본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Alkaline $\alpha$-amylase Production from Bacillus megaterium

Jia, Shiru    (Biochemical Engineering Lab., Tianjin University of Science & Technology   ); Lim, Chae-kyu    (Department of Herbal Medicine Resources Development, Naju College   ); Seo, Gwang-Yeob    (Health and Environment Research Institute of Gwangju city   ); Nam, Hyung-Gun    (Department of Biochemical Engineering, Chosun University  );
  • 초록

    The enzyme expressed from strain L-49 was 2.01 times higher than that of original strain. Strain L-49 can grow on culture plate with $50{\mu}g/mL$ ampicillin. The synthesis of $\alpha$ -amylase was greatly suppressed when strain L-49 was grown on monosaccharide such as glucose and polysaccharide at the same time cell concentration was low. Amylase production was enhanced when the bacterium was grown on starch and dextrin. Among different nitrogen sources tried, yeast extract was found to be the best followed by panpeptone, peptone, meat extract, bean meal, and corn steep liquor. The average rate of enzyme production was enhanced for 3~4 times in fermentation time from 24h to 44h. The sugar uptake rate has also increased. Low oxygen supply rate enhanced the rate of strain propagation but depressed the enzyme production. Hence it is benefit to obtain high enzyme activity that agitation speed maintained not lower than 400r/min and aeration rate maintained greater than 1:1vvm.


  • 참고문헌 (5)

    1. Pratima, B. and Pramod, K. P.: High-temperature alkaline alpha-amylase from Bacillus lichemfomlls TCRDC-B13, Biotech. Bioeng., 33, pp.72-78, (1989) 
    2. Kaztunasa, H., Yamane, K and Maruo, B.: Stepwise introduction of regulatory genes stimulating production of alpha-amylase into Bacillus. Agr. BioI. Chem., 43, pp.2343-2349, (1979) 
    3. Jia, S.,Choe, Y. D., Cho, H.: Isolation and identification of Bacillus megaterium producing Nkaline $\alpha$-amylase, J. Enviro. San. Eng.,23(1), pp.25-31, (2008) 
    4. Medda, S. and Chandra, A. K.: New strains of BaCillus Iicheniformis and Bacillus coagulans producing thenmstable alpha-amylase active at alkaline pH.J. Appl. Bacteriol., 48, pp.47-58, (1980) 
    5. Buchanan, K. E. and Gibbons, E. S.: Bergey's Manual of Determinative Bacteriology, 8th Ed., Baltimore, (1974) 

 저자의 다른 논문

  • Lim, Chae-Kyu (6)

    1. 1998 "한국산 충생자낭균류의 미기록종에 관하여" 한국균학회지 = The Korean journal of mycology 26 (3): 302~305    
    2. 2002 "Interesting Species of the Laboulbeniales from Upo Swamp" Mycobiology 30 (3): 128~132    
    3. 2003 "한국의 집게벌레목에 기생하는 충생자낭균류의 분류에 관하여" 한국균학회지 = The Korean journal of mycology 31 (3): 129~133    
    4. 2003 "Estimation of Cell Concentration by Light Transmitter During the Culture of Methylotrophic Yeast Pichia pastoris" Mycobiology 31 (4): 226~228    
    5. 2008 "Mutagenesis of Streptomyces kasugaensis for Kasugamycin Production" 환경위생공학 = Journal of environmental and Sanitary engineering 23 (4): 23~29    
  • 남형근 (6)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역

원문보기

무료다운로드
  • NDSL :
유료다운로드

유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기