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Biotechnology and bioprocess engineering v.14 no.4, 2009년, pp.474 - 481   피인용횟수: 1

Detection of Copper (II) in Aqueous Solution by Immobilized Urease Obtained from Agro-waste: Optimization of Process Variables

Talat, Mahe    (Department of Biochemistry, Faculty of Science, Banaras Hindu University  );
  • 초록

    Urease was used for estimation of $Cu^{2+}$ in aqueous solution. Process variables were optimized by Central Composite Design using MINITAB $^{(R)}$ 15 software. Results predicted by the design were close to experimental value indicating suitability of the model. 3D surface plot and isoresponse contour plot were helpful in predicting results by performing limited set of experiments. Urease was extracted from discarded seeds of pumpkin to apparent homogeneity by heat fractionation and gel filtration. Homogeneous enzyme preparation was further immobilized in 3.5% alginate. Effect of $Cu^{2+}$ on the activity of soluble and immobilized enzyme was investigated. Enzyme inhibition was biphasic, irreversible, and non-competitive (Ki = 1.06 ${\mu}M$ ).

  • 주제어

    Urease .   Alginate .   Immobilization .   Response Surface Methodology.  

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  • 이 논문을 인용한 문헌 (1)

    1. Lee, Min Hee ; Kim, Jong Kyu 2015. "Preparation and Adsorption-photocatalytic Activity Evaluation of TiO2-Coconut Shell Powder Composite (TCSPC)" 대한환경공학회지 = Journal of Korean Society of Environmental Engineers, 37(6): 357~362     

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