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Removal of $\alpha$-naphthol and Other Phenolic Compounds from Polluted Water by White Radish (Raphanus sativus) Peroxidase in the Presence of an Additive, Polyethylene Glycol

Ashraf, Humaira    (Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University   ); Husain, Qayyum    (Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University  );
  • 초록

    Role of white radish peroxidase has been investigated in the treatment of water contaminated with phenols, particularly $\alpha$ -naphthol. Water polluted with $\alpha$ -naphthol was treated with white radish peroxidase under various experimental conditions. The treatment of $\alpha$ -naphthol polluted water by this enzyme in presence of polyethylene glycol enhanced its removal. Studies carried out in absence of polyethylene glycol showed only 36% of $\alpha$ -naphthol removal however, 96% of it was removed in presence of 0.1 mg/mL of polyethylene glycol in 100 mM sodium phosphate buffer, pH 6.5, and 0.75 mM $H_2O_2$ at $40^{\circ}C$ . The other phenols oxidized and removed from waste water under similar experimental conditions were 18%, a-cresol; 30%, e-chlorophenol; 62%, e-bromophenol; 20%, benzyl alcohol; 21%, quinol; 38%, 2,6-dichlorophenol; 13%, 2,4-dichlorophenol; and 2%, native phenol. Mixtures of different phenolic compounds removed under identical treatment conditions were 63%, A; 40%, B; 52%, C; 41%, D; 72%, E; 66%, F; and 72%, G. Thus, peroxidase in presence of an additive, polyethylene glycol could be a suitable tool for the removal of phenolic compounds from industrial effluents.


  • 주제어

    peroxidase .   wastewater .   phenols .   polyethylene glycol.  

  • 참고문헌 (30)

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