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조직 배양 모형에서 정상 구강 점막 상피에 대한 국소 유전자 요법
TOPICAL GENE DELIVERY TO NORMAL ORAL EPITHELIUM USING ADENOVIRUS IN ORGAN CULTURE MODEL

김태환    (한림대학교 의과대학 치과학교실   ); 곽명호    (한림대학교 의과대학 치과학교실   ); 이춘호    (한림대학교 의과대학 치과학교실   ); 박준우    (한림대학교 의과대학 치과학교실   ); 박영욱    (강릉원주대학교 치과대학 구강악안면외과학교실   ); 김성곤    (한림대학교 의과대학 치과학교실  );
  • 초록

    Background: Though it is clear that many types of viruses can infect the oral mucosa, its condition for infection is unclear. The purpose of this study was to analyze the conditions for viral infection of normal oral mucosa and explore the possibility of topical gene therapy to oral mucosa using a viral vector. Methods: Freshly taken fragments of the palate and the tongue of mice were used for organ culture. The specimens were exposed to green fluorescent protein (GFP)-adenoviral vector for 1 hour except for the control. Initial viral titer was $6.3{\times}10^{11}\;pfu/ml$ and the virus was diluted to working concentrations. The dilution ratio was 1:1,000 ( $6.3{\times}10^8\;pfu/ml$ ), 1:10,000 ( $6.3{\times}10^7\;pfu/ml$ ), and 1:100,000 ( $6.3{\times}10^6\;pfu/ml$ ). They were then cultured on a stainless steel wire mesh in an organ culture dish. The specimens were stereoscopically examined every 24 hours for 6 days, after which they were fixed and analyzed through immunohistochemical methods Results: There was no visible expression in the control, $6.3{\times}10^6\;pfu/ml$ , and $6.3{\times}10^7\;pfu/ml$ groups. Initial expression was observed at 24 hours after infection in both the palate and the tongue in $6.3{\times}10^8\;pfu/ml$ and the expression significantly increased until 3 days in the palate and 2 days in the tongue after infection (P


  • 주제어

    Viral infection .   GFP .   Adenovirus.  

  • 참고문헌 (19)

    1. da Silva LM, Guimaraes AL, Victoria JM et al : Herpes simplex virus type I shedding in the oral cavity of seropositive patients. Oral Dis 11 : 13, 2005 
    2. Berking C, Takemoto R, Satyamoorthy K et al : Basic fibroblast growth factor and UVB transform melanocytes in human skin. Am J Pathol 158 : 943, 2001 
    3. Chardonnet Y, Dales S : Early events in the interaction of adenoviruses with HeLa cells. I. Penetration of type 5 and intracellular release of the DNA genome. Virology 40 : 462, 1970 
    4. Wan H, Winton HL, Soeller C et al : Der p1 facilitates transepithelial allergen delivery by disruption of tight junctions. J Clin Invest 104 : 123, 1999 
    5. Nesbit M, Schaider H, Miller TH et al: Low-level monocyte chemoattractant protein-1 stimulation of monocytes leads to tumor formation in nontumorigenic melanoma cells. J Immunol 166 : 6483, 2001 
    6. Al-Bakkal G, Ficarra G, McNeill K et al : Human papilloma virus type 16 E6 gene expression in oral exophytic epithelial lesions as detected by in situ rtPCR. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 87 : 197, 1999 
    7. Foster TP, Rybachuk GV, Kousoulas KG : Expression of the enhanced green fluorescent protein by herpes simplex virus type 1 (HSV-1) as an in vitro or in vivo marker for virus entry and replication J Virol Methods 75 : 151, 1998 
    8. Lai CM, Lai YK, Rakoczy PE : Adenovirus and adenoassociated virus vectors. DNA Cell Biol 21 : 895, 2002 
    9. Clayman GL, Trapnell BC, Mittereder N et al : Transduction of normal and malignant oral epithelium by an adenovirus vector: the effect of dose and treatment time on transduction efficiency and tissue penetration. Cancer Gene Ther 2 : 105, 1995 
    10. Furlanetto DL, Crighton A, Topping GV : Differences in methodologies of measuring the prevalence of oral mucosal lesions in children and adolescents. Int J Paediatr Dent 16 : 31, 2006 
    11. Kim SG, Yang BE, Oh SH et al : The differential expression pattern of BMP-4 between the dentigerous cyst and the odontogenic keratocy. J Oral Pathol Med 34 : 178, 2005 
    12. Coyne CB, Kelly MM, Boucher RC et al : Enhanced epithelial gene transfer by modulation of tight junctions with sodium caprate. Am J Respir Cell Mol Biol 23 : 602, 2000 
    13. Yeudall WA : Human papilloma viruses and oral neoplasia. Oral Oncol Eur J Cancer 87 : 197, 1992 
    14. Kim SG, Chae CH, Cho BO et al : Apoptosis of oral epithelial cells in oral lichen planus caused by upregulation of BMP-4. J Oral Pathol Med 35 : 37, 2006 
    15. Eicher SA, Clayman GL, Liu TJ et al : Evaluation of topical gene therapy for head and neck squamous cell carcinoma in an organotypic model. Clin Cancer Res 2 : 1659, 1996 
    16. Lai CM, Lai YK, Rakoczy PE : Adenovirus and adenoassociated virus vectors. DNA Cell Biol 21 : 895, 2002 
    17. Walters RW, Grunst T, Bergelson JM et al : Basolateral localization of fiber receptors limits adenovirus infection from the apical surface of airway epithelia. J Biol Chem 274 : 10219, 1999 
    18. Miller CS : Herpes simplex virus and human papilloma virus infections of oral cavity. Semin Dermatol 13 : 108, 1994 
    19. Wickham TJ, Mathias P, Cheresh DA, et al: Integrins alpha v beta 3 and alpha v beta 5 promote adenovirus internalization but not virus attachment. Cell 73 : 309, 1993 

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