본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

질소 화합물이 담배연기성분 및 안전성에 미치는 영향
Effect of nitrogen compounds on the chemical composition and biological activity of mainstream smoke

신한재    (KT&G 중앙연구원   ); 박철훈    (KT&G 중앙연구원   ); 손형옥    (KT&G 중앙연구원   ); 이형석    (KT&G 중앙연구원   ); 김용하    (KT&G 중앙연구원   ); 현학철    (KT&G 중앙연구원  );
  • 초록

    The objective of this study was to investigate the effect of nitrogen compounds such as protein on the chemical composition and toxicity of cigarette mainstream smoke. BSA protein was treated into the tobacco leaf of original 2R4F cigarette at 1~4 % level. The studies were performed which included a bacterial mutagenicity assay and a mammalian cell cytotoxicity assay for total particulate matter(TPM), and glutathione(GSH) consumption assay for gas/vapor phase(GVP) and determination of smoke chemical constitute. Cigarettes treated with protein were observed dose-dependent increase in yield of volatiles, semi-volatiles and aromatic amines compared with control cigarette. However, carbonyl compounds such as acrolein was lower than that of control cigarette when calculated on an equal TPM basis. The cytotoxicity of TPM obtained from the protein-added cigarettes was not different from that of control cigarette. However, the mutagenicity of the TPM from protein-treated cigarettes(1~4 %) was up to 10-27 % higher than that of control. On the other hand, toxicity of GVP from protein-treated cigarette(4 %) was significantly decreased compared with control cigarette. An overall assessment of our data suggests that nitrogen compounds such as protein should be important for the chemical composition and biological activity of cigarette mainstream smoke.


  • 주제어

    Total particulate matter .   gas-vapor phase .   mutagenicity .   cytotoxicity.  

  • 참고문헌 (30)

    1. Bombick, D. W., Ayres, P. H., Putnam, K., Bombick, B. R. and Doolittle, D. J. (1998) Chemical and biological studies of a new cigarette that primarily heats tobacco. Part 3. In vitro toxicity of whole smoke. Food Chem. Toxicol. 36: 191-197 
    2. Health Canada (1999c). Determination of pyridine, quinoline and styrene in mainstream tobacco smoke. Official method T-112 
    3. Hamilton, J. L. and Lowe, R. H. (1978) Changes in the concentrations of proteins, amino acids and ammonia in burley tobacco during air curing. Tob. Sci., 22, 89-93 
    4. Phillips, D. H. (2002) Smoking-related DNA and protein adducts in human tissues. Carcinogenesis 23: 1979-2004 
    5. Tewes, F. J., Meisgen, T. J., Veltel, D. J., Roemer, E. and Patskan, G. (2003) Toxicological evaluation of electrically heated cigarette. Part 3: genotoxicity and cytotoxicity of mainstream smoke. J. Appl. Toxicol. 23: 341-348 
    6. Andreoli, C., Gigante, D. and Nunziata, A. (2003) A review of in vitro methods to assess the biological activity of tobacco smoke with the aim of reducing the toxicity of smoke. Toxicol. in Vitro 17: 587-594 
    7. ISO Standard 4387, third ed., (2000a) International Organization for Standardization. Cigarettesdetermination of total and nicotine-free dry particulate matter using a routine analytical smoking machine 
    8. Leffingwell, J. C. and Leffingwell, D. (1988) Chemical and sensory aspects of tobacco flavor. Rec. Adv. Tob. Sci., 14, 169-218 
    9. Manabe, S. and Wada, O. (1990) Carcinogenic tryptophan pyrolysis products in cigarette smoke condensate and cigarette smokepolluted indoor air. Environ. Pollute. 64: 121-132 
    10. Park, C. H., Sohn, H. O., Shin, H. J., Lee, H. S., Min, Y. K. and Hyun, H. C. (2007) Toxicity assessment of gas phase in cigarette smoke using cell-free assay. J. Kor. Soc. Tob. Sci. 29: 110-117     
    11. Putnam, K. P., Bombick, D. W. and Doolittle D. J. (2002) Evaluation of eight in vitro assays for assessing the cytotoxicity of cigarette smoke condensate. Toxicol. in Vitro 16: 599-607 
    12. Curvall, M., Enzell, C. R. and Pettersson, B. (1984) An evaluation of the utility of four in vitro short term tests for predicting the cytotoxicity of individual compounds derived from tobacco smoke. Cell Biol. Toxicol. 1: 173-193 
    13. Doolittle, D. J., Lee, C. K., Ivett, J. L., Mirsalis, J. C., Riccio, E., Rudd, C. J., Burger, G. T. and Hayes A. W. (1990) Comparative studies on the genotixic activity of mainstream smoke condensate from cigarettes which burn or only heat tobacco. Environ. Mo. Mutag. 15: 93-105 
    14. Health Canada (1999b). Determination of 1,3-butadiene, isoprene, acrylonitrile, bensene, and toluene in mainstream tobacco smoke. Official method T-116 
    15. ISO Standard ISO 10315, second ed. and Corrgendum I, (2000c) International Organization for Standardization. Cigarettes determination of nicotine in smoke condensates-gas chromatographic method 
    16. Health Canada (2004). Neutral Red Uptake Assay for Mainstream Tobacco Smoke. Official method T-502 
    17. Maron, D. M. and Ames, B. N. (1983) Revised methods for the Salmonella mutagenicity test. Mutat. Res. 113: 173-215 
    18. Bombick, D. W., Bombick, B. R. Ayres, P. H., Putnam, K., Avalos, J., Borgerding, M. F. and Doolittle, D. J. (1997) Evaluation of the genotoxic and cytotoxic potential of mainstream whole smoke and smoke condensate from a cigarette containing a novel carbon filter. Fund. Appl. Toxicol. 39: 11-17 
    19. DeMarini, D. M. (1983) Genotoxicity of tobacco smoke and tobacco smoke condensate. Mutat. Res. 114: 59-89 
    20. Shin, H. J., Sohn, H. O., Park, C. H., Lee, H. S., Min, Y. K. and Hyun, H. C. (2007) Evaluation of the in vitro biological activity of selected 35 chemicals. J. Kor. Soc. Tob. Sci. 29: 30-40     
    21. ISO Standard 3308, fourth ed., (2000b) International Organization for Standardization. Routine analytical cigarette smoking machine-definitions and standard conditions 
    22. Rodgman, A. and Green, C. R. (2003) Toxic chemicals in cigarette mainstream smoke-hazard and hoopla. Beitrage zur Tabakforschung 20: 481-545 
    23. Cahours, X., Blanchet, M, Haond, C., Marchand V. and Dumery, B. (2006) A cell-free method to assess the gaseous phase cytotoxicity of cigarette smoke. Coresta Conference Paris, France 4 
    24. ISO Standard 3402, fourth ed., (1999) International Organization for Standardization. Tobacco and tobacco products-atmosphere for conditioning and testing 
    25. Hamilton, J. L.(1974) Changes during curing of burley tobacco. PhD dissertation, University of Kentucky, Xerox University Microfilms, Ann Arbor, Michigan 
    26. Wynder, E. L. and Hoffmann, D. (1967) Tobacco and tobacco smoke; studies in experimental carcinogenesis, Academic Press, New York 
    27. Cooper, R. L., Lindsey, A. J. and Wallwe, R. E. (1954) The presence of 3,4-benzopyrene in cigarette smoke. Chem. Ind. 46: 14-18 
    28. Shmuk, A. A. (1953) The Chemistry and Technology of Tobacco. Pishchepromedadat, Moscow 
    29. Borenfreund, E. and Puerner, J. A. (1985) Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol. Lett. 24: 119-124 
    30. Health Canada (1999a) Determination of selected carbonyls in mainstream tobacco smoke. Tobacco Control Programen Health Canada-Official method T-104 
  • 이 논문을 인용한 문헌 (1)

    1. Park, Jong-Ho ; Hong, Sung-Jun ; Han, Eun-Jung ; Shim, Chang-Ki ; Kim, Min-Jeong ; Lee, Min-Ho ; Kim, Yong-Ki 2014. "Effect of Tobacco Smoke on Controlling Aphids in Greenhouse" 한국응용곤충학회지 = Korean journal of applied entomology, 53(4): 409~413     

 저자의 다른 논문

  • 신한재 (15)

    1. 2003 "홍삼추출잔사로부터 항암면역조절 활성을 보여주는 홍삼산성다당체(RGAP)의 분리 및 이화학적 특성" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition 32 (5): 752~757    
    2. 2003 "홍삼산성다당체 (RGAP)와 항암제의 병용투여에 의한 항암시너지 효과" Journal of ginseng research = 高麗人參學會誌 27 (2): 47~51    
    3. 2003 "세포주에 따른 담배연기응축물의 소핵생성 비교" 한국연초학회지 = Journal of the Korean society of tobacco science 25 (2): 128~136    
    4. 2004 "담배연기응축물의 소핵생성 측정시 두가지 방법간의 민감성 비교" 한국연초학회지 = Journal of the Korean society of tobacco science 26 (2): 152~158    
    5. 2005 "향유와 꽃향유 향기성분의 생리활성 검정" 한국연초학회지 = Journal of the Korean society of tobacco science 27 (1): 19~30    
    6. 2005 "담배주류연의 세포독성에 대한 담배필터의 영향" 한국연초학회지 = Journal of the Korean society of tobacco science 27 (1): 51~58    
    7. 2005 "홍삼산성다당체 (RGAP)의 경구투여에 의한 항종양 효과" Journal of ginseng research = 高麗人參學會誌 29 (4): 176~181    
    8. 2007 "Toxicity Assessment of Gas Phase in Cigarette Smoke Using Cell-free Assay" 한국연초학회지 = Journal of the Korean society of tobacco science 29 (2): 110~117    
    9. 2007 "35종의 특정 화학성분들의 in vitro 활성 평가" 한국연초학회지 = Journal of the Korean society of tobacco science 29 (1): 30~40    
    10. 2008 "담배 주류연의 생물학적 활성에 대한 흡연조건의 영향" 한국연초학회지 = Journal of the Korean society of tobacco science 30 (1): 14~24    
  • Park, Chul-Hoon (10)

  • 손형옥 (11)

  • 이형석 (7)

  • 김용하 (13)

  • 현학철 (10)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드
  • 원문이 없습니다.

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 이용한 콘텐츠
이 논문과 함께 출판된 논문 + 더보기