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환경생물 = Korean journal of environmental biology v.27 no.2, 2009년, pp.140 - 145   피인용횟수: 3
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이온화 방사선 및 염화수은 처리에 따른 어류 간암세포의 생존능 평가
Synergistic Effects of Ionizing Radiation and Mercury Chloride on Cell Viability in Fish Hepatoma Cells

한민    (한국원자력연구원 방사선과학연구소   ); 현경만    (한국원자력연구원 방사선과학연구소   ); 모하마드닐리    (도네쉬방사선연구소   ); 황인영    (인제대학교 환경공학부   ); 김진규    (한국원자력연구원 방사선과학연구소  );
  • 초록

    All organisms are being exposed to harmful factors present in the environmental. The combined action of various factors is a distinguishing feature of modern life. An interaction between two chemicals is considered as synergistic when the effect produced is greater than the sum of the two single responses. The biological effects due to the combined action of ionizing radiation with the other factor are hard to estimate and predict in advance. In the current study, we investigated the synergistic effects between ionizing and $HgCl_2$ using fish hepatoma cells (PLHC-1 cells). The results showed a dramatic decrease of cell viability after simultaneous treatment of PLHC-1 cells with ionizing radiation and $HgCl_2$ . Neiither of the two had any cytotoxic effect when treated alone. The cytotoxicity of ionizing radiation was enhanced in the presence of $HgCl_2$ . The synergistic effects were observed after exposure of the PLHC-1 cells to ionizing radiation combined with $HgCl_2$ . The synergistic interaction was due to an increase of irreversibly damaged cells after the combined exposure. Analysis of the extent of synergistic interaction enables to make quantitative estimation of irreversibly damaged cells after the combined exposure. The present study suggests that PLHC-1 cells can serve as rapid screening tools for detecting the toxicity of harmful factors.


  • 주제어

    radiation .   mercury chloride .   fish hepatoma cells .   synergistic interaction.  

  • 참고문헌 (21)

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  • 이 논문을 인용한 문헌 (3)

    1. Ryu, Tae-Ho ; Nili, Mohammad ; An, Kwang-Guk ; Kim, Jin-Kyu 2010. "Evaluation of DNA Damage Induced by Mercury Chloride (II) and Ionizing Radiation in the Earthworm" 환경생물 = Korean journal of environmental biology, 28(4): 212~217     
    2. Ryu, Tae-Ho ; Nili, Mohammad ; An, Kwang-Guk ; Kim, Jin-Kyu 2011. "Influence of Mercury on the Repair of Ionizing Radiation-induced DNA Damage in Coelomocytes of Eisenia fetida" 환경생물 = Korean journal of environmental biology, 29(3): 236~240     
    3. Hwang, Un-Ki ; Ryu, Hyang-Mi ; Choi, Yong-Hwan ; Lee, Seung-Min ; Kang, Han-Seung 2011. "Effect of Cobalt (II) on the Fertilization and Embryo Development of the Sea Urchin ($Hemicentrotus$ $pulcherrimus$)" 환경생물 = Korean journal of environmental biology, 29(4): 251~257     

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