본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Ocean science journal : OSJ v.44 no.4, 2009년, pp.199 - 204  

Physiological and Biochemical Responses of Prorocentrum minimum to High Light Stress

Park, So-Yun    (South Sea Environment Research Department, KORDI   ); Choi, Eun-Seok    (Department of Genetic Engineering, Sungkyunkwan University   ); Hwang, Jin-Ik    (South Sea Environment Research Department, KORDI   ); Kim, Dong-Giun    (Department of Science Education, Kyungnam University   ); Ryu, Tae-Kwon    (Environmental Health Risk Research Department, NFRDI   ); Lee, Taek-Kyun    (South Sea Environment Research Department, KORDI  );
  • 초록

    Prorocentrum minimum is a common bloomforming photosynthetic dinoflagellate found along the southern coast of Korea. To investigate the adaptive responses of P. minimum to high light stress, we measured growth rate, and generation of reactive oxidative species (ROS), superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) in cultures exposed to normal (NL) and high light levels (HL). The results showed that HL (800 ${\mu}mol\;m^{-2}s^{-1}$ ) inhibited growth of P. minimum, with maximal inhibition after 7-9 days. HL also increased the amount of ROS and MDA, suggesting that HL stress leads to oxidative damage and lipid peroxidation in this species. Under HL, we first detected superoxide on day 4 and $H_2O_2$ on day 5. We also detected SOD activity on day 5 and CAT activity on day 6. The level of lipid peroxidation, an indicator of cell death, was high on day 8. Addition of diphenyleneiodonium (DPI), an NAD(P)H inhibitor, decreased the levels of superoxide generation and lipid peroxidation. Our results indicate that the production of ROS which results from HL stress in P. minimum also induces antioxidative enzymes that counteract oxidative damage and allow P. minimum to survive.

  • 주제어

    Prorocentrum minimum .   ROS .   superoxide dismutase .   catalase .   lipid peroxidation.  

  • 참고문헌 (32)

    1. Asada K (1984) Chloroplasts: Formation of active oxygen and its scavenging. Method Enzymol 10:422-429 
    2. Auh CK, Murphy TM (1995) Plasma Membrane Redox Enzyme Is Involved in the Synthesis of O$_{2}$ ? and H$_{2}$O$_{2}$ by Phytophthora Elicitor-Stimulated Rose Cells. Plant Physiol 107:1241-1247 
    3. Babior BM (1978) Oxygen-dependent microbial killing by phagocytes (second of two parts). N Engl J Med 298:721-725 
    4. Claiborne A (1985) Catalase activity. In: Greenwald RA (ed) CRC Handbook of Methods for Oxygen Radical Research, CRC Press, Boca Raton, Florida, pp 238-284 
    5. Cooper WJ, Zika RG (1983) Photochemical Formation of Hydrogen Peroxide in Surface and Ground Waters Exposed to Sunlight. Science 220:711-712 
    6. Fan C, Glibert PM (2005) Effects of light on nitrogen and carbon uptake during a Prorocentrum minimum bloom. Harmful Algae 4:629-641 
    7. Halliwell B, Gutteridge JM (1984) Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J 19:1-14 
    8. Kim CS, Lee SG., Lee CK, Kim HG, Jung J (1999) Reactive oxygen species as causative agents in the ichthyotoxicity of the red tide dinoflagellate Cochlodinium polykrikoides. J Plankton Res 21:2105-2115 
    9. McCord JM, Fridovich I (1969) Superoxide dismutase: An enzymic function for erythrocuprein (hemocuprein). J Biol Chem 244:6049-6055 
    10. Moon SG, Choi CM (2003) A list of important species and distribution of marine phytoplankton in Korea. J Environ Sci 12:725-733 
    11. Tanaka K, Yoshimatsu S, Shimada M (1992) Generation of superoxide anions by Chattonella antiqua: Possible causes for fish death caused by ‘Red Tide’. Cell Mol Life Sci 48:888-890 
    12. Twiner MJ (2000) Possible physiological mechanisms for production of hydrogen peroxide by the ichthyotoxic flagellate Heterosigma akashiwo. J Plankton Res 22:1961-1976 
    13. Zhang Y, Fu F-X, Whereat E, Coyne KJ, Hutchins DA (2006) Bottom-up controls on a mixed-species HAB assemblage: A comparison of sympatric Chattonella subsalsa and Heterosigma akashiwo (Raphidophyceae) isolates from the Delaware Inland Bays, USA. Harmful Algae 5:310-320 
    14. Ishimatsu A, Sameshima M, Tamura A, Oda T (1996) Histological analysis of the mechanisms of Chattonella-induced hypoximia in yellowtail. Fisheries Sci 62:50-58 
    15. Shalata A, Mittova V, Volokita M, Guy M, Tal M (2001) Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii to salt-dependent oxidative stress: The root antioxidative system. Physiol Plant 112:487-494 
    16. Groppa MD, Tomaro ML, Benavides MP (2001) Polyamines as protectors against cadmium or copper-induced oxidative damage in sunflower leaf discs. Plant Sci 161:481-488 
    17. Levine A, Tenhaken R, Dixon R, Lamb C (1994) H$_{2}$O$_{2}$ from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell 79:583-593 
    18. Grima EM, Sevilla JMF, Perez JAS, Camacho FG (1996) A study on simultaneous photolimitation and photoinhibition in dense microalgal cultures taking into account incident and averaged irradiances. J Biotech 45:59-69 
    19. Johnson K.J, Fantone JC, Kaplan J, Ward PA (1981) In vivo damage of rat lungs by oxygen metabolites. J Clin Invest 67:983-993 
    20. Johnston RB Jr, Godzik CA, Cohn ZA (1978) Increased superoxide anion production by immunologically activated and chemically elicited macrophages. J Exp Med 148:115-127 
    21. Dean RT (1987) Free radicals, membrane damage and cellmediated cytolysis. Br J Cancer Suppl 8:39-45 
    22. Greenberg JT, Guo A, Klessig DF, Ausubel FM (1994) Programmed cell death in plants: A pathogen-triggered response activated coordinately with multiple defense functions. Cell 77:551-563 
    23. Meloni DA, Oliva MA, Martinez CA, Cambraia J (2003) Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ Exp Bot 49:69-76 
    24. Kumar G., Knowles NR (1993) Changes in lipid peroxidation and lipolytic and free-radical scavenging enzyme activities during aging and sprouting of potato (Solanum tuberosum) seedtubers. Plant Physiol 102:115-124 
    25. Oda T, Ishimatsu A, Shimada M, Takeshita S, Muramatsu T (1992) Oxygen-radical-mediated toxic effects of the red tide flagellate Chattonella marina on Vibrio alginolyticus. Mar Biol 112:505-509 
    26. Sandstrom PA, Pardi D, Tebbey PW, Dudek RW, Terrian DM, Folks TM, Buttke TM (1995) Lipid hydroperoxide-induced apoptosis: lack of inhibition by Bcl-2 over-expression. FEBS Lett 365:66-70 
    27. Kawano I, Oda T, Ishimatsu A, Muramatsu T (1996) Inhibitory effect of the iron chelator desferrioxamine (Desferal) on the generation of activated oxygen species by Chattonella marina. Mar Biol 126:765-771 
    28. Oh SJ, Yoon YH, Kim DI, Shimasaki Y, Oshima Y, Honjo T (2006) Effects of light quantity and quality on the growth of the harmful Dinoflagellate, Cochlodinium polykrikoides Margalef (Dinophyceae). Algae 21:311-316     
    29. Serodio J, Vieira S, Cruz S (2008) Photosynthetic activity, photoprotection and photoinhibition in intertidal microphytobenthos as studied in situ using variable chlorophyll fluorescence. Cont Shelf Res 28:1363-137 
    30. Kakinuma K, Minakami S (1978) Effects of fatty acids on superoxide radical generation in leukocytes. Biochim Biophys Acta 538:50-59 
    31. Oda T, Akaike T, Hamamoto T, Suzuki F, Hirano T, Maeda H (1989) Oxygen radicals in influenza-induced pathogenesis and treatment with pyran polymer-conjugated SOD. Science 244:974-976 
    32. Palenik B, Zafiriou OC, Morel FMM (1987) Hydrogen peroxide production by a marine phytoplankton. Limnol Oceanogr 32:1365-1369 

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역


  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기