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독도에 서식하는 가지과식물로부터 분리된 근권세균의 특성
Characterization of Rhizobacteria Isolated from Family Solanaceae Plants in Dokdo Island

함미선    (경북대학교 생명과학부, 경북대학교 울릉도.독도연구소   ); 박유미    (경북대학교 생명과학부, 경북대학교 울릉도.독도연구소   ); 성혜리    (경북대학교 생명과학부, 경북대학교 울릉도.독도연구소   ); Sumayo, Marilyn    (경북대학교 생명과학부, 경북대학교 울릉도.독도연구소   ); 류충민    (한국생명공학연구원 유전체연구센터   ); 박승환    (한국생명공학연구원 유전체연구센터   ); 김사열    (경북대학교 생명과학부, 경북대학교 울릉도.독도연구소  );
  • 초록

    독도에 자생하고 있는 야생 가지과 식물근권세균들의 특성을 밝히기 위해 까마중을 채집하였다. 까마중 근권으로부터 총 44종의 포자형성세균 혹은 질소고정세균을 분리하였다. 이 균주들은 16S rDNA 염기서열을 이용하여 부분 동정하였다. PGPR로써의 특성을 밝히기 위해 옥신 생산능, 인 가용능, 그리고 Siderophore 형성능을 측정하였다. 식물 생장호르몬인 옥신을 형성하는 균주는 19종, 난분해성 인을 분해할 수 있는 균주는 8종, 식물병원균을 억제하는 siderophore를 형성할 수 있는 균주는 13종으로 확인되었다. 우리나라에서 널리 재배되고 있는 가지과식물인 담배와 고추에 직접 적용하여, 식물의 생장 촉진 효과 및 식물 전신유도저항성 효과를 확인하였다. 특히 KUDC1009는 식물의 생장 촉진 효과 그리고 식물병원균에 대한 저항성을 증가시키는 등 다기능의 능력을 가지는 것을 확인할 수 있었다. 독도의 근권세균들은 다양한 스트레스 환경하에서 야생 까마중이 생존하는데 많은 도움을 주는 것을 추측 할 수 있었다.

    To characterize plant root-associated bacteria in wild plant family Solanaceae, Solanum nigrum L. plants were collected in Dokdo island. Forty four strains of nitrogen-fixing or spore-forming bacteria were isolated from rhizosphere of Solanum nigrum L. plants. Among these, 19 strains were able to produce auxin. Thirteen strains of these produced siderophore as determined by color reaction on CAS-blue plate, 8 strains were able to solubilize phosphate. The 16S rDNA genes of the isolated bacteria were amplified and sequenced. Model plants, pepper and tobacco, were established in order to evaluate the bacterial capacities eliciting growth promotion and induced systemic resistance. The plants treated with strain KUDC1009 were more resistant and capable of growth-promotion than control plants when challenged by either Xanthomonas axonopodis pv. vesicatoria or Erwinia carotovora sub. carotovora strain SCC1. Rhizobacteria isolated from Dokdo island can promote growth of wild type Solanum nigrum L. under much environmental stresses.

  • 주제어

    Dokdo .   Solanaceae .   plant growth-promoting rhizobacteria .   induced systemic resistance.  

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  • 이 논문을 인용한 문헌 (6)

    1. Jeon, Seon-Ae ; Sung, Hye-Ri ; Park, Yu-Mi ; Pak, Jae-Hong ; Ghim, Sa-Youl 2009. "Analysis of Endospore-forming Bacteria or Nitrogen-fixing Bacteria Community Isolated from Plants Rhizosphere in Dokdo Island" 한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, 37(3): 189~196     
    2. You, Young-Hyun ; Yoon, Hyeok-Jun ; Woo, Ju-Ri ; Seo, Yeong-Gyo ; Kim, Mi-Ae ; Choo, Yeon-Sik ; Kim, Jong-Guk 2011. "Plant Growth-Promoting Activity of Endophytic Fungi Isolated from the Roots of Native Plants in Dokdo Islands" 생명과학회지 = Journal of life science, 21(11): 1619~1624     
    3. You, Young-Hyun ; Yoon, Hyeok-Jun ; Lee, Gil-Seong ; Woo, Ju-Ri ; Rim, Soon-Ok ; Shin, Jae-Ho ; Lee, In-Jung ; Choo, Yeon-Sik ; Kim, Jong-Guk 2011. "Diversity and Plant Growth-Promotion of Endophytic Fungi Isolated from the Roots of Plants in Dokdo Islands" 생명과학회지 = Journal of life science, 21(7): 992~996     
    4. You, Young-Hyun ; Yoon, Hyeokjun ; Kim, Hyun ; Lim, Sung Hwan ; Shin, Jae-Ho ; Lee, In-Jung ; Choo, Yeon-Sik ; Kim, Jong-Guk 2013. "Plant Growth-Promoting Activity and Genetic Diversity of Endophytic Fungi Isolated from Native Plants in Dokdo Islands for Restoration of a Coastal Ecosystem" 생명과학회지 = Journal of life science, 23(1): 95~101     
    5. Kim, Ye-Eun ; Yoon, Hyeokjun ; You, Young-Hyun ; Kim, Hyun ; Seo, Yeonggyo ; Kim, Miae ; Woo, Ju-Ri ; Nam, Yoon-Jong ; Irina, Khalmuratova ; Lee, Gyeong-Min ; Song, Jin-Ha ; Jin, Young-Ju ; Kim, Jong-Guk ; Seu, Young-Bae 2014. "Diversity and Characteristics of Rhizosphere Microorganisms Isolated from the Soil around the Roots of Three Plants Native to the Dokdo Islands" 생명과학회지 = Journal of life science, 24(4): 461~466     
    6. You, Young-Hyun ; Park, Jong Myong ; Lim, Sung Hwan ; Kang, Sang-Mo ; Park, Jong-Han ; Lee, In-Jung ; Kim, Jong-Guk 2016. "Gibberellin A7 production by Aspergillus tubingensis YH103 and cultural characteristics of endophytic fungi isolated from Tetragonia tetragonoides in Dokdo islands" Korean journal of microbiology = 미생물학회지, 52(1): 32~39     

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