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미니돼지에서 자가 피부유래 전구세포와 탈회골 및 피브린 스케폴드를 이용한 하악골 골결손부의 골재생에 대한 연구
MANDIBULAR BONE REGENERATION USING AUTOGENOUS SKIN-DERIVED PRECURSOR CELLS WITH A MIXED DEMINERALIZED BONE AND FIBRIN GLUE SCAFFOLD IN MINIATURE PIGS

변준호    (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 경상대학교 건강과학연구원   ); 최문정    (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 경상대학교 건강과학연구원   ); 최영진    (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 경상대학교 건강과학연구원   ); 심경목    (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 경상대학교 건강과학연구원   ); 김욱규    (부산대학교 치의학전문대학원 구강악안면외과학교실   ); 김종렬    (부산대학교 치의학전문대학원 구강악안면외과학교실   ); 박봉욱    (경상대학교 의학전문대학원 치과학교실 구강악안면외과, 경상대학교 건강과학연구원  );
  • 초록

    Purpose: The aims of this study were to assess the in vitro co-culturing pattern of isolated skin-derived precursor cells (SKPs) with a mixed demineralized bone (DMB) and fibrin glue scaffold and to evaluate in vivo osteogenesis after transplantation of autogenous SKPs with a these mixed scaffold in the animal's mandibular defects. Materials and Methods: We isolated SKPs from the ears of adult 4 miniature pigs. The isolated SKPs were co-cultured with a mixed DMB and fibrin glue scaffold in a non-osteogenic medium for 1, 2, and 4 weeks. Histological characteristics of in vitro co-cultured cells and scaffold were evaluated. $1{\times}10^7\;cells/100\;{\mu}l$ of autogenous porcine SKPs were grafted into the mandibular defects with a DMB and fibrin glue scaffold. In the control sites, only a scaffold was grafted, without SKPs. After two animals each were euthanized at 2 and 4 weeks after grafting, the in vivo osteogenesis was evaluated with histolomorphometric and osteocalcin immunohistochemical studies. Results: Homogeneously shaped skin-derived cells were isolated from porcine ear skin after 3 or 4 weeks of primary culture. In vitro osteogenic differentiation of SKPs was observed after co-culturing with a DMB and fibrin glue scaffold in a non-osteogenic medium. Von Kossa-positive bone minerals were also noted in the co-cultured medium at 4 weeks. As the culture time progressed, the number of observable cells increased. Trabecular new bone formation and osteocalcin expression were more pronounced in the SKP-grafted group compared to the control group. Conclusion: These findings suggest that autogenous SKP grafting with a DMB and fibrin glue scaffold can serve as a useful alternative to bone grafting technique.


  • 주제어

    Skin-derived precursor cells .   In vitro osteogenesis .   In vivo osteogeneis .   Mandibular bone regeneration.  

  • 참고문헌 (34)

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  • 이 논문을 인용한 문헌 (1)

    1. Byun, June-Ho ; Kang, Eun-Ju ; Maeng, Geun-Ho ; Rho, Gyu-Jin ; Kang, Dong-Ho ; Lee, Jong-Sil ; Park, Bong-Wook 2010. "Maxillary sinus floor elevation using autogenous skin-derived mesenchymal stem cells in miniature pigs" 大韓口腔顎顔面外科學會誌 = Journal of the Korean Association of Oral and Maxillofacial Surgeons, 36(2): 87~93     

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