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Biotechnology and bioprocess engineering v.14 no.4, 2009년, pp.457 - 466   피인용횟수: 1

Extracellular $\beta$-glucosidase Production by a Marine Aspergillus sydowii BTMFS 55 under Solid State Fermentation Using Statistical Experimental Design

Madhu, K.M.    (Department of Biotechnology, Cochin University of Science and Technology   ); Beena, P.S.    (Department of Biotechnology, Cochin University of Science and Technology   ); Chandrasekaran, M.    (Department of Biotechnology, Cochin University of Science and Technology  );
  • 초록

    A potential fungal strain producing extracellular $\beta$ -glucosidase enzyme was isolated from sea water and identified as Aspergillus sydowii BTMFS 55 by a molecular approach based on 28S rDNA sequence homology which showed 93% identity with already reported sequences of Aspergillus sydowii in the GenBank. A sequential optimization strategy was used to enhance the production of $\beta$ -glucosidase under solid state fermentation (SSF) with wheat bran (WB) as the growth medium. The two-level Plackett-Burman (PB) design was implemented to screen medium components that influence $\beta$ -glucosidase production and among the 11 variables, moisture content, inoculums, and peptone were identified as the most significant factors for $\beta$ -glucosidase production. The enzyme was purified by $(NH_4)_2SO_4$ precipitation followed by ion exchange chromatography on DEAE sepharose. The enzyme was a monomeric protein with a molecular weight of ~95 kDa as determined by SDS-PAGE. It was optimally active at pH 5.0 and $50^{\circ}C$ . It showed high affinity towards eNPG and enzyme has a $K_m$ and $V_{max}$ of 0.67 mM and 83.3 U/mL, respectively. The enzyme was tolerant to glucose inhibition with a $K_i$ of 17 mM. Low concentration of alcohols (10%), especially ethanol, could activate the enzyme. A considerable level of ethanol could produce from wheat bran and rice straw after 48 and 24 h, respectively, with the help of Saccharomycescerevisiae in presence of cellulase and the purified $\beta$ -glucosidase of Aspergillussydowii BTMFS 55.


  • 주제어

    Aspergillus sydowii .   $\beta$-glucosidase .   Plackett-Burman .   SSF .   wheat bran.  

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  • 이 논문을 인용한 문헌 (1)

    1. Hemavathi, A.B. ; Raghavarao, K.S.M.S. 2011. "Membrane Processing for Purification and Concentration of ${\beta}$-glycosidases from Barley (Hordeum vulgare)" Biotechnology and bioprocess engineering, 16(2): 282~290     

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