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Biotechnology and bioprocess engineering v.14 no.5, 2009년, pp.559 - 564   SCI SCIE 피인용횟수: 1
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Production of $\beta$-carotene by Recombinant Escherichia coli with Engineered Whole Mevalonate Pathway in Batch and Fed-batch Cultures

Kim, Jung-Hun    (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University   ); Kim, Seon-Won    (Division of Applied Life Science (BK21), EB-NCRC and PMBBRC, Gyeongsang National University   ); Nguyen, Do Quynh Anh    (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University   ); Li, He    (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University   ); Kim, Sung-Bae    (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University   ); Seo, Yang-Gon    (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University   ); Yang, Jae-Kyung    (Division of Environmental Forest Science and Institute of Agriculture & Life Science, Gyeongsang National University   ); Chung, In-Young    (Department of Electronic and Communications Engineering, Kwangwoon University   ); Kim, Dae-Hwan    (School of Electrical Engineering, Kookmin University   ); Kim, Chang-Joon    (Department of Chemical & Biological Engineering and ERI, Gyeongsang National University  );
  • 초록

    Recombinant Escherichia coli engineered to contain the whole mevalonate pathway and foreign genes for $\beta$ -carotene biosynthesis, was utilized for production of $\beta$ -carotene in bioreactor cultures. Optimum culture conditions were established in batch and pH-stat fed-batch cultures to determine the optimal feeding strategy thereby improving production yield. The specific growth rate and volumetric productivity in batch cultures at $37^{\circ}C$ were 1.7-fold and 2-fold higher, respectively, than those at $28^{\circ}C$ . Glycerol was superior to glucose as a carbon source. Maximum $\beta$ -carotene production (titer of 663 mg/L and overall volumetric productivity of 24.6 mg/L $\times$ h) resulted from the simultaneous addition of 500 g/L glycerol and 50 g/L yeast extract in pH-stat fed-batch culture.


  • 주제어

    recombinant Escherichia coli .   engineered whole mevalonate pathway .   $\beta$-carotene .   bioreactor culture .   fed-batch culture.  

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  • 이 논문을 인용한 문헌 (1)

    1. Kim, You-Geun ; Lee, Young-Hoon ; Kang, Moon-Kook ; Lee, Byung-Hak ; Yun, Jun-Ki ; Kim, Sung-Bae ; Kim, Chang-Joon 2009. "Preparation of Functional Cosmetics Containing $\beta$-carotene Derived from Recombinant Escherichia coli and Evaluation of Anti-wrinkle Efficacy by Clinical Testing" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 37(4): 399~404     

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