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The journal of microbiology v.47 no.3, 2009년, pp.327 - 336  
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Mobilization Functions of the Bacteriocinogenic Plasmid pRJ6 of Staphylococcus aureus

Coelho, Marcus Livio Varella    (Departamento de Genetica, Instituto de Biologia, Instituto de Microbiologia   ); Ceotto, Hilana    (Departamento de Microbiologia Geral, Instituto de Microbiologia   ); Madureira, Danielle Jannuzzi    (Departamento de Microbiologia Geral, Instituto de Microbiologia   ); Nes, Ingolf F.    (UFRJ, Rio de Janeiro, Brasil, and Laboratory of Microbial Gene Technology, Norwegian University of Life Sciences   ); Bastos, Maria Do Carmo De Freire    (Departamento de Genetica, Instituto de Biologia, Instituto de Microbiologia  );
  • 초록

    Plasmid pRJ6 is the first known bacteriocinogenic mobilizable (Mob) plasmid of Staphylococcus aureus. Its Mob region is composed of four mob genes (mobCDAB) arranged as an operon, a genetic organization uncommon among S. aureus Mob plasmids. $oriT_{pRJ6}$ was detected in a region of 431 bp, positioned immediately upstream of mobC. This region, when cloned into pCN37, was able to confer mobilization to the recombinant plasmid only in the presence of pRJ6. The entire Mob region, including $oriT_{pRJ6}$ , is much more similar to Mob regions from several coagulase-negative staphylococci plasmids, although some remarkable similarities with S. aureus Mob plasmids can also be noted. These similarities include the presence within $oriT_{pRJ6}$ of the three mcb (MobC binding sites), firstly described in pC221 and pC223, an identical nick site also found in these same plasmids, and a nearly identical $sra_{pC223}$ site (sequence recognized by MobA). pRJ6 was successfully transferred to S. epidermidis by conjugation in the presence of the conjugative plasmid pGO1. Altogether these findings suggest that pRJ6 might have been originally a coagulase-negative staphylococci plasmid that had been transferred successfully to S. aureus.


  • 주제어

    mobilization .   bacteriocin .   oriT .   Staphylococcus .   plasmid pRJ6 .   conjugal transfer.  

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