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구강점막 편평상피세포암에서 림프관형성 유전자 발현
GENE EXPRESSION FOR LYMPHANGIOGENIC FACTORS IN ORAL MUCOSAL SQUAMOUS CELL CARCINOMA

박영욱    (강릉원주대학교 치과대학 구강악안면외과학교실   ); 김성곤    (강릉원주대학교 치과대학 구강악안면외과학교실   ); 김소희    (강릉원주대학교 치과대학 약리학교실   ); 김한석    (울산의과대학교 강릉아산병원   ); 김민근    (국군 강릉병원 치과  );
  • 초록

    Background and Purpose: Vascular endothelial growth factor (VEGF)-C, VEGF-D and their tyrosine kinase receptor, VEGF receptor (VEGFR)-3 are recently known to have lymphangiogenic activities in various tumor types. Oral mucosal squamous cell carcinoma (OMSCC) easily metastasizes to cervical lymph nodes, so we determined the expression levels of VEGF-C, VEGF-D and VEGFR-3 in oral squamous cell carcinoma. Materials and Methods: We performed Western blot analyses with 4 OMSCC cultured tumor cell lines (SCC9, KB, YD-10B, YD-38), and with 7 surgical specimens of OMSCC for the detection of VEGF-C, VEGF-D and VEGFR-3 proteins. Expression of VEGF-C mRNA as well as mRNA for VEGFR-3 in 4 OMSCC cell lines (KB, SCC-4, SCC-9, YD-10B) was investigated by RT-PCR. We also measured VEGFC/VEGF-D protein concentrations in the media and protein concentration of VEGFR-3 in cell lysates of 4 OMSCC cell lines (SCC9, KB, YD-10B, YD-38) using commerical ELISA kits. Finally, we performed immunoprecipitation for the detection of VEGF-C in cell lysates of 4 OMSCC cells (KB, SCC-4, SCC-9, YD-10B) and real-time RT-PCR for the quantification of VEGF-C mRNA. Results: In the result of Western blotting with cell lysates of 4 OMSCC cells, we could not detect the protein expression of VEGF-C, VEGF-D, and VEGFR-3. But, all tumor tissues demonstrated VEGF-C and VEGFR-3. VEGF-C mRNA was detected at various levels in 4 OMSCC cell lines. Moreover, OMSCC cells secreted VEGF-C, not VEGF-D and VEGFR-3 was also detected in cell lysates of OMSCC by ELISA. Immunoprecipitation and real-time RT-PCR revealed VEGF-C was also expressed in 4 OMSCC cell lines. Conclusion: Taken together, tumor cells of OMSCC secrete VEGF-C, not VEGF-D. And VEGFR-3 is expressed tumor cells as well as OMSCC tumor tissues, needs further study.


  • 주제어

    VEGF-C .   VEGF-D .   VEGFR-3 .   Oral mucosl squmous cell carcinoma.  

  • 참고문헌 (30)

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  • 이 논문을 인용한 문헌 (3)

    1. Park, Young-Wook ; Kwon, Kwang-Jun ; Lee, Jong-Won 2010. "IMMUNOHISTOCHEMICAL STUDY ON EXPRESSION OF LYMPHANGIOGENIC FACTORS IN ORAL CANCER" 대한악안면성형재건외과학회지 = The journal of Korean association of maxillogacial plastic and reconstructive surgeons, 32(1): 1~8     
    2. Kye, Jun-Young ; Park, Young-Wook 2011. "Inhibition of Lymphatic Endothelial Growth Factor Receptor in a Murine Model of Oral Squamous Cell Carcinoma" 대한악안면성형재건외과학회지 = The journal of Korean association of maxillogacial plastic and reconstructive surgeons, 33(1): 1~9     
    3. Park, Young-Wook ; Cho, Ju-Won 2013. "Effect of Lymphangiogenic Factors on Survival in a Murine Model of Oral Squamous Cell Carcinoma" 대한악안면성형재건외과학회지 = The journal of Korean association of maxillogacial plastic and reconstructive surgeons, 35(1): 1~12     

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