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환경생물 = Korean journal of environmental biology v.27 no.2, 2009년, pp.230 - 236  
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Protective Effect of Allomyrina dichotoma Larva Extract on tert-butyl Hydroperoxide-induced Oxidative Hepatotoxicity

Lee, Kyung-Jin    (Cell Dynamics Research Center, Department of life Science, Gwangju Institute of Science and Technology   ); Lee, Jong-Bin    (Department of Biological Science, College of Natural Sciences, Chonnam National University  );
  • 초록

    An extract of Allomyrina dichotoma larva (ADL), one of the insects used most frequently in traditional Chinese medicine for the treatment of liver diseases such as hepatocirrhosis and hepatofibrosis, was assessed for antioxidant bioactivity in this study. In the current work, we have investigated the protective effects of ADL extracts on tert-butyl hydroperoxide (t-BHP)-induced hepatotoxicity in cultured hepa1c1c7 cells and in the mouse liver. The treatment of the hepa1c1c7 cells with ADL extracts induced a significant reduction of t-BHP-induced oxidative injuries, as determined by cell cytotoxicity, lipid peroxidation (LPO) and reactive oxygen species contents, in a dose-dependent manner. Moreover, ADL extracts evidenced a protective effect against t-BHPinduced oxidative DNA damage, as revealed by the results of the Comet assay in hepa1c1c7 cells. ADL extracts also protected against hydroxyl radical-induced 2-deoxy-d-ribose degradation by ferric ion-nitrilotriacetic acid and $H_2O_2$ . In addition, ADL extracts were shown to be able to quench 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals. Our in vivo study revealed that ADL extracts pretreatment applied prior to t-BHP administration significantly prevented an increase in the serum levels of hepatic enzyme markers and reduced LPO in the mouse liver in a dose-dependent manner. Taken together, these results suggest that the protective effects of ADL extracts against t-BHP-induced hepatotoxicity may be attributable, at least in part, to its ability to scavenge free oxygen radicals, and to protect against DNA damage due to oxidative stress.


  • 주제어

    Allomyrina dichotoma larva .   tert-butyl hydroperoxide .   DNA damage .   oxidative stress.  

  • 참고문헌 (17)

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 저자의 다른 논문

  • Lee, Jong-Bin (33)

    1. 1987 "배양계배 근세포의 분화과정에 미치는 중금속 이온의 영향" 동물학회지 = The Korean journal of zoology 30 (4): 410~416    
    2. 1992 "국립공원(國立公園) 변산반도(邊山半島) 백천(白川)의 어류상(魚類相)에 관(關)하여" Korean journal of Ichthyology = 한국어류학회지 4 (2): 63~71    
    3. 1994 "동복호(同福湖)와 그 상류수계(上流水系)의 추(秋).동계(冬季) 어류상(魚類相)에 관한 연구(硏究)" Korean journal of Ichthyology = 한국어류학회지 6 (1): 28~38    
    4. 1996 "남조(藍藻) Microcystis aeruginosa Kutzing의 미세구조(微細構造)에 관(關)한 연구(硏究)" 한국전자현미경학회지 = Korean journal of electron microscopy 26 (4): 389~399    
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