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A New Formulated Stable Papin-pectin Aerosol Spray for Skin Wound Healing

Jauregui, Karla Monzerratt Gregorio    (Facultad de Ciencias Quimicas, Universidad Autonoma de Coahuila   ); Cabrera, Juan Carlos Cano    (Facultad de Ciencias Quimicas, Universidad Autonoma de Coahuila   ); Ceniceros, Elda Patricia Segura    (Facultad de Ciencias Quimicas, Universidad Autonoma de Coahuila   ); Hernandez, Jose Luis Martinez    (Facultad de Ciencias Quimicas, Universidad Autonoma de Coahuila   ); Ilyina, Anna    (Facultad de Ciencias Quimicas, Universidad Autonoma de Coahuila  );
  • 초록

    A new spray-on topical wound debrider composition consisting of 0.1% w/v papain immobilized in 6% w/v pectin gel was formulated for skin wound healing. The stability of the enzyme activity of this new formulated spray was compared with the 0.1% w/v papain in water solution at the refrigerated temperature of 4 and $75^{\circ}C$ . Two aerosol propellants, air and nitrogen, were evaluated for their efficacy. The new formulated spray was then evaluated for its thermal stability under air and $N_2$ at $75^{\circ}C$ . All enzyme activity of papain was measured with a spectrophotometer using casein as a substrate at $37^{\circ}C$ and in 0.1 M phosphate buffer at pH 6, as well as trichloral acetic acid to separate the product of casein hydrolysis. All the evaluations showed the new aerosol spray formulation to be far better than the liquid formulation in storage stability and thermal stability at $75^{\circ}C$ under atmospheric or higher pressures. The new formulation was tested in a rabbit experiment to evaluate the effectiveness of wound healing. As compared to untreated wound, the formulated spray clearly was superior, yielding a 20% more healing progress in the first 4 days alone.


  • 주제어

    papain stability .   pectin gel .   propellant pressure effect.  

  • 참고문헌 (23)

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