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Journal of microbiology and biotechnology v.20 no.6, 2010년, pp.1001 - 1005   SCIE
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Purification and Characterization of Chitinase from a New Species Strain, Pseudomonas sp. TKU008

Wang, San-Lang    (Life Science Development Center, Tamkang University   ); Lin, Bo-Shyun    (Graduate Institute of Life Sciences, Tamkang University   ); Liang, Tzu-Wen    (Life Science Development Center, Tamkang University   ); Wang, Chuan-Lu    (Department of Cosmetic Science and Application, Lan-Yang Institute of Technology   ); Wu, Pei-Chen    (Life Science Development Center, Tamkang University   ); Liu, Je-Ruei    (Graduate Institute of Biotechnology, National Taiwan University  );
  • 초록

    The chitinase-producing strain TKU008 was isolated from soil in Taiwan, and it was identified as a new species of Pseudomonas. The culture condition suitable for production of chitinase was found to be shaking at $30^{\circ}C$ for 4 days in 100 ml of medium containing 1% shrimp and crab shell powder, 0.1% $K_2HPO_4$ , and 0.05% $MgSO_4{\cdot}7H_2O$ (pH 7). The TKU008 chitinase was suppressed by the simultaneously existing protease, which also showed the maximum activity at the fourth day of incubation. The molecular mass of the chitinase was estimated to be 40 kDa by SDS-PAGE. The optimum pH, optimum temperature, pH stability, and thermal stability of the chitinase were pH 7, $50^{\circ}C$ , pH 6-7, and TEX>$50^{\circ}C$ , respectively. The chitinase was completely inhibited by $Mn^{2+}$ and $Cu^{2+}$ . The results of peptide mass mapping showed that 11 tryptic peptides of the chitinase were identical to the chitinase CW from Bacillus cereus (GenBank Accession No. gi 45827175) with a 32% sequence coverage.


  • 주제어

    Pseudomonas .   chitinase .   chitin .   shrimp and crab shell wastes.  

  • 참고문헌 (9)

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    5. Wang, S. L., C. H. Chao, T. W. Liang, and C. C. Chen. 2009. Purification and characterization of protease and chitinase from Bacillus cereus TKU006 and conversion of marine wastes by these enzymes. Mar. Biotechnol. 11: 334-344. 
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    7. Wang, S. L., S. J. Chen, and C. L. Wang. 2008. Purification and characterization of chitinases and chitosanases from a new species strain Pseudomonas sp. TKU015 using shrimp shells as a substrate. Carbohydr. Res. 343: 1171-1179. 
    8. Wang, S. L. and W. T. Chang. 1997. Purification and characterization of two bifunctional chitinase/lysozymes extracellularly produced by Pseudomonas aeruginosa K-187 in a shrimp and crab shell powder medium. Appl. Environ. Microbiol. 63: 380-386. 
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 저자의 다른 논문

  • Wang, San-Lang (1)

    1. 2010 "Conversion of Shrimp Shell by Using Serratia sp. TKU017 Fermentation for the Production of Enzymes and Antioxidants" Journal of microbiology and biotechnology 20 (1): 117~126    

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