspective of the flavivirus life cycle. Nat. Rev. Microbiol. 3: 13-22 
    23. Cho, J. M., J. Y. Kwon, J. A. Lim, and D. I. Kim (2007) Increased hGM-CSF production and secretion with pluronic F-68 in transgenic Nicotiana tabacum suspension cell cultures. Biotechnol. Bioprocess Eng. 12: 594-600     
    24. Kozak, M. (1981) Possible role of flanking nucleotides in recognition of the AUG initiator codon by eukaryotic ribosomes. Nucleic Acids Res. 9: 5233-5252 
    25. De Cosa, B., W. Moar, S. B. Lee, M. Miller, and H. Daniell (2001) Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystals. Nat. Biotechnol. 19: 71-74 
    26. Marillonnet, S., C. Thoeringer, R. Kandzia, V. Klimyuk, and Y. Gleba (2005) Systemic Agrobacterium tumefaciens- mediated transfection of viral replicons for efficient transient expression in plants. Nat. Biotechnol. 23: 718-723 
    27. Crill, W. D. and J. T. Roehrig (2001) Monoclonal antibodies that bind to domain III of dengue virus E glycoprotein are the most efficient blockers of virus adsorption to Vero cells. J. Viol. 75: 7769-7773 
    28. Murashige, T. and F. Skoog (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473-497 
    29. Kapila, J., R. De Rycke, M. Van Montagu, and G. Angenon (1997) An Agrobacterium-mediated transient gene expression system for intact leaves. Plant Sci. 122: 101-108 
    30. Hurrelbrink, R. J. and P. C. McMinn (2003) Molecular determinants of virulence: the structural and functional basis for flavivirus attenuation. Adv. Virus Res. 60: 1-42 
    31. Maniatis, T., E. F. Fritsch, and J. Sambrook (1989) Molecular cloning. 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA 
    32. Arakawa, T., J. Yu, D. K. Chong, J. Hough, P. C. Engen, and W. H. Langridge (1998) A plant-based cholera toxin B subunit-insulin fusion protein protects against the development of autoimmune diabetes. Nat. Biotechnol. 16: 934-938 
    33. Yu, J. and W. H. Langridge (2000) Novel approaches to oral vaccines: delivery of antigens by edible plants. Curr. Infect. Dis. Rep. 2: 73-77 

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