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환경독성학회지 = Journal of environmental toxicology v.24 no.4, 2009년, pp.303 - 309   피인용횟수: 1

Tetrabromobisphenol-A가 처리된 랫드의 간에서 항산화활성 평가
Evaluation of Hepatic Antioxidant Defense Systems in Rats Treated with Tetrabromobisphenol-A

이상윤    (충남대학교 약학대학 형질전환 복제돼지센터   ); 윤강욱    (충남대학교 약학대학 형질전환 복제돼지센터   ); 박선홍    (충남대학교 약학대학 형질전환 복제돼지센터   ); 정선기    (충남대학교 약학대학 형질전환 복제돼지센터   ); 강건욱    (조선대학교 약학대학   ); 정태천    (영남대학교 약학대학   ); 김형식    (부산대학교 약학대학   ); 정혜광    (충남대학교 약학대학 형질전환 복제돼지센터   ); 김봉희    (충남대학교 약학대학 형질전환 복제돼지센터   ); 김상겸    (충남대학교 약학대학 형질전환 복제돼지센터  );
  • 초록

    Hepatic antioxidant defense systems were examined in rats treated with tetrabromobisphenol-A (TBBPA), a brominated flame retardant, at the doses of 0, 250, 500 and 1,000 mg/kg for four weeks. Hepatic ratio of glutathione disulfide to glutathione (GSH) and levels of malondialdehyde, oxidative stress markers were not changed in rats treated with TBBPA. Hepatic expression of antioxidant enzymes including GSH peroxdiase-1 (GPX-1)/GSH reductase (GR), alpha-, mu- and pi-class glutathione-S-transferase (GST) and gamma-glutamylcysteine ligase catalytic subunit was determined using immunoblot analysis. Alpha-class GSTs, GPX-1 and GR levels were significantly decreased in rats treated with TBBPA at the dose of 500 or 1,000 mg/kg. These results show that TBBPA results in down-regulation of hepatic expression of antioxidant enzymes related with GSH, suggesting the liver in TBBPA-treated rats may be more sensitive to oxidants.


  • 주제어

    tetrabromobisphenol-A .   antioxidant defense system .   oxidative stress .   liver.  

  • 참고문헌 (20)

    1. Brigelius-Flohe R. Tissue-specific functions of individual glutathione peroxidase, Free Radical Biol Med 1999; 27: 951-965 
    2. Canesi L, Lorusso LC, Ciacci C, Betti M and Gallo G. Effects of the brominated flame retardant tetrabromobis-phenol-A (TBBPA) on cell signaling and function of Mytilus hemocytes: Involvement of MAP kinases and protein kinase C, Aquat Toxicol 2005; 75: 277-287 
    3. Kim SK, Woodcroft KJ, Khodadadeh SS and Novak RF. Insulin signaling regulates gamma-glutamylcysteine ligase catalytic subunit expression in primary cultured rat hepatocytes, J Pharmacol Exp Ther 2004; 311: 99-108 
    4. Mannervik B, Alin P, Guthenberg C, Jensson HM, Tahir K, Warhohm M and Jornvall H. Identification of three classes of cytosolic glutathione transferase common to several mammalian species: correlation between structural data and enzymatic properties, Proc Natl Acad Sci USA 1985; 82: 7202-7206 
    5. Sun W, Kalen AL, Smith BJ, Cullen JJ and Oberley LW. Enhancing the antitumor activity of adriamycin and ionizing radiation, Cancer Res 2009; 69: 4294-4300 
    6. Serviddio G, Romano1 AD, Gesualdo L, Tamborra1 R, Palma AM, Rollo T, Altomare E and Vendemiale G. Ursodeoxycholic acid protects against secondary biliary cirrhosis by preventing mitochondrial oxidative stress, Hepatology 2004; 39: 711-720 
    7. Casalino E, Calzaretti G, Landriscina M, Sblano C, Fabiano A and Landriscina C. The Nrf2 transcription factor contributes to the induction of alpha-class GST isoenzymes in liver of acute cadmium or manganese intoxicated rats: comparison with the toxic effect on NAD(P)H: quinone reductas, Toxicology 2007; 237: 24-34 
    8. Kim SK and Novak RF. The role of intracellular signaling in insulin-mediated regulation of drug metabolizing enzyme gene and protein expression, Pharmacol Ther 2007; 113: 88-120 
    9. Sun Q, Komura S, Ohishi N and Yagi K. Alpha-class isozymes of glutathione S-transferase in rat liver cytosol possess glutathione peroxidase activity toward phospholipid hydroperoxide, Biochem Mol Biol Int 1996; 39: 343-352 
    10. Ursini F, Maiorino M, Brigelius-Flohe R, Aumann KD, Roveri A, Schomburg D and Flohe L. Diversity of glutathione peroxidase, Methods Enzymol 1995; 252: 38-53 
    11. Townsend DM and Tew KD. The role of glutathione-S-transferase in anti-cancer drug resistance, Oncogene 2003; 22: 7369-7375 
    12. Lu SC. Regulation of hepatic glutathione synthesis: current concepts and controversies, FASEB J 1999; 13: 1169-1183 
    13. Darnerud PO. Toxic effects of brominated flame retardants in man and in wildlife, Environ Int 2003; 29: 841-853 
    14. Kemmleina S, Herzkeb D and Law RJ. BFR-governmental testing programme, Environ Int 2003; 29: 781-792 
    15. Kim SK, Yang JW, Kim MR, Roh SH, Kim HG, Lee KY, Jeong HG and Kang KW. Increased expression of Nrf2/ ARE-dependent anti-oxidant proteins in tamoxifen-resistant breast cancer cells, Free Radic Biol Med 2008; 45: 537-546 
    16. Ketterer B. Glutathione S-transferases and prevention of cellular free radical damage, Free Radic Res 1998; 28: 647-658 
    17. Takebe G, Yarimizu J, Saito Y, Hayashi T, Nakamura H, Yodoi J, Nagasawa S and Takahashi K. Comparative study on the hydroperoxide and thiol specificity of the glutathione peroxidase family and selenoprotein P, J Biol Chem 2002; 277: 41254-41258 
    18. Thomsen C, Lundanes E and Becher G. Brominated flame retardants in archived serum samples from Norway: A study on temporal trends and the role of age, Environ Sci Technol 2002a; 36: 1414-1418 
    19. Kim SK, Woodcroft KJ, Oh SJ, Abdelmegeed MA and Novak RF. Role of mechanical and redox stress in activation of mitogen-activated protein kinases in primary cultured rat hepatocytes, Biochem Pharmacol 2005; 70: 1785-1795 
    20. Thomson C, Leknes H, Lundanes E and Becher G. A new method for determination of halogenated flame retardants in human milk using solid-phase extraction, J Anal Toxicol 2002b; 26: 129-137 
  • 이 논문을 인용한 문헌 (1)

    1. Kim, In-Hye ; Kim, Jong-Bae ; Cho, Kang-Jin ; Kim, Jae-Hyun ; Om, Ae-Son 2012. "Cytoprotective Effect of Ethanol Extract from Maesil (Prunus mume Sieb. et Zucc.) on Alloxan-induced Oxidative Damage in Pancreatic-cell, HIT-T15" 韓國資源植物學會誌 = Korean journal of plant resources, 25(2): 184~192     

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