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Enhancement of $\alpha$-ketoglutarate Production in Torulopsis glabrata: Redistribution of Carbon Flux from Pyruvate to $\alpha$-ketoglutarate

Zhang, Dandan    (The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University   ); Liang, Nan    (The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University   ); Shi, Zhongping    (The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University   ); Liu, Liming    (The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University   ); Chen, Jian    (The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University   ); Du, Guocheng    (The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University  );
  • 초록

    This manuscript aimed at increasing the production of $\alpha$ -ketoglutarate by the multi-vitamin auxotrophic yeast Torulopsis glabrata CCTCC M202019. The carbon flux was redistributed from pyruvate to $\alpha$ -ketoglutarate node by manipulating the specific activity of pyruvate dehydrogenase complex (PDH), pyruvate carboxylase (PC), pyruvate decarboxylase (PDC), and $\alpha$ -ketoglutarate dehydrogenase complex (KGDH). By proper increase of PDH, PC, and PDC activities, $\alpha$ -ketoglutarate in fermentation broth could be accumulated to the levels of 17.1 g/L, 21.6 g/L, and 31.2 g/L, respectively. In addition, decrease in the specific activity of KGDH also resulted in an enhanced $\alpha$ -ketoglutarate synthesis. With a proposed combinational enzymes regulation strategy, the highest $\alpha$ -ketoglutarate concentration of 37.7 g/L was achieved.


  • 주제어

    Torulopsis glabrata .   $\alpha$-ketoglutarata .   pyruvate.  

  • 참고문헌 (15)

    1. Chernyavskaya, O. G., N. V. Shishkanova, A. P. Il’chenko, and T. V. Finogenova (2000) Synthesis of alpha-ketoglutaric acid by Yarrowia lipolytica yeast grown on ethanol. Appl. Microbiol. Biotechnol. 53: 152-158 
    2. Liu, L. M., Y. Li, G. C. Du, and J. Chen (2003) $CaCO_3$ stimulates alpha-ketoglutarate accumulation during pyruvate fermentation by Torulopsis glabrata. Sheng Wu Gong Cheng Xue Bao, 19: 745-749 
    3. Tretter, L. and V. Adam-Vizi (2000) Inhibition of Krebs cycle enzymes by hydrogen peroxide: A key role of alpha-ketoglutarate dehydrogenase in limiting NADH production under oxidative stress. J. Neurosci. 20: 8972-8979 
    4. Applegate, M. A. B., K. M. Humphries, and L. I. Szweda (2008) Reversible inhibition of alpha-ketoglutarate dehydrogenase by hydrogen peroxide: Glutathionylation and protection of lipoic acid. Biochem. 47: 473-478 
    5. Reed, L. J. and B. B. Mukherjee (1969) $\alpha$-Ketoglutarate Dehydrogenase Complex from Escherichia coli. Methods Enzymol. 13: 55-61 
    6. Flikweert, M. T., L. VanderZanden, W. Janssen, H. Y. Steensma, J. P. VanDijken, and J. T. Pronk (1996) Pyruvate decarboxylase: An indispensable enzyme for growth of Saccharomyces cerevisiae on glucose. Yeast 12: 247-257 
    7. Stevenson, B. J., J. W. Liu, and D. L. Ollis (2008) Directed evolution of yeast pyruvate decarboxylase 1 for attenuated regulation and increased stability. Biochem. 47: 3013-3025 
    8. Kesheleva, N. A., L. S. Smirnova, and L. A. Baikova (1967) Biosynthesis of alpha-ketoglutaric acid in microorganisms. Izv Akad Nauk SSSR Biol. 1: 74-90 
    9. Tsugawa, R., T. Nakase, T. Kobayash, K. Yamashit, and S. Okumura (1969) Fermentation of N-Paraffins by Yeast. III. Alpha-Ketoglutarate Productivity of Various Yeast. Agric. Biol. Chem. 33: 929-941 
    10. Il’chenko, A. P., O. G. Chernyavskaya, N. V. Shishkanova, and T. V. Finogenova (2002) Metabolism of Yarrowia lipolytica grown on ethanol under conditions promoting the production of alpha-ketoglutaric and citric acids: A comparative study of the central metabolism enzymes. Microbiol. 71: 269-274 
    11. Hua, Q., C. Yang, and K. Shimizu (1999) Metabolic flux analysis for efficient pyruvate fermentation using vitamin-auxotrophic yeast of Torulopsis glabrata. J. Biosci. Bioeng. 87: 206-213 
    12. Finogenova, T. V., I. G. Morgunov, S. V. Kamzolova, and O. G. Chernyavskaya (2005) Organic acid production by the yeast Yarrowia lipolytica: A review of prospects. Appl. Biochem. Microbiol. 41: 418-425 
    13. Liu, L. M., Y. Li, H. Z. Li, and J. Chen (2004) Manipulating the pyruvate dehydrogenase bypass of a multivitamin auxotrophic yeast Torulopsis glabrata enhanced pyruvate production. Lett. Appl. Microbiol. 39: 199-206 
    14. Liang, N., M. Wang, and L. M. Liu (2008) Enhancing aketoglutaric acid production in Torulopsis glabrata: increasing of acetyl-CoA availability. Wei Sheng Wu Xue Bao 48: 874-878 
    15. Huang, H. J., L. M. Liu, Y. Li, G. C. Du, and J. Chen (2006) Redirecting carbon flux in Torulopsis glabrata from pyruvate to alpha-ketoglutaric acid by changing metabolic co-factors. Biotechnol. Lett. 28: 95-98 

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