본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

한국현미경학회지 = Korean journal of microscopy v.39 no.2, 2009년, pp.73 - 80   피인용횟수: 1
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

꽃피는식물 색소체 내 결정구조와 미세소관의 발달양상 조사 연구
A Survey of Plastid Crystals and Microtubules in Flowering Plants

김인선    (계명대학교 자연과학대학 생물학과  );
  • 초록

    다양한 색소체 내에 형성되는 결정체와 미세소관의 분포 및 발달양상을 식물이 수행하는 광합성 양식과 연계하여 꽃피는식물 종들에서 밝혀진 결과에 의하면, 색소체 결정체는 대개 격자 구조를 이루며 기질 또는 티라코이드 내에 형성되나, 미세소관은 비교적 규칙적인 형태로 기질에만 발달한다고 알려져 있다. 격자구조는 막으로 둘러싸이거나 막과는 무관하게 발달하며, 계통학적인 유연관계를 형성하지 않고 C-3 또는 C-4 또는 CAM 광합성을 수행하는 26개의 식물과, 약 56종에서 독립적으로 나타난다는 사실이 조사되었다. 색소체 결정체 및 미세소관 형성에 대한 지속적인 연구는 이들의 구조와 기능에 대하여 새로운 정보를 제공하나, 결정체에 대한 3차원적 입체구조 연구와 미세소관에 대한 분자생물학적인 실험법들이 요구되고 있는 실정이다. 특히, HVEM 고압전자현미경 및 tomography에 의한 연속절편 연구는 결정체의 3-D 입체구조 구현이 가능하여 심도 있는 구조정보를 제공하는 것으로 알려져 있다. 극히 일부의 식물에서만 보고되어 있는 색소체 내 미세소관에 대한 연구에 관해서는 먼저 색소체 기질에서 이들의 형성 시점을 알아내어 기질에서의 식별 및 분리 연구 등이 가능하게 돼야함이 강조되고 있다. 본 논문에서는 꽃피는식물 종들의 여러 색소체 유형에서 보고된 결정체와 미세소관을 조사하여 색소체 내에서 이들의 기능을 광합성과 연계하여 종합적으로 논의하였다.


    The plastid inclusion has long been known to exist in leaves of numerous plant species, especially in those of flowering plants. Among the inclusions, crystalline bodies are the most frequently distinguished structures of the foliar plastids, however, microtubules and phytoferritins are also reported occasionally. The crystalline inclusions vary in shape, and are located either in the stroma or within intrathylakoidal spaces, whereas microtubules and phytoferritins are more uniform in shape and are formed in the stroma. In crystalline structures, the composing elements exhibit a lattice pattern and/or paralleled tubules that are either bounded by membranes or exist without membrane enclosing. Other types of inclusions have not been shown to be enclosed by any membranous structures. According to the current survey, the plastid inclusion, with the exception of phytoferritins, has been shown to exhibit a crystalline or tubular pattern, and has been reported in more than 56 species of various families. Their occurrence is not restricted to any photosynthetic pathway, but is found to be randomly distributed among C-3, C-4 and CAM species, without phylogenetic relationships. The progress in plastid inclusion research reveals more information about the function and complexity, but the need for characterizing the 3-D structure of the crystalline inclusions also has been acknowledged in previous studies. A 3-D characterization would utilize tilting and tomography of serial sections with appropriate image processing that would provide valuable information on the sub-structures of the crystalline inclusions. In fact, recent studies performed on 3-D reconstruction of the plastid inclusions revealed important information about their comprising elements. In this article, the crystals and microtubules that have been reported in various types of plastids have been reviewed, with special consideration given to their possible sub-cellular function within the plastids.


  • 주제어

    Crystals .   Flowering plants .   Microtubules .   Plastid inclusions.  

  • 참고문헌 (75)

    1. Artus NN, Ryberg M, Sundqvist C: Plastid microtubule structures in wheat are insensitive to microtubule inhibitors. Physiol Plant 79 : 641-648, 1990 
    2. Casadoro G, Rascio N, Pagiusco M, Ravagnan N: Flowers of Orontium aquaticum L.: membrane rearrangement in chloroplastchromoplast interconversions. J Ultrastruct Res 81 : 202-208, 1982 
    3. Emter O, Falk H, Sitte P: Specific carotenoids and proteins as prerequisites for chromoplast tubule formation. Protoplasma 157 : 128-135, 1990 
    4. Gailhofer M, Kferbck T, Thaler I: Cytochemische Undersuchungen an Einschlssen in Plastiden der Bltter von Taraxacum officinale. Phyton 30 : 173-185, 1990 
    5. Gailhofer M, Thaler I: Eiweibkristalle in den Plastiden der Keimpflanzen einiger Palmen. Phyton 15 : 251-258, 1974 
    6. Hoefert LL, Esau K: Plastid inclusion in epidermal cells of Beta. Am J Bot 62 : 36-40, 1975 
    7. Hohl HR: Plastids and tumorigenesis. Am J Bot 48 : 528, 1961 
    8. Kim IS: Changes in the plastid ultrastructure during Sedum rotundifolium leaf development. J Plant Biol 49 : 376-383, 2006     
    9. Knoth R: Protein crystalloids in ribosome-deficient plastids of Aeonium domesticum cv. variegatum (Crassulaceae). Planta 156 : 528-535, 1982 
    10. Larsson C, Collin C, Albertsson P: The fine structure of chloroplast stroma crystal. J Ultrastruct Res 45 : 50-58, 1973 
    11. Lee KB: Ultrastructure of crystalline inclusion in the thylakoids of dodder (Cuscuta japonica) plastids. J Plant Biol 50 : 325-330, 2007b 
    12. Lyshede OB: Electron microscopy of the filiform seedling axis of Cuscuta pedicellata. Bot Gaz 150 : 230-238, 1989 
    13. Newcomb EH: Fine structure of protein-storing plastids in bean root tips. J Cell Biol 33 : 143-163, 1967 
    14. Price WC, Martinez AP, Warmke HE: Crystalline inclusions in chloroplasts of the coconut palm. J Ultrastruct Res 14 : 618-621, 1966 
    15. Rickson FR: The ultrastructure of Acasia cornigera L. Beltian body tissue. Amer J Bot 62 : 913-922, 1975 
    16. Rivera ER, Arnott HJ: Tubular structure in the plastids of Echinomastus intertextus Brit. & Rose. New Phytol 90 : 551-561, 1982 
    17. Ryberg H, Ryberg M, Sundqvist C: Plastid ultrastructure and development. In: Sundqvist, C Ryberg M. eds, Pigment-Protein Complexes in Plastids: synthesis and Assembly, pp. 25-62, Academic Press, San Diego, 1993 
    18. Santos I, Salema R: Chloroplast microtubules in some CAM-plants. Bol Soc Brot Ser (2) 53 : 1115-1122, 1981 
    19. Santos I, Salema R: Stereological study of the variation of the chloroplast tubules and volume in the CAM plant Sedum telephium. Z Pflanzenphysiol Bd 113 : 29-37, 1983 
    20. Shojima S, Nishizawa NK, Mori S: Do intrathylakoidal inclusions really contain RUBPCase? Protoplasma 140 : 187-189, 1987 
    21. Sitte P: Plastid metamorphosis and chromoplasts in Chrysosplenium. Z Pflanzenphysiol 73 : 243-265, 1974 
    22. Sprey B: Membranassoziierte Tubuli Wahrend der Chloroplastengenese von Hordeum vulgare L. Protoplasma 84 : 197-203, 1975 
    23. Thompson A, Vogel J, Lee RE: Carbon dioxide uptake in relation to a plastid inclusion body in the succulent Kalancho pinnata Persoon. J Exp Bot 28 : 1037-1041, 1977 
    24. Thomson WW, Journett RD: Observations on bodies in subsidiary cells of Opuntia sp. J Ultrastruct Res 31 : 439-443, 1970 
    25. Vanderzee D, Kennedy RA: Plastid development in seedlings of Echinochloa crus-galli var. oryzicola under anoxic germination conditions. Planta 155 : 1-7, 1982 
    26. Varkey PJ, Nadakavukaren MJ: Influence of leaf differentiation on the developmental pathway of Coleus chloroplasts. New Phytol 92 : 273-278, 1982 
    27. Wrischer M: Kristalloide im Plastidenstroma I. Elektronenmikroskopisch-cytochemische untersuchungen. Planta 75 : 309-318, 1967 
    28. Gailhofer M, Thaler I: 'Stromazentrum' in Leukoplasten der Epidermis von Asphodelus microcarpus. Phyton 19 : 97-102, 1978 
    29. Lee RE, Thompson A: The stromacentre of plastids of Kalancho Pinnata Persoon. J Ultrastruct Res 42 : 451-456, 1973 
    30. Pettifrew, WT, Vaughn KC: Physiological, structural, and immunological characterization of leaf and chloroplast development in cotton. Protoplasma 202 : 23-37, 1998 
    31. Sherman TD, Pettigrew WT, Vaughn KC: Structural and immunological characterization of the Cuscuta pentagona L. chloroplast. Plant Cell Physiol 40 : 592-603, 1999 
    32. Nisius A: The stromacentre in Avena plastids: An aggregation of $\beta$-glucosidase responsible for the activation of oat-leaf saponins. Planta 173 : 474-481, 1988 
    33. Suzuki S: Crystalline inclusion in epidermal and vascular plastids of the mature Amaranthus leaf. J Electron Microsc 27 : 141-143, 1978 
    34. Dustin P: Microtubules. Springer-Verlag, Berlin, pp. 94-126, 1984 
    35. Lee KB: Structure and development of the upper haustorium in the parasitic flowering plant Cuscuta japonica (Convolvulaceae). Amer J Bot 94 : 737-745, 2007 
    36. Willison JHM, Davey MR: Fraction 1 protein crystals in chloroplasts of isolated tobacco leaf protoplasts: a thin-section and freeze-etch morphological study. J Ultrastruct Res 55 : 303-311, 1976 
    37. Brinkley W: Microtubules: a brief historical perspective. J Struct Biol 118 : 84-86, 1997 
    38. Wrischer M: Protein crystalloids in the stroma of bean plastids. Protoplasma 77 : 141-150, 1973 
    39. Esau K: Crystalline inclusion in thylakoids of spinach chloroplasts. J Utrastruct Res 53 : 235-243, 1975 
    40. Lawrence ME, Possingham JV: Observations of microtubule structures within spinach plastids. Biol Cell 53 : 77-82, 1984 
    41. Nick P: Plant Microtubules: Potential for Biotechnology. Springer, Berlin, pp.1-50, 83-136, 2000 
    42. Vaughn KC, Coyle PC, Wilson KG: Modifications of plastid ultrastructure in an efficient yellow mutant of spinach. Photosynthetica 15 : 201-204, 1981 
    43. Dey PM, Brownleader MD, Harborne JB: The plant cell and its molecular components, In: Dey PM, Harborne JB, ed, Plant Biochemistry, pp. 1-48, Academic Press, 1997 
    44. Dodge JD, Lawes GB: Plastid ultrastructure in some parasitic and semi-parasitic plants. Cytobilogie 9 : 1-9, 1974 
    45. Platt-Aloia KA, Thomson WW: Membrane bound inclusions in epidermal plastids of developing sesame leaves and cotyledons. New Pytol 83 : 793-799, 1979 
    46. Sprey B: Intrathylakoid occurrence of ribulose 1,5-diphosphate carboxylase in spinach chloroplasts. Z Pflanzenphysiol 78 : 85-89, 1976 
    47. Rascio N, Colombo PM, Vicca FD, Chitano P: Intrathylakoidal crystal appearance during the vital cycle of spinach chloroplasts. Protoplasma 126 : 153-157, 1985 
    48. Gunning BES, Steer MW: Plant Cell Biology: Structure and Function. Jones and Bartlett Publishers, Boston, pp. 1-60, 1996 
    49. Laudi G, Medeghini Bonatti P, Fricano G: Ultrastructure of plastids of parasitic higher plants. V. Influence of light on Cuscuta plastids. Isr J Bot 23 : 145-150, 1974 
    50. Miller KR, Bloodgood RA, Staehelin LAL: Crystals within thylakoids: a structural analysis. J Ultrastruct Res 54 : 29-36, 1976 
    51. Kim IS, Pak JH, Seo SB, Song SD: Foliar ultrastructure of Korean Orostachys species. Korean J Electron Microscopy 25 : 457-463, 1995 
    52. Kuhn H: Chemismus, Struktur und Entstehung der Carotinkristllchen in der Nebenkrone von Narcissus poeticus L. var. 'La Riante'. J Ultrastruct Res 33 : 332-355, 1970 
    53. Ames IH: The fine structure of genetic tumor cells. Amer J Bot 59 : 341-345, 1972 
    54. Shumway LK, Weier TE, Stocking CR: Crystalline structures in Vicia faba chloroplasts. Planta 76 : 182-189, 1967 
    55. Ames IH, Pivorun JP: A cytochemical investigation of a chloroplast inclusion. Amer J Bot 61 : 794-797, 1974 
    56. McDonald MS: Photobiology of Higher Plants. John Wiley & Sons Ltd., San Fransisco, pp. 33-73, 149-198, 2003 
    57. Gailhofer M: Die Feinstruktur der Plastideneinschlsse von Ranunculus bulbosus. Phyton 23 : 197-210, 1983 
    58. Ljubesic N: Chromoplasts in the petals of Liriodendron tulipifera L. Z Pflanzenphysiol 91 : 49-52, 1979 
    59. Newcomb W: Plastid structure and development. In: Dennis DT, Turpin DH, Lefebvre DD, Layzell DB, eds, Plant Metabolism, pp. 255-259, Longman, Harlow, 1997 
    60. Galatis B, Apostolakos P: Microtubule organization and morphogenesis of stomata in caffeine-affected seedlings of Zea mays. Protoplasma 165 : 11-26, 1991 
    61. Oross JW, Possingham JV: Tubular structures in developing plastids of three dicotyledonous species. Can J Bot 69 : 136-139, 1991 
    62. Brandao I, Salema R: Microtubules in chloroplasts of a higher plant (Sedum sp.). J Submicr Cytol 6 : 381-390, 1974 
    63. Diaz-Ruiz JR: A Highly ordered protein from Pelargonium: structure and cellular localization. J Utrastruct Res 53 : 227-234, 1975 
    64. Kim IS: Chloroplast microtubules in young leaves of Sedum rotundifolium. J Plant Biol 40 : 115-119, 1997 
    65. Kim IS, Pak JH, Seo SB, Song SD: Ultrastructure of leaves in C$_{4}$ Cyperus iria and C$_{3}$ Carex siderosticta. J Plant Biol 42 : 213-221, 1999 
    66. Kim IS, Park SC, Han SS, Kim ES: Three-dimensional analysis of the mesophyll plastids using ultra high voltage electron micro-scopy. Korean J Electron Microscopy 36 : 217-226, 2006     
    67. Kim IS, Fisher DG: Structure aspects of the leaves of seven species of Portulaca growing in Hawaii. Can J Bot 68 : 1803-1811, 1990 
    68. Spurr AR, Harris WM: Ultrastructure of chloroplasts and chromoplasts in Capsicum annumI. Thylakoid membrane changes during fruit ripening. Amer J Bot 55 : 1210-1224, 1968 
    69. Vaughn KC, Wilson KG: Improved visualization of plastid fine structure: plastid microtubules. Protoplasma 108 : 21-27, 1981 
    70. Williams E: Fine structure of vascular and epidermal plastids of the mature maize leaf. Protoplasma 79 : 395-400, 1974 
    71. Lee KB: Ultrastructure and development of seedling of the parasitic weed Cuscuta japonica. J Plant Biol 50 : 213-219, 2007a 
    72. Salema R, Brandao I: Development of microtubules in chloroplasts of two halophytes forced to follow Crassulacean acid metabolism. J Ultrastruct Res 62 : 132-136, 1978 
    73. Vapaavuori EM, Korpilahti E, Nurmi AH: Photosynthetic rate in willow leaves during water stress and changes in the chloroplast ultrastructure with special reference to crystal inclusions. J Exp Bot 35 : 306-321, 1984 
    74. Finer JJ, Smith RH: Structure and development of plastids in epidermal cells of African violet (Saintpaulia ionantha Wendl.) in culture. Ann Bot 51 : 691-695, 1983 
    75. Mikulska E, Damsz B, Zolnierowicz H: Structural and functional polymorphism of plastids in leaves of Clivia miniata Rgl. I. Ontogenesis of plastids in epidermis and guard cells. Acta Soc Bot Pol 50 : 381-389, 1981 
  • 이 논문을 인용한 문헌 (1)

    1. Kim, In-Sun 2011. "Occurrence of Nuclear Inclusions in Plant Cells" 한국현미경학회지 = Korean journal of microscopy, 41(4): 229~234     

 저자의 다른 논문

  • 김인선 (49)

    1. 1994 "Dubautia속(屬) 3종(種) 식물(植物)의 엽육조직(葉肉組織) 미세구조(微細構造) 비교연구(比較硏究)" 한국전자현미경학회지 = Korean journal of electron microscopy 24 (4): 13~31    
    2. 1995 "무궁화의 화분형성 및 화분벽의 분화발달" Journal of plant biology = 식물학회지 38 (1): 95~105    
    3. 1995 "한국산(韓國産) 바위솔속(屬) 엽육조직(葉肉組織)의 미세구조(微細構造)" 한국전자현미경학회지 = Korean journal of electron microscopy 25 (4): 52~61    
    4. 1996 "Karyological Variation of Callus-derived Regenerants in Allium victorialis var. platyphyllum" Journal of plant biology = 식물학회지 39 (4): 231~235    
    5. 1996 "다육질성 둥근바위솔 수분저장세포의 특성" 한국전자현미경학회지 = Korean journal of electron microscopy 26 (4): 457~463    
    6. 1997 "Ultrastructural Aspects of Kranz Anatomy in Digitaria sanguinalis and Setaria viridis (Poaceae)" Journal of plant biology = 식물학회지 40 (2): 102~109    
    7. 1997 "Chloroplast Microtubules in Young Leaves of Sedum rotundifolium" Journal of plant biology = 식물학회지 40 (2): 115~119    
    8. 1998 "산성비가 전동싸리의 생장과 질소 고정 활성에 미치는 영향" 한국생태학회지 = The Korean journal of ecology 21 (1): 65~72    
    9. 1998 "Ultrastructural Aspects of Leaves in Festuca ovina and Poa sphondylodes (C-3 Poaceae)" Journal of plant biology = 식물학회지 41 (3): 170~177    
    10. 1999 "Ultrastructure of Leaves in $C_4$ Cyperus iria and $C_3$ Carex siderosticta" Journal of plant biology = 식물학회지 42 (3): 213~221    

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기