본문 바로가기
HOME> 논문 > 논문 검색상세

논문 상세정보

Journal of microbiology and biotechnology v.20 no.6, 2010년, pp.968 - 973   SCIE
본 등재정보는 저널의 등재정보를 참고하여 보여주는 베타서비스로 정확한 논문의 등재여부는 등재기관에 확인하시기 바랍니다.

Characterization of Biochemical Properties of Feline Foamy Virus Integrase

Lee, Dong-Hyun    (Department of Biotechnology, Chung-Ang University   ); Hyun, U-Sok    (Department of Biotechnology, Chung-Ang University   ); Kim, Ji-Ye    (Department of Biotechnology, Chung-Ang University   ); Shin, Cha-Gyun    (Department of Biotechnology, Chung-Ang University  );
  • 초록

    In order to study its biochemical properties, the integrase (IN) protein of feline foamy virus (FFV) was overexpressed in Escherichia coli, purified by two-step chromatography, (Talon column and heparin column), and characterized in biochemical aspects. For the three enzymatic reactions of the 3'-processing, strand transfer, and disintegration activities, the $Mn^{2+}$ ion was essentially required as a cofactor. Interestingly, $Co^{2+}$ and $Zn^{2+}$ ions were found to act as effective cofactors, whereas other transition elements such as $Ni^{2+}$ , $Cu^{2+}$ , $La^{3+}$ , $Y^{3+}$ , $Cd^{2+}$ , $Li^{1+}$ , $Ba^{2+}$ , $Sr^{2+}$ , and $V^{3+}$ were not. Regarding the substrate specificity, FFV IN has low substrate specificities as it cleaved in a significant level prototype foamy virus (PFV) U5 LTR substrate as well as FFV U5 LTR substrate, whereas PFV IN did not. Finally, the 3'-processing activity was observed in high concentrations of several solvents such as CHAPS, glycerol, Tween 20, and Triton X-100, which are generally used for dissolution of chemicals in inhibitor screening. Therefore, in this first report showing its biochemical properties, FFV IN is proposed to have low specificities on the use of cofactor and substrate for enzymatic reaction as compared with other retroviral INs.

  • 주제어

    Integrase .   feline foamy virus .   transition element .   cofactor .   specificities.  

  • 참고문헌 (29)

    1. Achong, B. G., W. A. Mansell, M. A. Epstein, and P. Clifford. 1971. An unusual virus in cultures from a human nasopharyngeal carcinoma. J. Natl. Cancer Inst. 46: 299-307. 
    2. Appa, R. S., C.-G. Shin, P. Lee, and S. A. Chow. 2001. Role of the nonspecific DNA-binding region and $\alpha$ helices within the core domain of retroviral integrase in selecting target DNA sites for integration. J. Biol. Chem. 276: 45846-45855. 
    3. Bujacz, G., J. Alexandratos, and A. Wlodawer. 1997. Binding of different divalent cations to the active site of avian sarcoma virus integrase and their effects on enzymatic activity. J. Biol. Chem. 272: 18161-18168. 
    4. Bushman, F. D. and R. Craigie. 1990. Sequence requirements for integration of Moloney murine leukemia virus DNA in vitro. J. Virol. 64: 5645-5648. 
    5. Chaga, G., J. Hopp, and P. Nelson. 1999. Immobilized metal ion affinity chromatography on $Co^{2+}$-carboxymethyl aspartate-agarose Superflow, as demonstrated by one-step purification of lactate dehydrogenase from chicken breast muscle. Biotechnol. Appl. Biochem. 29: 19-24. 
    6. Chow, S. A., K. A. Vincent, V. Ellison, and P. O. Brown. 1992. Reversal of integration and DNA splicing mediated by integrase of human immunodeficiency virus. Science 255: 723-726. 
    7. Ciuffi, A. and F. D. Bushman. 2006. Retroviral DNA integration: HIV and the role of LEDGF/p75. Trends Genet. 22: 388-395. 
    8. Craigie, R., T. Fujuwara, and F. Bushman. 1990. The IN protein of Moloney murine leukemia virus processes the viral DNA ends and accomplishes their integration in vitro. Cell 62: 829-837. 
    9. Delelis, O., K. Carayon, E. Guiot, H. Leh, P. Tauc, J.-C. Brochon, J.-F. Mouscadet, and E. Deprez. 2008. Insight into the integrase-DNA recognition mechanism. J. Biol. Chem. 283: 27838-27849. 
    10. Drelich, M., R. Wilhelm, and J. Mous. 1992. Identification of amino acid residues critical for endonuclease and integration activities of HIV-1 protein in vitro. Virology 188: 459-468. 
    11. llison, V. and P. O. Brown. 1994. A stable complex between integrase and viral DNA ends mediates HIV integration in vitro. Proc. Natl. Acad. Sci. U.S.A. 91: 7316-7320. 
    12. Enders, J. and T. Peebles. 1954. Propagation in tissue culture of cytopathogenic agents from patients with measles. Proc. Soc. Biol. Med. 86: 277-287. 
    13. Ernest, A.-A. and A. M. Skalka. 1997. A metal-induced conformational change and activation of HIV-1 integrase. J. Biol. Chem. 272: 16196-16205. 
    14. Geiselhart, V., A. Schwantes, P. Bastone, M. Frech, and M. Lochelt. 2003. Features of the Env leader protein and the Nterminal Gag domain of feline foamy virus important for virus morphogenesis. J. Virol. 310: 235-244. 
    15. Kang, S. Y., D. G. Ahn, C. Lee, Y. S. Lee, and C.-G. Shin. 2008. Functional nucleotides of U5 LTR determining substrate specificity of prototype foamy virus integrase. J. Microbiol. Biotechnol. 18: 1044-1049.     
    16. Katzman, M. and M. Sudol. 1994. In vitro activities of purified visna virus intergase. J. Viol. 68: 3558-3569. 
    17. Kawasuji, T., M. Fuji, T. Yoshinaga, A. Sato, T. Fujiwara, and R. Kiyama. 2006. A platform for designing HIV integrase inhibitors. Part 2: A two-metal binding model as a potential mechanism of HIV integrase inhibitors. Bioorg. Med. Chem. 14: 8420-8429. 
    18. Kulkosky, J., K. S. Jones, R. A. Katz, J. P. Mack, and A. M. Skalka. 1992. Residues critical for retroviral integrative recombination in a region that is highly conserved among retroviral/retrotransposon integrases and bacterial insertion sequence transposases. Mol. Cell. Biol. 12: 2331-2338. 
    19. Lacellier, C.-H. and A. Saib. 2000. Minireview - Foamy viruses: Between retroviruses and pararetroviruses. J. Virol. 271: 1-8. 
    20. Lafemina, R. L., P. L. Callahan, and M. G. Cordingley. 1991. Substrate specificity of recombinant human immunodeficiency virus integrase protein. J. Virol. 65: 5624-5630. 
    21. Murray, S. M. and M. L. Linial. 2006. Foamy virus infection in primates. J. Med. Primatol. 35: 225-235. 
    22. Pahl, A. and R. M. Flugel. 1993. Endonucleolytic cleavages and DNA-joining activities of the integration protein of human foamy virus. J. Virol. 67: 5426-5434. 
    23. Pahl, A. and R. M. Flugel. 1995. Characterization of the human spumaretrovirus integrase by site-directed mutagenesis, by complementation analysis, and by swapping the zinc finger. J. Biol. Chem. 270: 2957-2966. 
    24. Reicin, A. S., G. Kalpana, S. Paik, S. Marmon, and S. P. Goff. 1995. Sequence in the human immunodeficiency virus type 1 U3 region required for in vivo and in vitro integration. J. Virol. 69: 5904-5907. 
    25. Roth, M. J., P. L. Schwartzberg, and S. P. Goff. 1989. Structure of the termini of DNA intermediates in the integration of retroviral DNA: Dependence on IN function and terminal DNA sequence. Cell 58: 47-54. 
    26. Shibata, K., Y. Morita, S. Abe, B. Stankovic, and E. Davies. 1999. A pyrase from pea stems: Isolation, purification, characterization and identification of an NTPase from the cytoskeleton fraction of pea stem tissue. Plant Physiol. Biochem. 37: 1-8. 
    27. Van Gent, D. C., C. Vink, A. A. Groeneger, and R. H. Plasterk. 1993. Complementation between HIV integrase proteins mutated in different domains. EMBO J. 12: 3261-3267. 
    28. Vincent, K. A., V. Ellison, S. A. Chow, and P. O. Brown. 1993. Characterization of human immunodeficiency virus type 1 integrase expressed in Escherichia coli and analysis of variants with amino-terminal mutation. J. Virol. 67: 425-437. 
    29. Murphy, J. E., T. De Los Santos, and S. P. Goff. 1993. Mutational analysis of the sequences at the termini of the Moloney murine leukemia virus DNA required for integration. Virology 195: 432-440. 

 저자의 다른 논문

  • 이동현 (1)

    1. 2008 "원조포미바이러스 U5 LTR 말단의 보존적인 잔기의 돌연변이에 대한 인테그라제의 반응성" 약학회지 = Yakhak hoeji 52 (2): 125~130    
  • 현우석 (1)

  • Kim, Ji-Ye (1)

  • Shin, Cha-Gyun (25)

 활용도 분석

  • 상세보기

    amChart 영역
  • 원문보기

    amChart 영역



유료 다운로드의 경우 해당 사이트의 정책에 따라 신규 회원가입, 로그인, 유료 구매 등이 필요할 수 있습니다. 해당 사이트에서 발생하는 귀하의 모든 정보활동은 NDSL의 서비스 정책과 무관합니다.

원문복사신청을 하시면, 일부 해외 인쇄학술지의 경우 외국학술지지원센터(FRIC)에서
무료 원문복사 서비스를 제공합니다.

NDSL에서는 해당 원문을 복사서비스하고 있습니다. 위의 원문복사신청 또는 장바구니 담기를 통하여 원문복사서비스 이용이 가능합니다.

이 논문과 함께 출판된 논문 + 더보기